Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,239	A→G	noncoding (48/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,239	0	A	G	100.0%	205.4 / NA	60	noncoding (48/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACC  >  NC_000913/4606135‑4606365                                                                                                          |                                                                                                                               cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                                           <  1:1076545/111‑1 (MQ=255) cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                                           >  2:1076545/1‑111 (MQ=255)   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGACGCTAGCGCCTCTAACAATTc                                                                                             >  1:1764510/1‑139 (MQ=25)   aGCGCGTCTACCAATTCCGCCACCTTCGCACACTCTTATTACTTTTTTTGATATCGCCGCGCTTGGTGGGATGGGGGGGGTCTTCAGCCTCCACGCCCGAATGAACACTCAACCCAGAACCTAGTCCGGCAACCAATTc                                                                                             >  1:1358669/1‑139 (MQ=255)     cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                                         <  1:592639/109‑1 (MQ=255)     cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                                         >  2:592639/1‑109 (MQ=255)     cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                                            <  2:1747450/122‑1 (MQ=255)     cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                                            >  1:1747450/1‑122 (MQ=255)      gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCAAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                          <  1:876829/139‑1 (MQ=255)          cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                      >  2:191541/1‑139 (MQ=255)                aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                <  2:1682778/139‑1 (MQ=255)                     gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGTGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                           >  2:1411646/1‑139 (MQ=255)                     gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                           >  1:307471/1‑139 (MQ=255)                          cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc                                                                      >  2:390823/1‑139 (MQ=255)                            tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa                                                                    >  1:135447/1‑139 (MQ=255)                            tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa                                                                    >  1:555417/1‑139 (MQ=255)                            tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCCCCCAGTGGGAGCaa                                                                    >  1:566217/1‑139 (MQ=18)                                   gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGTGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                             <  1:1411646/139‑1 (MQ=255)                                   gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                             <  2:135447/139‑1 (MQ=255)                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                <  1:601730/119‑1 (MQ=255)                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                >  2:601730/1‑119 (MQ=255)                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACg                                                            >  2:834416/1‑139 (MQ=255)                                     cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                       <  2:1811731/127‑1 (MQ=255)                                     cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                       >  1:1811731/1‑127 (MQ=255)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                                                     >  1:1668171/1‑88 (MQ=17)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                                                     <  2:1668171/88‑1 (MQ=17)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                  >  2:464654/1‑91 (MQ=255)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                  <  1:464654/91‑1 (MQ=17)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                            <  2:1327656/113‑1 (MQ=255)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                            >  1:1327656/1‑113 (MQ=255)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                         <  1:700491/116‑1 (MQ=255)                                              ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                         >  2:700491/1‑116 (MQ=255)                                                ttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                <  1:1302845/107‑1 (MQ=255)                                                ttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                >  2:1302845/1‑107 (MQ=255)                                                  ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTACGAGCAATAAAAACGTGGTTTTTGGtccca                                             >  1:1320142/1‑135 (MQ=18)                                                       atCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                         >  1:1747094/1‑139 (MQ=255)                                                               gTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt                                 <  1:390823/139‑1 (MQ=255)                                                                tttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTg                                >  2:593010/1‑139 (MQ=255)                                                                  tGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa                              >  2:1313263/1‑139 (MQ=255)                                                                     tGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                             <  1:123421/105‑1 (MQ=255)                                                                     tGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                             >  2:123421/1‑105 (MQ=255)                                                                      gcgagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTTTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                          <  1:1313263/134‑1 (MQ=255)                                                                      gcgagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                          <  2:555417/134‑1 (MQ=255)                                                                        gagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                        <  2:1764510/136‑1 (MQ=255)                                                                        gagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                        <  2:307471/136‑1 (MQ=255)                                                                         agggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACTCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCAc                       >  1:739073/3‑139 (MQ=255)                                                                          gggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                      <  2:739073/138‑1 (MQ=255)                                                                               ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                             <  2:1709080/95‑1 (MQ=255)                                                                               ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                             >  1:1709080/1‑95 (MQ=255)                                                                                gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                <  2:1747094/139‑1 (MQ=255)                                                                                      tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca          <  1:605901/139‑1 (MQ=255)                                                                                        aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt        <  1:191541/139‑1 (MQ=255)                                                                                          cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa      >  1:756031/1‑139 (MQ=255)                                                                                          cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa      <  2:756031/139‑1 (MQ=255)                                                                                          cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGAGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa      <  2:680334/139‑1 (MQ=255)                                                                                             ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac   >  2:605901/1‑139 (MQ=255)                                                                                              cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc  <  2:566217/139‑1 (MQ=255)                                                                                               aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                                <  2:1240383/108‑1 (MQ=255)                                                                                               aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                          >  1:1730384/1‑114 (MQ=255)                                                                                               aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                          <  2:1730384/114‑1 (MQ=255)                                                                                               aCGTCCGTAAGGACACTAACACCTAAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                                >  1:1240383/1‑108 (MQ=255)                                                                                                          |                                                                                                                               CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACC  >  NC_000913/4606135‑4606365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑