Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,260	G→A	*260* (TAG→TAA)	yjjV →	putative DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,260	0	G	A	100.0%	157.4 / NA	49	*260* (TAG→TAA)	yjjV	putative DNase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/24);  total (25/24)

GGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGT  >  NC_000913/4614131‑4614394                                                                                                                                  |                                                                                                                                       ggggCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATaa                                                                                                                               <  2:1620509/139‑1 (MQ=255)     cAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCg                                                                                                                           >  2:344831/1‑139 (MQ=255)       gCCTAACCGCCCGGTGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGtt                                                                                                                         >  1:64275/1‑139 (MQ=255)       gCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGtt                                                                                                                         >  2:291765/1‑139 (MQ=255)             ccGCCCGGTGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcgcc                                                                                                                   <  2:64275/139‑1 (MQ=255)             ccGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcgcc                                                                                                                   <  1:291765/139‑1 (MQ=255)                          gCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt                                                                                                      >  2:1515992/1‑139 (MQ=255)                               tcGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcgc                                                                                                 <  2:1480312/138‑1 (MQ=255)                                  tgtgtTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcaca                                                                                              <  1:1515992/139‑1 (MQ=255)                                        cGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCt                                                                                        >  1:1034765/1‑139 (MQ=255)                                        cGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCt                                                                                        >  1:540363/1‑139 (MQ=255)                                        cGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCt                                                                                        >  2:638793/1‑139 (MQ=255)                                                    cGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                                            >  2:337758/1‑139 (MQ=255)                                                       gTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc                                                                         <  1:1723799/139‑1 (MQ=255)                                                          gcgcCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcg                                                                                                                     <  1:1590717/92‑1 (MQ=255)                                                          gcgcCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcg                                                                                                                     >  2:1590717/1‑92 (MQ=255)                                                                ggAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTAt                                                                >  1:472986/1‑139 (MQ=255)                                                                 gAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATc                                                               <  2:540363/139‑1 (MQ=255)                                                                        cggATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTa                                                        <  2:472986/139‑1 (MQ=255)                                                                        cggATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTa                                                        <  2:117550/139‑1 (MQ=255)                                                                            tGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                               <  1:1661819/112‑1 (MQ=255)                                                                            tGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                               >  2:1661819/1‑112 (MQ=255)                                                                             gagaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                   <  1:344831/139‑1 (MQ=255)                                                                                 ttGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTtgt                                               >  1:1691734/1‑139 (MQ=255)                                                                                    cgcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGcc                                            <  1:1369776/139‑1 (MQ=255)                                                                                            ttGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTAACGCGTCTTATCAt                                                             >  2:1365642/1‑114 (MQ=255)                                                                                            ttGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt                                                             <  1:1365642/114‑1 (MQ=255)                                                                                               cTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGc                                 <  2:1691734/139‑1 (MQ=255)                                                                                                         cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                               >  2:839288/1‑67 (MQ=255)                                                                                                         cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                               <  1:839288/67‑1 (MQ=255)                                                                                                         cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCg                                                                                  <  2:769882/80‑1 (MQ=255)                                                                                                         cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCg                                                                                  >  1:769882/1‑80 (MQ=255)                                                                                                            ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTgg                                                                                                    >  2:663154/1‑59 (MQ=255)                                                                                                            ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTgg                                                                                                    <  1:663154/59‑1 (MQ=255)                                                                                                            ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGACGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGtt                    >  2:1261094/1‑139 (MQ=255)                                                                                                             taCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGc                                 <  1:89452/125‑1 (MQ=255)                                                                                                             taCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGc                                 >  2:89452/1‑125 (MQ=255)                                                                                                                  tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACaa                                                                                             >  2:192026/1‑60 (MQ=255)                                                                                                                  tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACaa                                                                                             <  1:192026/60‑1 (MQ=255)                                                                                                                  tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgcc              >  2:294807/1‑139 (MQ=255)                                                                                                                   gttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt                                                                                                      >  2:170851/1‑50 (MQ=255)                                                                                                                   gttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt                                                                                                      <  1:170851/50‑1 (MQ=255)                                                                                                                    tttAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                                                                                            <  2:1743405/59‑1 (MQ=255)                                                                                                                    tttAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                                                                                            >  1:1743405/1‑59 (MQ=255)                                                                                                                    tttAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgccgc            >  2:1442064/1‑139 (MQ=255)                                                                                                                            gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                   >  2:181810/1‑92 (MQ=255)                                                                                                                            gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                   <  1:181810/92‑1 (MQ=255)                                                                                                                             ccGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAg   <  2:1034765/139‑1 (MQ=255)                                                                                                                              cGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGt  >  2:382564/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                       GGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGT  >  NC_000913/4614131‑4614394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑