Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,764	C→T	A283T (GCT→ACT)	cyoA ←	cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,764	0	C	T	100.0%	150.6 / NA	47	A283T (GCT→ACT)	cyoA	cytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/20);  total (27/20)

CGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGC  >  NC_000913/450635‑450894                                                                                                                                  |                                                                                                                                    cGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  1:607205/139‑1 (MQ=255) cGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  1:568582/139‑1 (MQ=255)          ttATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTAc                                                                                                                   >  1:1744725/1‑139 (MQ=255)                  ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATa                                                                                                                                 <  1:1168781/117‑1 (MQ=255)                  ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATa                                                                                                                                 >  2:1168781/1‑117 (MQ=255)                  ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                           >  2:548443/1‑139 (MQ=255)                  ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                           >  1:1548469/1‑139 (MQ=255)                  ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                           >  1:1834407/1‑139 (MQ=255)                            tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc                                                                                                                              <  2:44562/110‑1 (MQ=255)                            tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc                                                                                                                              >  1:44562/1‑110 (MQ=255)                                    gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            >  2:67314/1‑104 (MQ=255)                                    gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  1:67314/104‑1 (MQ=255)                                     cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCa                                                                                                                                   >  2:203126/1‑96 (MQ=255)                                       gATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTgg                                                                                      >  1:827221/1‑139 (MQ=255)                                              cgTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTc                                                                                                    <  2:881133/118‑1 (MQ=255)                                              cgTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTc                                                                                                    >  1:881133/1‑118 (MQ=255)                                                           cATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTg                                                                  >  1:1453668/1‑139 (MQ=255)                                                             tGCCTTCCATACCTTTGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg                                                                                          <  1:1099253/113‑1 (MQ=255)                                                             tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            >  1:1739662/1‑79 (MQ=255)                                                             tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  2:1739662/79‑1 (MQ=255)                                                             tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTcgcg                                                                                          >  2:1099253/1‑113 (MQ=255)                                                                   ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCt                                                         >  1:747909/1‑139 (MQ=255)                                                                    cATACCTTCGTGTGCACTGTGCTCACCTTCTGGCTGGATCATGTCCATGCTCTTACCGTGAGTCATAAACTTGCTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTa                                                        >  1:1226952/1‑139 (MQ=255)                                                                              tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCa                                              >  1:1009042/1‑139 (MQ=255)                                                                                   gcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGtttt                                         <  1:548443/139‑1 (MQ=255)                                                                                       tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                       >  1:1049089/1‑90 (MQ=255)                                                                                       tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                       <  2:1049089/90‑1 (MQ=255)                                                                                             ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  2:59694/139‑1 (MQ=255)                                                                                             ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  2:1009042/139‑1 (MQ=255)                                                                                              cTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc                              <  2:1744725/139‑1 (MQ=255)                                                                                                 ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                     >  1:1614760/1‑98 (MQ=255)                                                                                                 ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                     <  2:1614760/98‑1 (MQ=255)                                                                                                 ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTTCGCTAGGCGCGGCCAGTTTTTc                                       >  1:1683665/1‑128 (MQ=255)                                                                                                 ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTTCGCTAGGCGCGGCCAGTTTTTc                                       <  2:1683665/128‑1 (MQ=255)                                                                                                 ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                           >  2:1514979/1‑139 (MQ=255)                                                                                                 ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                           >  1:207007/1‑139 (MQ=255)                                                                                                      tggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAg                      >  1:212098/1‑139 (MQ=255)                                                                                                              gTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  2:460099/122‑1 (MQ=255)                                                                                                              gTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               >  1:460099/1‑122 (MQ=255)                                                                                                                    gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCt                                                                                                   <  2:1740628/49‑1 (MQ=255)                                                                                                                    gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCt                                                                                                   >  1:1740628/1‑49 (MQ=255)                                                                                                                    gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGc                                 >  2:1799713/1‑114 (MQ=255)                                                                                                                    gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGc                                 <  1:1799713/114‑1 (MQ=255)                                                                                                                     ctctTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg       <  2:1834407/139‑1 (MQ=255)                                                                                                                         tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                     <  2:1755611/42‑1 (MQ=255)                                                                                                                         tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                     >  1:1755611/1‑42 (MQ=255)                                                                                                                          aCCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc  >  1:1821900/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                    CGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGC  >  NC_000913/450635‑450894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑