Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	470,643	G→A	*591* (TAG→TAA)  S3N (AGT→AAT)	mdlA → mdlB →	putative multidrug ABC transporter ATPase putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	470,643	0	G	A	100.0%	149.1 / NA	47	*591* (TAG→TAA)  S3N (AGT→AAT)	mdlA mdlB	putative multidrug ABC transporter ATPase putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

GCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCG  >  NC_000913/470515‑470764                                                                                                                                 |                                                                                                                          gCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCaa                                                                                                                 <  2:301825/139‑1 (MQ=255)      gCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACtgtg                                                                                                            >  1:1188869/1‑139 (MQ=255)       cGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTgg                                                                                                           >  2:31960/1‑139 (MQ=255)         tGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGcc                                                                                                         <  2:1188869/139‑1 (MQ=255)          ggCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCg                                                                                                        <  2:1828582/139‑1 (MQ=255)                cATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACtctc                                                                                                  <  2:140024/139‑1 (MQ=255)                cATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACtctc                                                                                                  <  1:679916/139‑1 (MQ=255)                     tgtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgcg                                                                                             >  2:634450/1‑139 (MQ=255)                     tgtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAgcg                                                                                             <  1:634450/139‑1 (MQ=255)                       tgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                           <  2:1384924/139‑1 (MQ=255)                         cTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTg                                                                                         >  1:874707/1‑139 (MQ=255)                            gCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGTCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTa                                                                                      >  2:23974/1‑139 (MQ=255)                               caacaaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCg                                                                                   <  2:874707/139‑1 (MQ=255)                                          cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGTCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                        <  1:23974/139‑1 (MQ=255)                                          cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                        <  1:1260139/139‑1 (MQ=255)                                          cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                        <  1:31960/139‑1 (MQ=255)                                                       atGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          <  2:807122/92‑1 (MQ=255)                                                       atGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          >  1:807122/1‑92 (MQ=255)                                                        tGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGc                                                          >  2:1345546/1‑139 (MQ=255)                                                        tGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGc                                                          >  2:1370313/1‑139 (MQ=255)                                                             cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          <  1:1611017/86‑1 (MQ=255)                                                             cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          >  2:1611017/1‑86 (MQ=255)                                                                tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                           <  2:313023/98‑1 (MQ=255)                                                                tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                           >  1:313023/1‑98 (MQ=255)                                                                tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTa                                                                <  1:1487351/125‑1 (MQ=255)                                                                tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTa                                                                >  2:1487351/1‑125 (MQ=255)                                                                tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGAtt                                                  <  2:546831/139‑1 (MQ=255)                                                                  tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          >  2:704491/1‑81 (MQ=255)                                                                  tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          <  1:704491/81‑1 (MQ=255)                                                                  tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                >  1:756886/1‑139 (MQ=255)                                                                  tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                >  1:670679/1‑139 (MQ=255)                                                                  tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                >  2:208183/1‑139 (MQ=255)                                                                    aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                              <  1:1370313/139‑1 (MQ=255)                                                                    aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                              >  2:615066/1‑139 (MQ=255)                                                                         cTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                         <  1:325772/139‑1 (MQ=255)                                                                         cTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                         >  2:576775/1‑139 (MQ=255)                                                                         cTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                         <  2:670679/139‑1 (MQ=255)                                                                            gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          >  2:1648620/1‑71 (MQ=255)                                                                            gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGc                                                                                                          <  1:1648620/71‑1 (MQ=255)                                                                            gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtgatg                                      >  2:1316053/1‑139 (MQ=255)                                                                                   cgcTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGt                                                                             <  2:1671327/93‑1 (MQ=255)                                                                                   cgcTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGt                                                                             >  1:1671327/1‑93 (MQ=255)                                                                                             cgCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTgcggcggc                     >  1:91943/1‑139 (MQ=255)                                                                                                    gAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGaa              <  1:1345546/139‑1 (MQ=255)                                                                                                                gaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCg  <  1:1316053/139‑1 (MQ=255)                                                                                                                   gCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                        >  1:1099274/1‑66 (MQ=255)                                                                                                                   gCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                        <  2:1099274/66‑1 (MQ=255)                                                                                                                                 |                                                                                                                          GCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCG  >  NC_000913/470515‑470764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑