Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,336,405	A→G	S209S (AGT→AGC)	adiC ←	arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336,405	0	A	G	100.0%	240.6 / NA	69	S209S (AGT→AGC)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/33);  total (36/33)

GGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACGGCA  >  NC_000913/4336280‑4336537                                                                                                                              |                                                                                                                                     gggATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         >  1:495030/1‑139 (MQ=255) gggATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         >  2:1645360/1‑139 (MQ=255)                aTCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCg                                                                                                          >  1:1260020/1‑139 (MQ=255)                    cgGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAAc                                                                                                      >  2:1566040/1‑139 (MQ=255)                     ggtggtAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACg                                                                                                     >  2:1482016/1‑139 (MQ=255)                         gtAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTaa                                                                                                 >  1:1330902/1‑139 (MQ=255)                              aaGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTa                                                                                            <  2:16269/139‑1 (MQ=255)                                gTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACt                                                                                          <  1:1645360/139‑1 (MQ=255)                                 tACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTa                                                                                                        <  1:1296554/124‑1 (MQ=255)                                 tACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTa                                                                                                        >  2:1296554/1‑124 (MQ=255)                                   cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         <  2:515846/121‑1 (MQ=255)                                   cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         >  1:515846/1‑121 (MQ=255)                                   cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTg                                                                                       >  1:1689828/1‑139 (MQ=255)                                     tAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGaa                                                                                     <  2:465837/139‑1 (MQ=255)                                      aGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCAGCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCTTa                                                                                                        <  1:1063743/119‑1 (MQ=255)                                      aGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTa                                                                                                        >  2:1063743/1‑119 (MQ=255)                                      aGCAAACGGCGGCAATCAATACCCCACCAATGGAGGCGATAGGGACATTGCGTTTCGGGATTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAAt                                                                                    >  2:1053580/1‑139 (MQ=255)                                          aaCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCa                                                                                <  1:466859/139‑1 (MQ=255)                                          aaCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCa                                                                                <  2:1330902/139‑1 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         >  2:30295/1‑97 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         <  1:30295/97‑1 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGa                                                                                                                  <  2:1692378/104‑1 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGa                                                                                                                  >  1:1692378/1‑104 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGAcc                                                                                                                >  1:1303847/1‑106 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGAcc                                                                                                                <  2:1303847/106‑1 (MQ=255)                                           aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCAc                                                                               <  2:1260020/139‑1 (MQ=255)                                                              caccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                                   <  2:649084/132‑1 (MQ=255)                                                              caccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                                   >  1:649084/1‑132 (MQ=255)                                                               accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                                           >  2:1298747/1‑139 (MQ=255)                                                                 caATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         >  2:972932/1‑91 (MQ=255)                                                                 caATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAc                                                         >  2:522177/1‑139 (MQ=255)                                                                 caAAGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         <  1:972932/91‑1 (MQ=255)                                                                    tggtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGtt                                                      >  1:1321255/1‑139 (MQ=255)                                                                         gCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATg                                                 <  2:495030/139‑1 (MQ=255)                                                                          cGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                                <  2:1321255/139‑1 (MQ=255)                                                                                ggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCat                                          <  1:522177/139‑1 (MQ=255)                                                                                      tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATg                                                 >  2:1830165/1‑126 (MQ=255)                                                                                      tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATg                                                 <  1:1830165/126‑1 (MQ=255)                                                                                      tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGt                                    >  1:863870/1‑139 (MQ=255)                                                                                          tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                                           >  2:727881/1‑112 (MQ=255)                                                                                          tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                                           <  1:727881/112‑1 (MQ=255)                                                                                          tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTca                                >  1:604406/1‑139 (MQ=255)                                                                                           ttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGc                                                             <  2:1429199/109‑1 (MQ=255)                                                                                           ttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGc                                                             >  1:1429199/1‑109 (MQ=255)                                                                                            tCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAAc                                                                                                                    >  2:497005/1‑53 (MQ=255)                                                                                            tCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAAc                                                                                                                    <  1:497005/53‑1 (MQ=255)                                                                                            tCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                                <  2:205325/121‑1 (MQ=255)                                                                                            tCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                                >  1:205325/1‑121 (MQ=255)                                                                                               ggTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACg                           <  2:863870/139‑1 (MQ=255)                                                                                                gTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGc                                                                >  1:250743/1‑101 (MQ=255)                                                                                                gTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGc                                                                <  2:250743/101‑1 (MQ=255)                                                                                                  ttttCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                                   <  2:251450/96‑1 (MQ=255)                                                                                                  ttttCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                                   >  1:251450/1‑96 (MQ=255)                                                                                                   tttCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaac                       <  2:604406/139‑1 (MQ=255)                                                                                                          acacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         <  2:1609062/50‑1 (MQ=255)                                                                                                          acacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         >  1:1609062/1‑50 (MQ=255)                                                                                                           cacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCa                                                   >  2:113798/1‑103 (MQ=255)                                                                                                           cacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCa                                                   <  1:113798/103‑1 (MQ=255)                                                                                                           cacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccagaacca               >  1:1198936/1‑139 (MQ=255)                                                                                                              cTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACtt                                                                                         >  1:1004656/1‑62 (MQ=255)                                                                                                              cTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACtt                                                                                         <  2:1004656/62‑1 (MQ=255)                                                                                                                      aCGGAGGCGCTTTCCACACCGATGAACGTCCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGcc                                                                     <  1:106842/74‑1 (MQ=255)                                                                                                                      aCGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGcc                                                                     >  2:106842/1‑74 (MQ=255)                                                                                                                      aCGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacca                             >  1:714368/1‑114 (MQ=255)                                                                                                                      aCGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacca                             <  2:714368/114‑1 (MQ=255)                                                                                                                      aCGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACgg    <  1:1566040/139‑1 (MQ=255)                                                                                                                       cGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCg                                                              >  2:371049/1‑80 (MQ=255)                                                                                                                       cGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCg                                                              <  1:371049/80‑1 (MQ=255)                                                                                                                        ggAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACGGCa  >  2:428158/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                     GGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACGGCA  >  NC_000913/4336280‑4336537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑