Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,869,022 (T)6→5 coding (68/1284 nt) yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,869,0170T.100.0% 181.0 / NA 44coding (63/1284 nt)yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/22);  total (22/22)

AGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAT  >  NC_000913/1868884‑1869142
                                                                                                                                     |                                                                                                                             
aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                          <  1:1193686/139‑2 (MQ=255)
  cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGACCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                        >  2:1058379/1‑136 (MQ=255)
       cGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                          >  1:1012729/1‑131 (MQ=255)
       cGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                          <  2:1012729/132‑2 (MQ=255)
                         cAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAAc                                                                                                <  1:1248931/139‑1 (MQ=255)
                              caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                           <  2:77994/139‑1 (MQ=255)
                                      gCATACAGCTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                   <  1:831027/139‑1 (MQ=255)
                                                 gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                    <  1:1270674/127‑1 (MQ=255)
                                                 gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                    >  2:1270674/1‑127 (MQ=255)
                                                        tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                    <  2:656966/120‑1 (MQ=255)
                                                        tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                    >  1:656966/1‑120 (MQ=255)
                                                           taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                            >  1:902511/1‑109 (MQ=255)
                                                           taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                            <  2:902511/109‑1 (MQ=255)
                                                                  ggCATATGACCTGGTTTATTTACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                       >  2:1071018/1‑139 (MQ=255)
                                                                  ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 >  1:1174287/1‑113 (MQ=255)
                                                                  ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 <  2:1174287/113‑1 (MQ=255)
                                                                  ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                       >  1:114397/1‑139 (MQ=255)
                                                                  ggCATATGACCTGGTTTATTAACCGGCGTCTGAACGGAAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGTCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                       <  1:435812/139‑1 (MQ=255)
                                                                     atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTGATAAGCGTTCGGTGACTGACGTc                                                    >  2:998655/1‑139 (MQ=255)
                                                                            cTGGTTTATTGACCGGCGTCTGAACGGCAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                        >  1:195232/1‑61 (MQ=255)
                                                                            cTGGTTTATTGACCGGCGTCTGAACGGCAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                        <  2:195232/64‑4 (MQ=255)
                                                                             tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcgg                                            >  2:1241427/1‑139 (MQ=255)
                                                                             tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcgg                                            <  1:1058379/139‑1 (MQ=255)
                                                                              ggTTTATTTACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                           <  1:1071018/139‑1 (MQ=255)
                                                                              ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 >  2:1298338/1‑101 (MQ=255)
                                                                              ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 <  1:1298338/101‑1 (MQ=255)
                                                                                 ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                          <  2:219202/58‑2 (MQ=255)
                                                                                 ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                          >  1:219202/1‑57 (MQ=255)
                                                                                    ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                        >  1:835946/1‑54 (MQ=255)
                                                                                    ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGcttttacg                                                                                                                        <  2:835946/57‑4 (MQ=255)
                                                                                    ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                      <  1:175956/106‑1 (MQ=255)
                                                                                    ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                      >  2:175956/1‑106 (MQ=255)
                                                                                      gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                    <  1:1347184/90‑1 (MQ=255)
                                                                                      gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                    >  2:1347184/1‑90 (MQ=255)
                                                                                         cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 >  1:716783/1‑90 (MQ=255)
                                                                                         cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 <  2:716783/90‑1 (MQ=255)
                                                                                         cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                >  2:259217/1‑139 (MQ=255)
                                                                                         cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                >  1:29441/1‑139 (MQ=255)
                                                                                            cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                             <  2:114397/139‑1 (MQ=255)
                                                                                            cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTACCCAt                             >  2:1065631/1‑139 (MQ=255)
                                                                                              tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTa                                                                                                   >  1:1159681/1‑67 (MQ=255)
                                                                                              tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTa                                                                                                   <  2:1159681/67‑1 (MQ=255)
                                                                                              tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                           >  2:707070/1‑139 (MQ=255)
                                                                                              tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                           >  1:1285811/1‑139 (MQ=255)
                                                                                                            aCAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                             <  2:500117/123‑1 (MQ=255)
                                                                                                            aCAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                             >  1:500117/1‑123 (MQ=255)
                                                                                                                     tGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg    <  1:687642/139‑1 (MQ=255)
                                                                                                                      ggTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                          >  2:589121/1‑68 (MQ=255)
                                                                                                                      ggTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                          <  1:589121/68‑1 (MQ=255)
                                                                                                                       gTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt  <  1:707070/139‑1 (MQ=255)
                                                                                                                       gTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTACCCATTCCCACGGAAGATATTAGCGAACCGAt  <  1:1065631/139‑1 (MQ=255)
                                                                                                                          aaTCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                          <  1:971494/96‑1 (MQ=255)
                                                                                                                          aaTCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                          >  2:971494/1‑96 (MQ=255)
                                                                                                                            tCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 <  1:726812/55‑1 (MQ=255)
                                                                                                                            tCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                 >  2:726812/1‑55 (MQ=255)
                                                                                                                                     |                                                                                                                             
AGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAT  >  NC_000913/1868884‑1869142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: