Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,903	C→T	D405N (GAC→AAC)	caiB ←	crotonobetainyl CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,903	0	C	T	100.0%	180.5 / NA	58	D405N (GAC→AAC)	caiB	crotonobetainyl CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/26);  total (32/26)

AAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGT  >  NC_000913/37777‑38028                                                                                                                               |                                                                                                                              aaGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGc                                                                                                                   <  1:1282203/139‑1 (MQ=255)    aTCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAg                                                                                                                >  1:280256/1‑139 (MQ=255)      cGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                              <  2:280256/139‑1 (MQ=255)                  gACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCa                                                                                                  >  1:105443/1‑139 (MQ=255)                   aCGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                        >  2:341873/1‑116 (MQ=255)                   aCGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                        <  1:341873/116‑1 (MQ=255)                           gttgtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGaa                                                                                         <  2:105443/139‑1 (MQ=255)                             tgtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                              <  2:980659/116‑1 (MQ=255)                             tgtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                              >  1:980659/1‑116 (MQ=255)                              gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGt                                                                                      >  1:726762/1‑139 (MQ=255)                                   ccAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGttt                                                                                 >  1:819813/1‑139 (MQ=255)                                    cAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGtttt                                                                                <  2:726762/139‑1 (MQ=255)                                       tGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGc                                                                             >  1:540077/1‑139 (MQ=255)                                             ccATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                        >  2:983346/1‑90 (MQ=255)                                             ccATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                        <  1:983346/90‑1 (MQ=255)                                                 tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCCACTCCTGAATGTCGTTTTCGCTGTAGCCGat                                                                   >  2:1513462/1‑139 (MQ=255)                                                  gcacttttgtcgaTTTTTGTGCGTTTGTTGTTGGGCGGGGGCTAGTCAGGCAAGCCGACGGACGGCACGCGTTTAGTTCTCAACGTTGGCCAGACCTTTGCTGACCAGCTCGTGAATGTCGTTTTCGCTGTAGCCGata                                                                  <  1:290873/127‑1 (MQ=255)                                                   cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGatat                                                                 >  2:897611/1‑139 (MQ=255)                                                        ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAc                                                                                                    <  2:474656/99‑1 (MQ=255)                                                        ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAc                                                                                                    >  1:474656/1‑99 (MQ=255)                                                        ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTc                                                            >  2:1319817/1‑139 (MQ=255)                                                           tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCaaa                                                         >  1:975325/1‑139 (MQ=255)                                                                ttttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCaa                                                                                                 >  1:1168055/1‑94 (MQ=255)                                                                ttttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCaa                                                                                                 <  2:1168055/94‑1 (MQ=255)                                                                ttttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGc                                                    >  1:1096099/1‑139 (MQ=255)                                                                ttttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGc                                                    >  2:230463/1‑139 (MQ=255)                                                                  ttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAg                                                  >  1:1520115/1‑139 (MQ=255)                                                                   tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                 >  2:154968/1‑139 (MQ=255)                                                                   tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                 <  1:154968/139‑1 (MQ=255)                                                                         tttGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                           <  1:230463/139‑1 (MQ=255)                                                                         tttGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                           <  1:16652/139‑1 (MQ=255)                                                                               aTTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatgcc                                     <  2:153982/139‑1 (MQ=255)                                                                                  gggcgggcGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatgccatg                                  <  1:897611/139‑1 (MQ=255)                                                                                     cgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGTCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCaaatgg                                                      >  1:1790533/1‑113 (MQ=255)                                                                                     cgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGTCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCaaatgg                                                      <  2:1790533/116‑4 (MQ=255)                                                                                     cgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                 <  2:1637270/121‑1 (MQ=255)                                                                                     cgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGa                               >  2:798384/1‑139 (MQ=255)                                                                                     cgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTCGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                 >  1:1637270/1‑121 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                   >  1:1499598/1‑69 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                   <  2:1499598/69‑1 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCAtgt                                 <  1:1125982/135‑1 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCAtgt                                 >  2:1125982/1‑135 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAgg                             >  1:308601/1‑139 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAgg                             >  2:1496907/1‑139 (MQ=255)                                                                                       ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCC‑TGTGAggg                            >  2:607796/1‑139 (MQ=255)                                                                                        gcgcTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                   >  1:772375/1‑68 (MQ=255)                                                                                        gcgcTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                   <  2:772375/68‑1 (MQ=255)                                                                                             aGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTc                       <  1:798384/139‑1 (MQ=255)                                                                                                  ggCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                 <  1:804831/108‑1 (MQ=255)                                                                                                  ggCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                 >  2:804831/1‑108 (MQ=255)                                                                                                   gCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGcc                 >  1:1704685/1‑139 (MQ=255)                                                                                                      aGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCTATGCCATGTGAGGGCATTCCGCGCCAGa              >  1:1137404/1‑139 (MQ=255)                                                                                                      aGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGa              <  2:1704685/139‑1 (MQ=255)                                                                                                        ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGAtt            >  1:1614238/1‑139 (MQ=255)                                                                                                                 gCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCgggg   <  2:516451/139‑1 (MQ=255)                                                                                                                  ccACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGTGCCAGATTTGTCCGGGGt  <  1:1496907/139‑1 (MQ=255)                                                                                                                     cgcgTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATgg                                                     >  2:1040925/1‑85 (MQ=255)                                                                                                                     cgcgTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATgg                                                     <  1:1040925/85‑1 (MQ=255)                                                                                                                               |                                                                                                                              AAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGT  >  NC_000913/37777‑38028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑