Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,059	C→T	*186* (TAG→TAA)	yaiS ←	putative PIG‑L family deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,059	0	C	T	100.0%	188.1 / NA	58	*186* (TAG→TAA)	yaiS	putative PIG‑L family deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

CCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAG  >  NC_000913/383924‑384194                                                                                                                                        |                                                                                                                                        ccAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAg                                                                                                                                      >  1:1086325/1‑139 (MQ=255)                  ttGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTaa                                                                                                                     <  1:929482/139‑1 (MQ=255)                      tCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  2:1714829/139‑1 (MQ=255)                      tCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  2:1425794/139‑1 (MQ=255)                      tCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  1:671132/139‑1 (MQ=255)                      tCAAAGAATCATTTTATGAATGACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  2:1086325/139‑1 (MQ=255)                        aaaGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgcagc                                                                                                               <  1:614586/139‑1 (MQ=255)                                  tttATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     >  2:800946/1‑139 (MQ=255)                                  tttATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     >  1:192917/1‑139 (MQ=255)                                    tATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGt                                                                                                                             <  1:1050491/113‑1 (MQ=255)                                    tATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGt                                                                                                                             >  2:1050491/1‑113 (MQ=255)                                                    ttCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGttt                                                                                                         >  1:1615669/1‑117 (MQ=255)                                                    ttCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGttt                                                                                                         <  2:1615669/117‑1 (MQ=255)                                                    ttCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAtt                                                                                           <  1:1739973/131‑1 (MQ=255)                                                    ttCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAtt                                                                                           >  2:1739973/1‑131 (MQ=255)                                                     tCACGCAGATCCCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATAGAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTaa                                                                                  >  1:1066160/1‑139 (MQ=255)                                                        cccAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                               <  2:1066160/139‑1 (MQ=255)                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGt                                                                                                           >  1:1475579/1‑107 (MQ=255)                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGt                                                                                                           <  2:1475579/107‑1 (MQ=255)                                                             aTCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCa                                                                          <  2:143674/139‑1 (MQ=255)                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACtgtg                                                                                                  >  1:1107792/1‑105 (MQ=255)                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACtgtg                                                                                                  <  2:1107792/105‑1 (MQ=255)                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     <  2:1110317/102‑1 (MQ=255)                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     >  1:1110317/1‑102 (MQ=255)                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAg                                                                >  2:1064418/1‑139 (MQ=255)                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 >  1:429197/1‑85 (MQ=255)                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  2:429197/85‑1 (MQ=255)                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                               >  1:680263/1‑119 (MQ=255)                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                               <  2:680263/119‑1 (MQ=255)                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATa                                                           >  2:826877/1‑139 (MQ=255)                                                                            ttgtCCGCGAAATTGTGAAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATa                                                           >  1:1049330/1‑139 (MQ=255)                                                                                 cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGt                                                                                                                             >  2:1480829/1‑68 (MQ=255)                                                                                 cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGt                                                                                                                             <  1:1480829/68‑1 (MQ=255)                                                                                 cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGa                                                      >  1:1544278/1‑139 (MQ=255)                                                                                   cgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 >  2:361828/1‑78 (MQ=255)                                                                                   cgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  1:361828/78‑1 (MQ=255)                                                                                                     cGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGttt                                  <  1:844740/139‑1 (MQ=255)                                                                                                           cGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGc                            >  1:187443/1‑139 (MQ=255)                                                                                                                    cGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAatat                                                                                            >  2:239803/1‑66 (MQ=255)                                                                                                                    cGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAatat                                                                                            <  1:239803/66‑1 (MQ=255)                                                                                                                        tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAACAAGTTTGACTGTGAAATATTCTTCTTTaa                                                                                  <  2:393848/72‑1 (MQ=255)                                                                                                                        tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAACAAGTTTGACTGTGAAATATTCTTCTTTaa                                                                                  >  1:393848/1‑72 (MQ=255)                                                                                                                           aaTCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCa                                                             >  1:1567125/1‑90 (MQ=255)                                                                                                                           aaTCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCa                                                             <  2:1567125/90‑1 (MQ=255)                                                                                                                            aTCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                >  2:165535/1‑102 (MQ=255)                                                                                                                            aTCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                <  1:165535/102‑1 (MQ=255)                                                                                                                             tCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAg          >  2:122153/1‑139 (MQ=255)                                                                                                                              cgcgTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaaa                                                         >  1:572672/1‑91 (MQ=255)                                                                                                                              cgcgTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaaa                                                         <  2:572672/91‑1 (MQ=255)                                                                                                                               gcgTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGtt        <  2:187443/139‑1 (MQ=255)                                                                                                                                cgtcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     <  2:1638749/45‑1 (MQ=255)                                                                                                                                cgtcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     >  1:1638749/1‑45 (MQ=255)                                                                                                                                cgtcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAatat                                                                                            >  1:41911/1‑54 (MQ=255)                                                                                                                                cgtcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAatat                                                                                            <  2:41911/54‑1 (MQ=255)                                                                                                                                  tcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACg                                                                                >  2:1629854/1‑64 (MQ=255)                                                                                                                                  tcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACg                                                                                <  1:1629854/64‑1 (MQ=255)                                                                                                                                  tcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCgg     <  1:772579/139‑1 (MQ=255)                                                                                                                                     ttCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCggcag  >  2:55475/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        CCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAG  >  NC_000913/383924‑384194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑