Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,549,001 G→A C65Y (TGC→TAC)  fimH → minor component of type 1 fimbriae

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,0010GA100.0% 251.5 / NA 77C65Y (TGC→TAC) fimHminor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/42);  total (35/42)

CGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCAC  >  NC_000913/4548873‑4549130
                                                                                                                                |                                                                                                                                 
cGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGAt                                                                                                                         <  2:1278598/139‑1 (MQ=255)
      tAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCg                                                                                                                   <  2:1086358/139‑1 (MQ=255)
          aCCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGaaa                                                                                                               <  2:235855/139‑1 (MQ=255)
                    gTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTGGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGa                                                                                                     <  1:1467964/139‑1 (MQ=255)
                         gCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATg                                                                                                <  1:714999/139‑1 (MQ=255)
                         gCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATg                                                                                                <  2:1786568/139‑1 (MQ=255)
                             tCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTcac                                                                                            >  2:243448/1‑139 (MQ=255)
                              cccTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTcaca                                                                                           >  1:1081732/1‑139 (MQ=255)
                              cccTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTcaca                                                                                           >  2:310801/1‑139 (MQ=255)
                               ccTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCAt                                                                                                                               <  1:1111805/102‑1 (MQ=255)
                               ccTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCAt                                                                                                                               >  2:1111805/1‑102 (MQ=255)
                                      gCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACg                                                                                   <  1:310801/139‑1 (MQ=255)
                                        ggTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGa                                                                                                                          >  1:1134899/1‑98 (MQ=255)
                                        ggTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGa                                                                                                                          <  2:1134899/98‑1 (MQ=255)
                                         gTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACa                                                                                                       <  2:778688/116‑1 (MQ=255)
                                         gTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACa                                                                                                       >  1:778688/1‑116 (MQ=255)
                                           ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTa                                                                                                         <  2:1658291/112‑1 (MQ=255)
                                           ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTa                                                                                                         >  1:1658291/1‑112 (MQ=255)
                                           ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTca                                                                                             >  1:83218/1‑124 (MQ=255)
                                           ggCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTca                                                                                             <  2:83218/124‑1 (MQ=255)
                                                      tGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACa                                                                                                       <  2:345091/103‑1 (MQ=255)
                                                      tGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACa                                                                                                       >  1:345091/1‑103 (MQ=255)
                                                       gTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                                  <  1:1334387/139‑1 (MQ=255)
                                                         ttATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcggc                                                                <  2:1081732/139‑1 (MQ=255)
                                                            tGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgtg                                                             <  2:1295397/139‑1 (MQ=255)
                                                                 aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATc                                                        >  2:1313921/1‑139 (MQ=255)
                                                                 aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATc                                                        >  1:1428878/1‑139 (MQ=255)
                                                                     tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATg                                                                                                >  1:448656/1‑95 (MQ=255)
                                                                     tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATg                                                                                                <  2:448656/95‑1 (MQ=255)
                                                                      gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACg                                                                                   <  1:1073319/107‑1 (MQ=255)
                                                                      gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACg                                                                                   >  2:1073319/1‑107 (MQ=255)
                                                                      gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAtt                                                   >  2:1138675/1‑139 (MQ=255)
                                                                             tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCTGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                                            <  2:557112/139‑1 (MQ=255)
                                                                                  aaTGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCg                                       <  1:1138675/139‑1 (MQ=255)
                                                                                    tgtgGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                                     <  1:12666/139‑1 (MQ=255)
                                                                                    tgtgGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCGTTTACCATAACGATTATCCGGAAGCCAGTACAGACTATGTCACACTGCAACGAGGCTCGGCGTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                                     <  1:1677104/139‑1 (MQ=255)
                                                                                      tgGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                                  >  1:676708/1‑108 (MQ=255)
                                                                                      tgGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                                  <  2:676708/108‑1 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCa                                                                                                                                <  2:1010034/44‑1 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCa                                                                                                                                >  1:1010034/1‑44 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGAt                                                                                                                         >  2:173619/1‑51 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGAt                                                                                                                         <  1:173619/51‑1 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTcac                                                                                            >  2:974618/1‑80 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTcac                                                                                            <  1:974618/80‑1 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                                              >  2:610285/1‑94 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                                              <  1:610285/94‑1 (MQ=255)
                                                                                        gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat                                 >  1:565158/1‑139 (MQ=255)
                                                                                         ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATaa                                                                                                                             <  1:1626040/46‑1 (MQ=255)
                                                                                         ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATaa                                                                                                                             >  2:1626040/1‑46 (MQ=255)
                                                                                              aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTcg                                                                                             <  1:1144738/73‑2 (MQ=255)
                                                                                              aaCCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTca                                                                                             >  2:1144738/1‑73 (MQ=255)
                                                                                               aCCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGaaa                                                                                                               <  1:676699/54‑1 (MQ=255)
                                                                                               aCCTGGTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGaaa                                                                                                               >  2:676699/1‑54 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCg                                                                                                                   >  1:670568/1‑46 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCg                                                                                                                   <  2:670568/46‑1 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTa                                                                                                         <  1:1727470/56‑1 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTa                                                                                                         >  2:1727470/1‑56 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTca                                                                                             >  1:656790/1‑68 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTca                                                                                             <  2:656790/68‑1 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgtg                                                             >  1:296881/1‑100 (MQ=255)
                                                                                                   ggTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCgtg                                                             <  2:296881/100‑1 (MQ=255)
                                                                                                    gTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCg                                                                            <  1:80780/84‑1 (MQ=255)
                                                                                                    gTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCg                                                                            >  2:80780/1‑84 (MQ=255)
                                                                                                    gTCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTa                     >  1:1335999/1‑139 (MQ=255)
                                                                                                     tCGTGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                    <  2:1335999/139‑1 (MQ=255)
                                                                                                        tGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                          >  2:131127/1‑114 (MQ=255)
                                                                                                        tGGATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                          <  1:131127/114‑1 (MQ=255)
                                                                                                         ggATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTAt                <  1:1641279/139‑1 (MQ=255)
                                                                                                          gATCTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATc               >  1:1425267/1‑139 (MQ=255)
                                                                                                             cTTTCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTCCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAt            <  1:1313921/139‑1 (MQ=255)
                                                                                                              tttCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAAtata                              <  1:1598823/120‑1 (MQ=255)
                                                                                                              tttCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAAtata                              >  2:1598823/1‑120 (MQ=255)
                                                                                                               ttCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAttt          >  2:1688910/1‑139 (MQ=255)
                                                                                                               ttCGACGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAttt          >  2:1206590/1‑139 (MQ=255)
                                                                                                                   aCGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTa                                                                       <  2:132555/74‑1 (MQ=255)
                                                                                                                   aCGCAAATCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTa                                                                       >  1:132555/1‑74 (MQ=255)
                                                                                                                       aaaTCTTTTACCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTaccac  <  1:1688910/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                 
CGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCAC  >  NC_000913/4548873‑4549130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑