Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,577,958	C→T	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC ← mcrB ←	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,577,958	0	C	T	99.0%	305.5 / ‑4.6	96	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC mcrB	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (51/44);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

ATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCA  >  NC_000913/4577827‑4578090                                                                                                                                    |                                                                                                                                     atacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                  >  2:82898/1‑136 (MQ=255) atacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                  <  1:82898/136‑1 (MQ=255)  tacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGAGAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                              >  2:55132/1‑139 (MQ=255)  tacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                              <  2:1154006/139‑1 (MQ=255)   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                             >  1:1766578/1‑139 (MQ=255)   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                             >  2:565605/1‑139 (MQ=255)   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTcc                                                                                                                             >  2:654931/1‑139 (MQ=255)        aGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaa                                                                                                                        >  1:1234931/1‑139 (MQ=255)        aGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaa                                                                                                                        >  1:765946/1‑139 (MQ=255)                        tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                <  2:480032/115‑1 (MQ=255)                        tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                >  1:480032/1‑115 (MQ=255)                        tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                        <  1:682141/139‑1 (MQ=255)                        tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                        <  1:565605/139‑1 (MQ=255)                             ccGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   <  2:458342/139‑1 (MQ=255)                                          cAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTGCGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAgggg                                                                                      >  2:743196/1‑139 (MQ=255)                                          cAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAggg                                                                                       >  1:1119943/1‑138 (MQ=255)                                          cAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTCTATAggg                                                                                       <  2:1119943/138‑1 (MQ=255)                                           aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                >  2:1560386/1‑96 (MQ=255)                                           aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                <  1:1560386/96‑1 (MQ=255)                                           aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGt                                                                                     >  2:444316/1‑139 (MQ=255)                                              gTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                  <  2:1234931/139‑1 (MQ=255)                                                ttGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGAGAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                <  1:55132/139‑1 (MQ=255)                                                         tAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGCTTATAGGGGTCATCAAAGAAatat                                                                       >  2:1024826/1‑139 (MQ=255)                                                                 tGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                >  1:183695/1‑74 (MQ=255)                                                                 tGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                <  2:183695/74‑1 (MQ=255)                                                                     aaTAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTaaa                                                           <  2:765946/139‑1 (MQ=255)                                                                        aaCCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                >  2:652421/1‑67 (MQ=255)                                                                        aaCCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                <  1:652421/67‑1 (MQ=255)                                                                          ccccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                        <  1:301919/89‑1 (MQ=255)                                                                          ccccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                        >  2:301919/1‑89 (MQ=255)                                                                          ccccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGc                                                      >  1:1577640/1‑139 (MQ=255)                                                                          ccccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGc                                                      >  1:1667723/1‑139 (MQ=255)                                                                              aTGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGata                                                  <  2:1577640/139‑1 (MQ=255)                                                                               tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                  <  2:322914/106‑1 (MQ=255)                                                                               tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                  >  1:322914/1‑106 (MQ=255)                                                                               tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGatat                                                 >  2:1733501/1‑139 (MQ=255)                                                                                gCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATc                                                <  1:444316/139‑1 (MQ=255)                                                                                     ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                              <  1:1755317/56‑1 (MQ=255)                                                                                     ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                              >  2:1755317/1‑56 (MQ=255)                                                                                     ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                       <  1:1698688/127‑1 (MQ=255)                                                                                     ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                       >  2:1698688/1‑127 (MQ=255)                                                                                      gTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcat                                          >  1:144242/1‑139 (MQ=255)                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTa                                                             <  1:600551/117‑1 (MQ=255)                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTa                                                             >  2:600551/1‑117 (MQ=255)                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATcac                                       >  1:1036019/1‑139 (MQ=255)                                                                                           cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTaaa                                                           <  2:360097/117‑1 (MQ=255)                                                                                           cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTaaa                                                           >  1:360097/1‑117 (MQ=255)                                                                                           cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACaa                                     >  2:360741/1‑139 (MQ=255)                                                                                            aTGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                    <  2:1766578/139‑1 (MQ=255)                                                                                                aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCa                                >  2:511789/1‑139 (MQ=255)                                                                                                   aGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTa                             <  1:360741/139‑1 (MQ=255)                                                                                                       atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcatca                                        >  1:1046851/1‑124 (MQ=255)                                                                                                       atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcatca                                        <  2:1046851/124‑1 (MQ=255)                                                                                                       atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGcc                         <  1:875325/139‑1 (MQ=255)                                                                                                       atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGcc                         >  2:960515/1‑139 (MQ=255)                                                                                                       atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGcc                         >  2:875325/1‑139 (MQ=255)                                                                                                        ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCa                        >  2:1726181/1‑139 (MQ=255)                                                                                                         tACGGACAGGTATCACGGGCTGTTCCGTTATGAGTCCACTAATATTTTGTTGGTCAATTTCTGTCGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGGAAAGGGGCGATATCCGTCATCACACTTTCATTAAGcaaa                       >  1:1211498/1‑136 (MQ=255)                                                                                                         tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   >  2:766686/1‑63 (MQ=255)                                                                                                         tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   <  1:766686/63‑1 (MQ=255)                                                                                                         tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCAt                       >  2:399284/1‑139 (MQ=255)                                                                                                              aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   <  2:1686940/58‑1 (MQ=255)                                                                                                              aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   >  1:1686940/1‑58 (MQ=255)                                                                                                                aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                    <  2:1457350/119‑1 (MQ=255)                                                                                                                aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                    >  1:1457350/1‑119 (MQ=255)                                                                                                                 ggTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATc               >  1:1062975/1‑139 (MQ=255)                                                                                                                  gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCg                   <  1:933299/134‑1 (MQ=255)                                                                                                                  gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATcc              >  1:1072394/1‑139 (MQ=255)                                                                                                                  gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTAGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACACTTTCATTAAGCCATTGCg                   >  2:933299/1‑134 (MQ=255)                                                                                                                   tATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCg             <  2:1062975/139‑1 (MQ=255)                                                                                                                          ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                 <  2:1403927/48‑1 (MQ=255)                                                                                                                          ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                 >  1:1403927/1‑48 (MQ=255)                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   <  1:1813024/44‑1 (MQ=255)                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   >  2:1813024/1‑44 (MQ=255)                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTTGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                    >  1:1310774/1‑123 (MQ=255)                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                       <  2:781808/88‑1 (MQ=255)                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                       >  1:781808/1‑88 (MQ=255)                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                    <  2:1310774/123‑1 (MQ=255)                                                                                                                             tGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAggg                                                                                       <  2:439570/55‑1 (MQ=255)                                                                                                                             tGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAggg                                                                                       >  1:439570/1‑55 (MQ=255)                                                                                                                              gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAg                                                                                         <  2:540463/52‑1 (MQ=255)                                                                                                                              gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAg                                                                                         >  1:540463/1‑52 (MQ=255)                                                                                                                              gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTg                     >  2:1537363/1‑120 (MQ=255)                                                                                                                              gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTg                     <  1:1537363/120‑1 (MQ=255)                                                                                                                              gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCa  <  2:1036019/139‑1 (MQ=255)                                                                                                                              gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCa  <  1:511789/139‑1 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   >  1:1783964/1‑41 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                   <  2:1783964/41‑1 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAg                                                                             <  2:288220/63‑1 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAg                                                                             >  1:288220/1‑63 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttct                                                                    >  1:1606396/1‑72 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttct                                                                    <  2:1606396/72‑1 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCAtt                              >  1:46348/1‑110 (MQ=255)                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCAtt                              <  2:46348/110‑1 (MQ=255)                                                                                                                                tCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGt                  >  1:985069/1‑121 (MQ=255)                                                                                                                                tCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGt                  <  2:985069/121‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     ATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCA  >  NC_000913/4577827‑4578090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑