Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,519,014 G→A pseudogene (294/504 nt) insB1 → IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,0140GA98.2% 303.4 / ‑6.6 111pseudogene (294/504 nt)insB1IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (58/51);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGA  >  NC_000913/4518892‑4519144
                                                                                                                          |                                                                                                                                  
tggctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    <  1:1327718/139‑1 (MQ=255)
 ggctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                                   <  2:825523/139‑1 (MQ=255)
  gctgTTTTACGCGTATGACAGGGTACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGGCTGATGGCTGGCCg                                                                                                                  <  1:804841/139‑1 (MQ=255)
  gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  <  2:1385737/139‑1 (MQ=255)
                aTGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACg                                                                                                    >  1:1351862/1‑139 (MQ=255)
                            ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGa                                                                                                                            <  1:1426072/103‑1 (MQ=255)
                            ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGa                                                                                                                            >  2:1426072/1‑103 (MQ=255)
                            ggaggaCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTg                                                                                                >  1:1127529/1‑131 (MQ=255)
                            ggaggaCGGATGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGCTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTg                                                                                                <  2:1127529/131‑1 (MQ=255)
                            ggaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                                   >  1:258125/5‑111 (MQ=11)
                            ggaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                                   <  2:258125/107‑1 (MQ=11)
                                 aCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                                  >  1:921273/1‑139 (MQ=14)
                                   ggTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTa                                                                                                             <  1:1256780/110‑1 (MQ=14)
                                     ttgttgCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAg                                                                                       <  1:1091741/130‑1 (MQ=14)
                                     ttgttgCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAg                                                                                       >  2:1091741/1‑130 (MQ=14)
                                     ttGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  <  2:1020048/104‑1 (MQ=255)
                                     ttGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  >  1:1020048/1‑104 (MQ=255)
                                        ttgcgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATc                                                                           >  1:884924/3‑139 (MQ=14)
                                        tgGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTAt                                                                            >  2:178027/1‑139 (MQ=255)
                                        tgGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTAt                                                                            >  2:1044022/1‑139 (MQ=255)
                                           cgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                                       <  2:1469127/92‑1 (MQ=17)
                                           cgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                                       >  1:1469127/1‑92 (MQ=14)
                                             cACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                                                      <  2:1590311/108‑1 (MQ=255)
                                             cACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                                                      >  1:1590311/1‑108 (MQ=255)
                                             cACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCa                                                                       >  1:1012315/1‑139 (MQ=255)
                                              aCGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                                      >  1:1078298/1‑139 (MQ=255)
                                               cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  >  1:122659/1‑94 (MQ=255)
                                               cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  <  2:122659/94‑1 (MQ=255)
                                               cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGtt                                                                              <  2:548984/130‑1 (MQ=255)
                                               cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGtt                                                                              >  1:548984/1‑130 (MQ=255)
                                               cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAg                                                                     >  1:994326/1‑139 (MQ=255)
                                               cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAg                                                                     >  1:761214/1‑139 (MQ=255)
                                               cGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                                   >  2:1727848/1‑92 (MQ=17)
                                                   ttCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                                       >  2:740158/1‑84 (MQ=17)
                                                       gTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTacac                                                             <  2:1012315/139‑1 (MQ=255)
                                                          aaCGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggc                                                                                                                        <  2:481976/76‑1 (MQ=18)
                                                          aaCGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggc                                                                                                                        >  1:481976/1‑76 (MQ=18)
                                                            cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAgc                                                        >  2:146944/1‑139 (MQ=255)
                                                            cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCGGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAgc                                                        >  1:185977/1‑139 (MQ=255)
                                                               aCTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCa                                                     >  1:787026/1‑139 (MQ=255)
                                                                 tctGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCAtt                                                   >  1:56546/1‑139 (MQ=255)
                                                                  ctGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTg                                                  >  1:290610/1‑139 (MQ=255)
                                                                  atggcgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                    >  2:369900/10‑104 (MQ=18)
                                                                  atGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                    <  1:369900/103‑1 (MQ=18)
                                                                   tGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGa                                                 <  2:761214/139‑1 (MQ=255)
                                                                    ggcgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    <  1:597660/63‑1 (MQ=21)
                                                                    ggcgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    >  2:597660/8‑70 (MQ=21)
                                                                    ggcgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                                                <  2:407767/131‑1 (MQ=11)
                                                                    ggCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                                                >  1:407767/1‑138 (MQ=11)
                                                                      cgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCgg                                             >  1:359819/6‑138 (MQ=11)
                                                                         cacTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACa                                           <  1:417787/139‑1 (MQ=255)
                                                                           cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                                      <  2:1128529/109‑1 (MQ=18)
                                                                           cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                                      >  1:1128529/1‑109 (MQ=18)
                                                                           cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                                                 >  2:136617/1‑130 (MQ=14)
                                                                           cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                                                 <  1:136617/130‑1 (MQ=14)
                                                                           cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggtataa                                        >  1:1106433/1‑133 (MQ=11)
                                                                             ggAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaat                                       <  1:146944/139‑1 (MQ=255)
                                                                             ggAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                                       <  1:1691200/58‑1 (MQ=255)
                                                                             ggAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                                       >  2:1691200/1‑58 (MQ=255)
                                                                               aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatct                                     >  1:244993/1‑139 (MQ=255)
                                                                               aGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGTATaacctg                                    >  1:1762587/1‑139 (MQ=11)
                                                                                   tcttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGCAGGGAAAGCTGCACGTTATCAGCCAGCGTTACACTCAGCGCATTGAGCGACATaatctgaat                                 >  1:531386/1‑139 (MQ=255)
                                                                                   tcttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatctgaat                                 <  2:244993/139‑1 (MQ=255)
                                                                                    cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTc                                                           >  2:742543/1‑112 (MQ=255)
                                                                                    cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTc                                                           <  1:742543/112‑1 (MQ=255)
                                                                                    cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatctgaatc                                >  2:344542/1‑139 (MQ=255)
                                                                                          aGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGac                          <  1:139914/139‑1 (MQ=255)
                                                                                              tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGacaaca                      >  2:397652/1‑139 (MQ=255)
                                                                                              tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGacaaca                      <  2:1351862/139‑1 (MQ=255)
                                                                                              tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGacaaca                      >  1:12376/1‑139 (MQ=255)
                                                                                              tgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGacaaca                      >  1:1671985/1‑139 (MQ=255)
                                                                                              tgctgTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                                                          >  2:1015048/1‑70 (MQ=21)
                                                                                              tgctgTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                                                          <  1:1015048/70‑1 (MQ=21)
                                                                                              tgctgTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAgcgaa                                                     >  2:1466624/1‑105 (MQ=11)
                                                                                              tgctgTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAgcgaa                                                     <  1:1466624/107‑3 (MQ=11)
                                                                                               gctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    >  2:1308760/1‑44 (MQ=255)
                                                                                               gctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    <  1:1308760/44‑1 (MQ=255)
                                                                                               gctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACa                                           >  2:1443558/1‑117 (MQ=255)
                                                                                                ctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                    >  1:1644924/1‑75 (MQ=255)
                                                                                                ctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                    <  2:1644924/75‑1 (MQ=255)
                                                                                                 tgTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    <  1:724842/42‑1 (MQ=255)
                                                                                                 tgTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                    >  2:724842/1‑42 (MQ=255)
                                                                                                 ctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                                      <  1:1516948/84‑1 (MQ=17)
                                                                                                 ctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                                                 <  1:1005087/105‑1 (MQ=11)
                                                                                                  tgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  <  2:858111/40‑1 (MQ=25)
                                                                                                  tgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                  >  1:858111/4‑43 (MQ=25)
                                                                                                   tCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTgg                 <  2:1157378/139‑1 (MQ=255)
                                                                                                         tttGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGc           >  2:274621/1‑139 (MQ=255)
                                                                                                         tttGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGc           <  2:450475/139‑1 (MQ=255)
                                                                                                           tGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTa                                                                                                             >  1:65314/1‑39 (MQ=21)
                                                                                                           tGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATc                                                                           >  2:370742/1‑73 (MQ=17)
                                                                                                             aGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTggg       <  1:1044022/139‑1 (MQ=255)
                                                                                                             aGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTggg       <  2:787026/139‑1 (MQ=255)
                                                                                                             aGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTggg       <  1:178027/139‑1 (MQ=255)
                                                                                                                 acaggTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGGGAACAGCAAGAGTTACACCCAGCGCATTGAGCGACATAAGCTGAATCTGAGACAACATCTGGCAAGGCTGGGACgg   <  2:803906/136‑1 (MQ=18)
                                                                                                                  gTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                 >  2:1669125/1‑44 (MQ=255)
                                                                                                                  gTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                 <  1:1669125/44‑1 (MQ=255)
                                                                                                                  gTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGa  <  1:274621/139‑1 (MQ=255)
                                                                                                                   tGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGtt                                                                              <  1:753222/62‑1 (MQ=255)
                                                                                                                   tGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGtt                                                                              >  2:753222/1‑62 (MQ=255)
                                                                                                                          |                                                                                                                                  
TGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGA  >  NC_000913/4518892‑4519144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: