Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,200,454	(A)8→7	coding (176/3633 nt)	yehI →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,200,447	0	A	.	100.0%	99.3 / NA	25	coding (169/3633 nt)	yehI	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/13);  total (12/13)

AACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCG  >  NC_000913/2200316‑2200566                                                                                                                                    |                                                                                                                        aaCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaac                                                                                                                  <  2:592260/139‑2 (MQ=255)  aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                 <  2:202947/139‑3 (MQ=255)      gAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGt                                                                                                            >  2:415431/1‑139 (MQ=255)          tGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTgg                                                                                                        <  1:415431/139‑1 (MQ=255)                            aaaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCa                                                                                      >  1:979630/1‑139 (MQ=255)                                 ccgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGa                                                                                 <  2:979630/139‑1 (MQ=255)                                         tCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGt                                                                         >  2:1107561/1‑139 (MQ=255)                                                   tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                               >  1:907240/1‑139 (MQ=255)                                                          gCGAACCGGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                        <  1:1025932/139‑1 (MQ=255)                                                          gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                        >  2:225934/1‑139 (MQ=255)                                                                cAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAAc                                                  <  2:751658/139‑1 (MQ=255)                                                                    gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                              <  1:717040/139‑1 (MQ=255)                                                                        gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAAGTTGATCgg                                          >  2:811413/1‑139 (MQ=255)                                                                         ttATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGc                                         <  2:907240/139‑1 (MQ=255)                                                                                 ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                 >  1:1000783/1‑139 (MQ=255)                                                                                        tcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCa                          >  1:663183/1‑139 (MQ=255)                                                                                           tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                       <  2:663183/139‑1 (MQ=255)                                                                                               gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCAt                                                                  <  1:420083/92‑1 (MQ=255)                                                                                               gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCAt                                                                  >  2:420083/1‑92 (MQ=255)                                                                                                aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACg                                                              <  2:995851/95‑1 (MQ=255)                                                                                                aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACg                                                              >  1:995851/1‑95 (MQ=255)                                                                                                  aTTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                            >  2:840635/1‑127 (MQ=255)                                                                                                  aTTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                            <  1:840635/127‑1 (MQ=255)                                                                                                          atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAAc                                                  >  2:1265879/1‑97 (MQ=255)                                                                                                          atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAAc                                                  <  1:1265879/97‑1 (MQ=255)                                                                                                                tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACTAACCCTCAATCCCGGCCCGGACAATGgcggcg  <  1:811413/139‑1 (MQ=255)                                                                                                                tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg  <  2:25027/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                        AACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCG  >  NC_000913/2200316‑2200566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑