Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	427,675	A→G	Y10C (TAC→TGC)	secD →	SecYEG protein translocase auxillary subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	427,675	0	A	G	100.0%	245.3 / NA	68	Y10C (TAC→TGC)	secD	SecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/34);  total (34/34)

TGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGT  >  NC_000913/427544‑427804                                                                                                                                    |                                                                                                                                  tGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCg                                                                                                                                 >  2:141882/1‑134 (MQ=255) tGAAACCCCTGAGCTAGTTATTAAGCGTGACGTCGTAGCTGCCTTCCTGCCGAAAGGCACCATGAATGAGCTGTAAGTAAAATTTTTCCCTAAGGGAATTGCCGTATTAAACCCTTATCCATTGGGAAAGTGCGTCATg                                                                                                                            <  1:901294/139‑1 (MQ=255)    caccacTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTg                                                                                                                         >  1:1399256/1‑139 (MQ=255)         cTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCgt                                                                                                                    >  2:61560/1‑139 (MQ=255)              gtagtTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGAt                                                                                                                       <  1:688080/131‑1 (MQ=255)              gtagtTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGAt                                                                                                                       >  2:688080/1‑131 (MQ=255)                 gtTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTg                                                                                                            >  2:1116442/1‑139 (MQ=255)                 gtTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTg                                                                                                            >  2:588744/1‑139 (MQ=255)                    attaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCa                                                                                                         >  2:131466/1‑139 (MQ=255)                    attaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCa                                                                                                         >  1:913181/1‑139 (MQ=255)                      taAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATc                                                                                                       >  2:54665/1‑139 (MQ=255)                         aCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGt                                                                                                    >  1:1825023/1‑139 (MQ=255)                            tGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTctg                                                                                                 >  1:1715945/1‑139 (MQ=255)                                  cGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTAt                                                                                           >  2:247815/1‑139 (MQ=255)                                   gTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATg                                                                                          <  2:1825023/139‑1 (MQ=255)                                    tAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATgc                                                                                         <  1:247815/139‑1 (MQ=255)                                      gCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATgcgc                                                                                       <  2:1715945/139‑1 (MQ=255)                                       cTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATc                                                                                                                      <  2:510529/107‑1 (MQ=255)                                       cTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATc                                                                                                                      >  1:510529/1‑107 (MQ=255)                                         gCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTc                                                                                    <  2:165620/139‑1 (MQ=255)                                         gCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTc                                                                                    >  2:1273167/1‑139 (MQ=255)                                              ccTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAAc                                                                               <  2:1399256/139‑1 (MQ=255)                                                  ccGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCg                                                                                                                                 >  1:328802/1‑85 (MQ=255)                                                       aGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTctg                                                                                                 >  1:282028/1‑112 (MQ=255)                                                       aGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTctg                                                                                                 <  2:282028/112‑1 (MQ=255)                                                            ccATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGa                                                                 <  1:1273167/139‑1 (MQ=255)                                                              aTGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATc                                                               >  2:1288139/1‑139 (MQ=255)                                                                 aaGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCgg                                                            <  1:1145995/139‑1 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCg                                                                                                                                 <  1:513226/64‑1 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCg                                                                                                                     <  1:851712/76‑1 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCg                                                                                                                     >  2:851712/1‑76 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTa                                                                                            >  1:489411/1‑101 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTa                                                                                            <  2:489411/101‑1 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCgg                                                            <  1:1273390/133‑1 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCgg                                                            >  2:1273390/1‑133 (MQ=255)                                                                       cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTc                                                      >  2:1165919/1‑139 (MQ=255)                                                                        tGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCa                                                     <  1:131466/139‑1 (MQ=255)                                                                                       tCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTgcgcg                                      <  1:705628/139‑1 (MQ=255)                                                                                          cTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGt                                                                      >  1:775982/1‑104 (MQ=255)                                                                                          cTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGt                                                                      <  2:775982/104‑1 (MQ=255)                                                                                             aGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGt                                <  1:588744/139‑1 (MQ=255)                                                                                                   ttGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCa                                                                                                         <  2:968130/60‑1 (MQ=255)                                                                                                   ttGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCa                                                                                                         >  1:968130/1‑60 (MQ=255)                                                                                                   ttGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTcgccgc                          >  2:1512567/1‑139 (MQ=255)                                                                                                   ttGCCATGTTAAACCGTTATCCTTTGTGGAAGTGCGt                                                                                                                                >  2:905444/1‑37 (MQ=255)                                                                                                   ttGCCATGTTAAACCGTTATCCTTTGTGGAAGTGCGt                                                                                                                                <  1:905444/37‑1 (MQ=255)                                                                                                     gCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGccacca                        <  2:1007315/139‑1 (MQ=255)                                                                                                       cGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGttt                                                                         <  2:153357/88‑1 (MQ=255)                                                                                                       cGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGttt                                                                         >  1:153357/1‑88 (MQ=255)                                                                                                          gtTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCAC‑GGTGCGCGCGGAGTCGCCGCCAGTGAGc                  <  1:1116442/139‑1 (MQ=255)                                                                                                            tAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCa                 <  1:1165919/139‑1 (MQ=255)                                                                                                                ccGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCa                 <  2:410412/135‑1 (MQ=255)                                                                                                                ccGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCa                 >  1:410412/1‑135 (MQ=255)                                                                                                                ccGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACg             <  1:54665/139‑1 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGt                                                                                                    >  2:351978/1‑49 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGt                                                                                                    <  1:351978/49‑1 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTccc                                                                                  >  1:793888/1‑67 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTccc                                                                                  <  2:793888/67‑1 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCa                                                     <  1:1700898/96‑1 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCa                                                     >  2:1700898/1‑96 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGa                                  >  1:467794/1‑115 (MQ=255)                                                                                                                   ttATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGa                                  <  2:467794/115‑1 (MQ=255)                                                                                                                      tCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATc       <  1:1512567/139‑1 (MQ=255)                                                                                                                        cTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCa     <  2:1267954/139‑1 (MQ=255)                                                                                                                           tgtgGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTgcgcg                                      <  1:1265887/103‑1 (MQ=255)                                                                                                                           tgtgGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTgcgcg                                      >  2:1265887/1‑103 (MQ=255)                                                                                                                           tgtgGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGt  >  1:1316852/1‑139 (MQ=255)                                                                                                                           tgtgGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGt  >  2:1379830/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                  TGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGT  >  NC_000913/427544‑427804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑