Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,317,409	C→T	G163S (GGC→AGC)	phnL ←	ribophosphonate triphosphate synthase subunit, putative ABC transporter‑related ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,317,409	0	C	T	98.0%	156.1 / NA	50	G163S (GGC→AGC)	phnL	ribophosphonate triphosphate synthase subunit, putative ABC transporter‑related ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/22);  minor base A (1/0);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAGATGCCTACGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGT  >  NC_000913/4317276‑4317544                                                                                                                                      |                                                                                                                                        tGGAAGATGCCTACGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCggg                                                                                                                                    <  2:570431/139‑1 (MQ=255)              cGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAcgcg                                                                                                                       >  2:91072/1‑139 (MQ=255)              cGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAcgcg                                                                                                                       <  2:828175/139‑1 (MQ=255)               gATGGCTGCGCGACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACgcgc                                                                                                                      >  2:823776/1‑139 (MQ=255)               gATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACgcgc                                                                                                                      <  1:823776/139‑1 (MQ=255)                   gCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCggg                                                                                                                                    >  2:1825387/1‑121 (MQ=255)                   gCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCggg                                                                                                                                    <  1:1825387/121‑1 (MQ=255)                    cTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGctgctg                                                                                                                 >  1:25120/1‑139 (MQ=255)                     tGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGt                                                                                                                >  1:62542/1‑139 (MQ=255)                      gcgcCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               <  2:1097476/139‑1 (MQ=255)                       ggcCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGGCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               <  1:1085485/137‑1 (MQ=255)                       cgcCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               >  2:1085485/1‑138 (MQ=255)                           aCGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCa                                                                                                          <  1:91072/139‑1 (MQ=255)                             gggTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCAcc                                                                                                        >  1:495751/1‑139 (MQ=255)                                      cTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCgctgc                                                                                                                   >  1:671875/1‑119 (MQ=255)                                      cTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCgctgc                                                                                                                   <  2:671875/119‑1 (MQ=255)                                        tCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCg                                                                                             <  2:495751/139‑1 (MQ=255)                                         cgcgAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAcg                                                                                                                         <  2:424543/110‑1 (MQ=255)                                         cgcgAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAcg                                                                                                                         >  1:424543/1‑110 (MQ=255)                                         cgcgAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAGGCGCTGCTGTTCGCCACCGGAAAATGTCGa                                                                                            >  1:1131789/1‑139 (MQ=255)                                         cgcgAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGATAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGa                                                                                            >  1:825932/1‑139 (MQ=255)                                                 aGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAGGCGCTGCTGTTCGCCACCGGAAAATGTCGATggtgcca                                                                                    <  2:1131789/139‑1 (MQ=255)                                                   ttCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGTGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               <  2:380702/110‑1 (MQ=255)                                                   ttCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               >  1:380702/1‑110 (MQ=255)                                                   ttCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCa                                                                                                          >  2:1556131/1‑115 (MQ=255)                                                   ttCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCa                                                                                                          <  1:1556131/115‑1 (MQ=255)                                                           ccGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAg                                                                          >  2:1030695/1‑139 (MQ=255)                                                           ccGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAg                                                                          >  1:37596/1‑139 (MQ=255)                                                            cgcgGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAgg                                                                         <  2:62542/139‑1 (MQ=255)                                                                   cTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCg                                                                  <  2:1068294/139‑1 (MQ=255)                                                                            gCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCa                                                         <  1:1030695/139‑1 (MQ=255)                                                                               tCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGc                                                      <  1:1264550/139‑1 (MQ=255)                                                                                      aGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTgag                                               >  2:607799/1‑139 (MQ=255)                                                                                           gTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               <  2:1231531/70‑1 (MQ=255)                                                                                           gTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                               >  1:1231531/1‑70 (MQ=255)                                                                                                      aaGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCg                                                                                             >  2:1333036/1‑77 (MQ=255)                                                                                                      aaGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCg                                                                                             <  1:1333036/77‑1 (MQ=255)                                                                                                                 tGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGc                    >  2:205259/1‑139 (MQ=255)                                                                                                                    ggTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTc                 >  2:272135/1‑139 (MQ=255)                                                                                                                     gTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCa                >  1:1055594/1‑139 (MQ=255)                                                                                                                      tAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAgg                                                       >  1:333944/1‑99 (MQ=255)                                                                                                                      tAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAgg                                                       <  2:333944/99‑1 (MQ=255)                                                                                                                      tAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCCTTAGCGGCGCAGGCTTCAc               >  1:1792361/1‑139 (MQ=255)                                                                                                                        gTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCgg                                                                 >  1:36054/1‑87 (MQ=255)                                                                                                                        gTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCgg                                                                 <  2:36054/87‑1 (MQ=255)                                                                                                                         tCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCAcgcg            >  1:1294623/1‑139 (MQ=255)                                                                                                                          cgacgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCgg           <  1:205259/139‑1 (MQ=255)                                                                                                                             cgaTAAAGCAGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGTGCAGGCTTCACGCGGAAc        >  2:1231541/1‑139 (MQ=255)                                                                                                                                tAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGcc     >  2:492635/1‑139 (MQ=255)                                                                                                                                   aGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGt  >  2:1779340/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        TGGAAGATGCCTACGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGT  >  NC_000913/4317276‑4317544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑