Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,538	C→T	*213* (TAG→TAA)	msrA ←	methionine sulfoxide reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,538	0	C	T	100.0%	323.9 / NA	99	*213* (TAG→TAA)	msrA	methionine sulfoxide reductase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/50);  total (49/50)

TATGCCGATCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGCTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTG  >  NC_000913/4441404‑4441672                                                                                                                                       |                                                                                                                                       tATGCCGATCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATg                                                                                                                                    >  1:779643/1‑139 (MQ=255)        aTCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCcgg                                                                                                                             >  2:1174061/1‑139 (MQ=255)                       tCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgcc                                                                                                              >  1:638147/1‑139 (MQ=255)                       tCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgcc                                                                                                              >  2:303106/1‑139 (MQ=255)                                 atatTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                      <  1:1495078/137‑1 (MQ=255)                                 atatTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                      >  2:1495078/1‑137 (MQ=255)                                   atTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcagaca                                                                                                                    <  1:1736839/121‑1 (MQ=255)                                   atTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcagaca                                                                                                                    >  2:1736839/1‑121 (MQ=255)                                       aaTTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaattc                                                                                                >  2:1362838/1‑137 (MQ=255)                                       aaTTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaattc                                                                                                <  1:1362838/137‑1 (MQ=255)                                       aaTTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACgccaatgccgccaactccc                                                                                              <  1:141475/139‑2 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaa                                                                                                   <  1:141308/129‑1 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaa                                                                                                   >  2:141308/1‑129 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                      <  1:1569330/126‑1 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                      >  2:1569330/1‑126 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         >  1:283944/1‑139 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         >  1:295987/1‑139 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         >  2:984972/1‑139 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         >  2:55854/1‑139 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         >  1:141525/1‑139 (MQ=255)                                                    ttGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                                 <  2:141525/139‑1 (MQ=255)                                                             tGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        <  2:283944/139‑1 (MQ=255)                                                             tGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        <  1:1174061/139‑1 (MQ=255)                                                              ggTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATg                                                                       <  1:55854/139‑1 (MQ=255)                                                               gTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACATACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGc                                                                      >  1:912710/1‑139 (MQ=255)                                                               gTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcagaca                                                                                                                    >  2:979387/1‑93 (MQ=255)                                                               gTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcagaca                                                                                                                    <  1:979387/93‑1 (MQ=255)                                                               gTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGc                                                                      >  1:1430489/1‑139 (MQ=255)                                                                       tttACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGtt                                                                             <  2:1474793/124‑1 (MQ=255)                                                                       tttACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGtt                                                                             >  1:1474793/1‑124 (MQ=255)                                                                        ttACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGc                                                             <  2:1430489/139‑1 (MQ=255)                                                                              cgcATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                       >  2:1423322/1‑139 (MQ=255)                                                                                cATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTc                                                     >  1:1125080/1‑139 (MQ=255)                                                                                cATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTc                                                     >  1:1084823/1‑139 (MQ=255)                                                                                   cAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATc                                                  <  1:1423322/139‑1 (MQ=255)                                                                                        aTAGACAAAGAGCAGTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGTCAGACGCCAATTCCGCCAATTGCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                             <  2:1125080/139‑1 (MQ=255)                                                                                        aTAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                             <  2:1084823/139‑1 (MQ=255)                                                                                        aTAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                             <  1:928178/139‑1 (MQ=255)                                                                                          aGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaatt                                                                                                 >  2:977519/1‑85 (MQ=255)                                                                                          aGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaatt                                                                                                 <  1:977519/85‑1 (MQ=255)                                                                                          aGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCa                                           <  2:295987/139‑1 (MQ=255)                                                                                           gACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCat                                          >  1:662394/1‑139 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCcaca                                                                                            <  2:1438705/84‑1 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCcaca                                                                                            >  1:1438705/1‑84 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                                 >  1:1584655/1‑95 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                                 <  2:1584655/95‑1 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        >  2:1017913/1‑104 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        <  1:1017913/104‑1 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                       <  2:1511993/121‑1 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                       >  1:1511993/1‑121 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCAtaataa                                     >  1:1306731/1‑139 (MQ=255)                                                                                                  agCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         >  1:1503688/1‑85 (MQ=255)                                                                                                  agCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTa                                                                                         <  2:1503688/85‑1 (MQ=255)                                                                                                  agCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATaaaa                                   <  1:676892/139‑1 (MQ=255)                                                                                                  agCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATaaaa                                   >  1:1041853/1‑139 (MQ=255)                                                                                                      cTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCca                                                                                              <  2:1537160/76‑1 (MQ=255)                                                                                                      cTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCca                                                                                              >  1:1537160/1‑76 (MQ=255)                                                                                                       tttATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGgtg                              <  2:638147/139‑1 (MQ=255)                                                                                                        ttATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGgtgt                             <  2:1306731/139‑1 (MQ=255)                                                                                                            caacaaACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCa                                           <  2:1812644/121‑1 (MQ=255)                                                                                                            caacaaACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCa                                           >  1:1812644/1‑121 (MQ=255)                                                                                                               caaACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                     >  2:1723648/1‑92 (MQ=255)                                                                                                               caaACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                     <  1:1723648/92‑1 (MQ=255)                                                                                                                 caCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGGGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGc                    >  1:373766/2‑139 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                     >  1:324894/1‑90 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                     <  2:324894/90‑1 (MQ=255)                                                                                                                    tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt                 <  2:1041853/139‑1 (MQ=255)                                                                                                                    tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt                 <  1:1401053/139‑1 (MQ=255)                                                                                                                      cATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAttt               <  1:984972/139‑1 (MQ=255)                                                                                                                       aTTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCtt                                                >  2:26094/1‑105 (MQ=255)                                                                                                                       aTTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCtt                                                <  1:26094/105‑1 (MQ=255)                                                                                                                         ttGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCg            <  2:373766/139‑1 (MQ=255)                                                                                                                         ttGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCg            <  1:303106/139‑1 (MQ=255)                                                                                                                            tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATg                                                                       >  1:543014/1‑77 (MQ=255)                                                                                                                            tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATg                                                                       <  2:543014/77‑1 (MQ=255)                                                                                                                            tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATaaaa                                   >  2:1219161/1‑113 (MQ=255)                                                                                                                            tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATaaaa                                   <  1:1219161/113‑1 (MQ=255)                                                                                                                             aCCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTggtggt                                                      >  1:319621/1‑93 (MQ=255)                                                                                                                             aCCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTggtggt                                                      <  2:319621/93‑1 (MQ=255)                                                                                                                             aCCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCgtggt        <  2:662394/139‑1 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGata                                                                 >  2:1801039/1‑81 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGata                                                                 <  1:1801039/81‑1 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                       >  1:718217/1‑91 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                       <  2:718217/91‑1 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTc                                                     <  1:1662189/93‑1 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTc                                                     >  2:1662189/1‑93 (MQ=255)                                                                                                                                cGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                                 <  1:144355/63‑1 (MQ=255)                                                                                                                                cGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                                 >  2:144355/1‑63 (MQ=255)                                                                                                                                cGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGat                                                                  <  1:1501723/78‑1 (MQ=255)                                                                                                                                cGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGat                                                                  >  2:1501723/1‑78 (MQ=255)                                                                                                                                 gTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGtttt                                                                           <  2:1516611/68‑1 (MQ=255)                                                                                                                                 gTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGtttt                                                                           >  1:1516611/1‑68 (MQ=255)                                                                                                                                 gTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCa                                           <  1:1757110/100‑1 (MQ=255)                                                                                                                                 gTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCa                                           >  2:1757110/1‑100 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                      <  1:808511/39‑1 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                      >  2:808511/1‑39 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        <  2:1810300/69‑1 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        >  1:1810300/1‑69 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGAtgtg  >  2:1222867/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TATGCCGATCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGCTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTG  >  NC_000913/4441404‑4441672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑