Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,332,869 C→T R135H (CGC→CAC)  basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,8690CT100.0% 244.9 / NA 75R135H (CGC→CAC) basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/40);  total (35/40)

ACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATG  >  NC_000913/4332734‑4333003
                                                                                                                                       |                                                                                                                                      
aCTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGAc                                                                                                                                     <  1:356447/139‑1 (MQ=255)
   aaCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGa                                                                                                                                  <  1:1398563/139‑1 (MQ=255)
    aCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGtgactgac                                                                                                                                 <  1:806622/139‑1 (MQ=255)
     cGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGAcc                                                                                                                                <  1:1569424/139‑1 (MQ=255)
        tGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACt                                                                                                                                    >  1:1215598/1‑132 (MQ=255)
        tGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACt                                                                                                                                    <  2:1215598/132‑1 (MQ=255)
         gCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGAt                                                                                                                            >  2:1305256/1‑139 (MQ=255)
               tCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTa                                                                                                                      <  1:1787600/139‑1 (MQ=255)
                  aTGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGa                                                                                                                                  <  2:735892/124‑1 (MQ=255)
                  aTGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGa                                                                                                                                  >  1:735892/1‑124 (MQ=255)
                       aTGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGaa                                                                                                              >  1:650735/1‑139 (MQ=255)
                       aTGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGaa                                                                                                              >  1:769737/1‑139 (MQ=255)
                       aTGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGaa                                                                                                              >  2:134013/1‑139 (MQ=255)
                        tGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGtgactgac                                                                                                                                 >  2:854505/1‑119 (MQ=255)
                        tGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGtgactgac                                                                                                                                 <  1:854505/119‑1 (MQ=255)
                        tGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGa                                                                                                                                      <  2:877118/114‑1 (MQ=255)
                          cGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAacc                                                                                                           >  2:919687/1‑139 (MQ=255)
                          cGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAacc                                                                                                           >  1:1651921/1‑139 (MQ=255)
                            ttttCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGa                                                                                                                                      <  2:1835919/110‑1 (MQ=255)
                            ttttCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAaccac                                                                                                         <  2:650735/139‑1 (MQ=255)
                                cGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCt                                                                                                     <  2:1488094/139‑1 (MQ=255)
                                   cagcagTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCg                                                                                                  <  1:1758874/139‑1 (MQ=255)
                                               aTGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTg                                                                                      <  2:1074385/139‑1 (MQ=255)
                                                  cAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcg                                                                                   <  2:280589/139‑1 (MQ=255)
                                                   aaaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGa                                                                                                                                      <  2:1185950/87‑1 (MQ=255)
                                                   aaaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGa                                                                                                                                      >  1:1185950/1‑87 (MQ=255)
                                                   aaaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGa                                                                                                                                  >  1:926422/1‑91 (MQ=255)
                                                   aaaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGa                                                                                                                                  <  2:926422/91‑1 (MQ=255)
                                                    aaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 <  2:769737/139‑1 (MQ=255)
                                                    aaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 <  1:1305256/139‑1 (MQ=255)
                                                    aaCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 <  2:1081653/139‑1 (MQ=255)
                                                        cACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGa                                                                             <  1:134013/139‑1 (MQ=255)
                                                         aCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 <  1:885039/134‑1 (MQ=255)
                                                         aCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 >  2:885039/1‑134 (MQ=255)
                                                           ccccGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAgg                                                                                                                             >  1:1109964/1‑87 (MQ=255)
                                                           ccccGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAgg                                                                                                                             <  2:1109964/88‑2 (MQ=255)
                                                               gCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCaa                                                                      >  1:786848/1‑139 (MQ=255)
                                                                   gTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGa                                                                                                                                      >  2:686420/1‑71 (MQ=255)
                                                                   gTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGa                                                                                                                                      <  1:686420/71‑1 (MQ=255)
                                                                     ggCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 >  1:1743417/1‑122 (MQ=255)
                                                                     ggCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 <  2:1743417/122‑1 (MQ=255)
                                                                       cGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGtt                                                              <  2:786848/139‑1 (MQ=255)
                                                                        gTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAacc                                                                                                           <  1:1465181/93‑1 (MQ=255)
                                                                        gTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAacc                                                                                                           >  2:1465181/1‑93 (MQ=255)
                                                                         ttCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTCCGTTATCCAGCGTGCTGGTCAGGTGACt                                                                                                                                    >  1:1316112/1‑67 (MQ=255)
                                                                         ttCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTCCGTTATCCAGCGTGCTGGTCAGGTGACt                                                                                                                                    <  2:1316112/67‑1 (MQ=255)
                                                                           cGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGt                                                          >  2:165544/1‑139 (MQ=255)
                                                                                ttCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTcg                                                     >  2:663877/1‑139 (MQ=255)
                                                                                 tCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTcgg                                                    <  1:919687/139‑1 (MQ=255)
                                                                                   gTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTcggcg                                                  <  1:165544/139‑1 (MQ=255)
                                                                                          aCGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 <  2:35112/101‑1 (MQ=255)
                                                                                          aCGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                                                                                 >  1:35112/1‑101 (MQ=255)
                                                                                                   gTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGt                                                               >  1:235917/1‑110 (MQ=255)
                                                                                                   gTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGt                                                               <  2:235917/110‑1 (MQ=255)
                                                                                                   gTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTgcg                                             >  2:942060/1‑128 (MQ=255)
                                                                                                   gTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTgcg                                             <  1:942060/128‑1 (MQ=255)
                                                                                                       acaacCTTTCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCttt                              >  1:724502/1‑139 (MQ=255)
                                                                                                              ttCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTa                                                                                                                      >  1:356339/1‑44 (MQ=255)
                                                                                                              ttCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTa                                                                                                                      <  2:356339/44‑1 (MQ=255)
                                                                                                              ttCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTcggcg                                                  <  1:91582/112‑1 (MQ=255)
                                                                                                              ttCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTcggcg                                                  >  2:91582/1‑112 (MQ=255)
                                                                                                               tCGTTATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCt                      <  1:595835/139‑1 (MQ=255)
                                                                                                                  ttATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTc                                                                                             <  1:944430/65‑1 (MQ=255)
                                                                                                                  ttATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTc                                                                                             >  2:944430/1‑65 (MQ=255)
                                                                                                                  ttATCCAGCGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCg                   >  2:970730/1‑139 (MQ=255)
                                                                                                                          cGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAaccac                                                                                                         >  2:1783839/1‑45 (MQ=255)
                                                                                                                          cGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAaccac                                                                                                         <  1:1783839/45‑1 (MQ=255)
                                                                                                                          cGTGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcggg           >  2:1237999/1‑139 (MQ=255)
                                                                                                                            tGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAAt                                                                     >  1:965971/1‑79 (MQ=255)
                                                                                                                            tGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAAt                                                                     <  2:965971/79‑1 (MQ=255)
                                                                                                                            tGCTGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcgggcg         >  2:549594/1‑139 (MQ=255)
                                                                                                                               tGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTc                                                                                             <  2:1707562/52‑1 (MQ=255)
                                                                                                                               tGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTc                                                                                             >  1:1707562/1‑52 (MQ=255)
                                                                                                                               tGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGt      >  2:325353/1‑139 (MQ=255)
                                                                                                                               tGGTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGt      >  2:106657/1‑139 (MQ=255)
                                                                                                                                 gTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcg                                                                                   >  2:1043324/1‑60 (MQ=255)
                                                                                                                                 gTCAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcg                                                                                   <  1:1043324/60‑1 (MQ=255)
                                                                                                                                   cAGGTGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATg  <  1:970730/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATG  >  NC_000913/4332734‑4333003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: