Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,607 Δ1 bp coding (1145/1158 nt) cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,6070A.96.5% 110.7 / ‑2.9 29coding (1145/1158 nt)cdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (19/9);  minor base G (1/0);  total (20/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTT  >  NC_000913/183492‑183740
                                                                                                                   |                                                                                                                                     
ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTa                                                                                                               >  2:636590/1‑139 (MQ=255)
           tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                      <  2:177943/121‑1 (MQ=255)
           tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                      >  1:177943/1‑121 (MQ=255)
           tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCATGATTTGGGCAATTTTGATGACAGGTTGCGGCTGTATGTGGCGTTACAACGGGTGAAGAGCGGTAAGTTATGCGTTAAGGATGAggcg                                                                                                    >  1:662557/1‑139 (MQ=255)
                aTACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCt                                                                                               <  1:636590/139‑1 (MQ=255)
                      tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                         >  2:629416/1‑139 (MQ=255)
                           tATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                    >  2:116250/1‑139 (MQ=255)
                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                  >  2:782269/1‑139 (MQ=255)
                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                  >  2:256911/1‑139 (MQ=255)
                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                  >  2:132294/1‑139 (MQ=255)
                              cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                 >  1:72100/1‑123 (MQ=255)
                              cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                 <  2:72100/123‑1 (MQ=255)
                                  ttAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGcc                                                                             >  1:169388/1‑139 (MQ=255)
                                    aaTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                           <  1:1230435/139‑1 (MQ=255)
                                           tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                    >  2:673256/1‑139 (MQ=255)
                                                gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                               >  2:757074/1‑139 (MQ=255)
                                                   cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                            >  1:395940/1‑139 (MQ=255)
                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                         >  2:636342/1‑139 (MQ=255)
                                                                 tttGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAcc                                              <  1:629416/139‑1 (MQ=255)
                                                                     ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                          >  2:1257806/1‑139 (MQ=255)
                                                                     ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                          >  2:1187827/1‑139 (MQ=255)
                                                                               atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGatt                                >  2:1369259/1‑139 (MQ=255)
                                                                                    aGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTa                           >  2:1350997/1‑139 (MQ=255)
                                                                                      gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                         >  2:17318/1‑107 (MQ=255)
                                                                                      gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                         <  1:17318/107‑1 (MQ=255)
                                                                                      gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcg                         >  2:744690/1‑139 (MQ=255)
                                                                                             ctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGt                  <  1:132294/139‑1 (MQ=255)
                                                                                                      gCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGa         <  1:1257806/139‑1 (MQ=255)
                                                                                                             aaCTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCtt  <  2:1214528/139‑1 (MQ=255)
                                                                                                                   |                                                                                                                                     
TTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTT  >  NC_000913/183492‑183740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: