Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	469,429	A→G	K187E (AAG→GAG)	mdlA →	putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,429	0	A	G	100.0%	186.1 / NA	53	K187E (AAG→GAG)	mdlA	putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

ACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGC  >  NC_000913/469293‑469550                                                                                                                                         |                                                                                                                          aCTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                         >  2:560361/1‑139 (MQ=255)          gggCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAg                                                                                                                >  1:195878/1‑139 (MQ=255)           ggCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAgg                                                                                                               >  1:949632/1‑139 (MQ=255)                     tgtTGATTATTATGTCTAAGCAACTTAGCTGGGAGTTGAACTTATTTTCCATGTTGCCGATGCCAGCGATGGCGATCATGATTAAGCTCAGCCGCGATGATTTGCTTGAACGCTTTGAGCTGGCACAGGCGGCGTCTTc                                                                                                     >  1:29996/1‑139 (MQ=255)                     tgtTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                     <  2:195878/139‑1 (MQ=255)                               gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                         >  2:404695/1‑109 (MQ=255)                               gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                         <  1:404695/109‑1 (MQ=255)                               gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcggc                                                                                                           <  2:656325/123‑1 (MQ=255)                               gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcggc                                                                                                           >  1:656325/1‑123 (MQ=255)                               gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAAt                                                                                           >  2:1517189/1‑139 (MQ=255)                                 tgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGa                                                                                         <  2:1590709/139‑1 (MQ=255)                                    cTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                         <  1:840420/104‑1 (MQ=255)                                    cTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCg                                                                                      <  2:1517137/139‑1 (MQ=255)                                     tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAgg                                                                                                               >  2:542422/1‑113 (MQ=255)                                     tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAgg                                                                                                               <  1:542422/113‑1 (MQ=255)                                       cGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCAc                                                                                   <  1:560361/139‑1 (MQ=255)                                            aTTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAgg                                                                              <  1:1517189/139‑1 (MQ=255)                                               aGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGaaa                                                                           >  2:1411766/1‑139 (MQ=255)                                                    gCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCt                                                                       <  1:830979/138‑1 (MQ=255)                                                    gCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCt                                                                       >  2:830979/1‑138 (MQ=255)                                                      aGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                    <  2:609252/139‑1 (MQ=255)                                                                       cTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCa                                                   >  2:1115224/1‑139 (MQ=255)                                                                           tGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGc                                               >  1:1019388/1‑139 (MQ=255)                                                                                  gCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGt                                        >  2:854491/1‑139 (MQ=255)                                                                                    cAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCt                                      <  1:1115224/139‑1 (MQ=255)                                                                                       tGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                     >  1:539171/1‑73 (MQ=255)                                                                                       tGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                     <  2:539171/73‑1 (MQ=255)                                                                                        gATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGaa                                  <  1:854491/139‑1 (MQ=255)                                                                                            gCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                          <  1:221703/63‑1 (MQ=255)                                                                                            gCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                          >  2:221703/1‑63 (MQ=255)                                                                                            gCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATc                              <  2:949632/139‑1 (MQ=255)                                                                                                tCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCa                          >  1:1256087/1‑139 (MQ=255)                                                                                                         aGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAg                                                                                                  >  2:1460934/1‑58 (MQ=255)                                                                                                         aGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAg                                                                                                  <  1:1460934/58‑1 (MQ=255)                                                                                                         aGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGtt                 >  1:960595/1‑139 (MQ=255)                                                                                                          gcgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTa                >  1:1317942/1‑139 (MQ=255)                                                                                                           cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCa                                                                                                   <  2:1606470/55‑1 (MQ=255)                                                                                                           cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCa                                                                                                   >  1:1606470/1‑55 (MQ=255)                                                                                                           cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGaa                                                                            >  1:1056559/1‑78 (MQ=255)                                                                                                           cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGaa                                                                            <  2:1056559/78‑1 (MQ=255)                                                                                                           cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGcc                                                                        <  1:1176806/82‑1 (MQ=255)                                                                                                           cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGcc                                                                        >  2:1176806/1‑82 (MQ=255)                                                                                                              aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAgg                                                                              >  2:675179/1‑73 (MQ=255)                                                                                                              aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAgg                                                                              <  1:675179/73‑1 (MQ=255)                                                                                                               aCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGc                                                          <  2:1501342/92‑1 (MQ=255)                                                                                                               aCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGc                                                          >  1:1501342/1‑92 (MQ=255)                                                                                                                     aTGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGt                        >  1:1716672/1‑120 (MQ=255)                                                                                                                     aTGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGt                        <  2:1716672/120‑1 (MQ=255)                                                                                                                        cTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGc  >  2:1258368/1‑139 (MQ=255)                                                                                                                                  aCGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCt                                             >  1:896799/1‑86 (MQ=255)                                                                                                                                  aCGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCt                                             <  2:896799/86‑1 (MQ=255)                                                                                                                                   cGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATc                                                    <  1:830331/78‑1 (MQ=255)                                                                                                                                   cGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATc                                                    >  2:830331/1‑78 (MQ=255)                                                                                                                                         |                                                                                                                          ACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGC  >  NC_000913/469293‑469550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑