Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.96.2% 201.0 / 1.4 53intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (21/30);  minor base T (1/1);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAAT  >  NC_000913/2462743‑2463010
                                                                                                                                          |                                                                                                                                 
ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                                                   >  1:692818/1‑139 (MQ=255)
   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                                >  2:786969/1‑139 (MQ=255)
     aacatgcTGTTGGCAGCTTGCACATTCAAAGCAATGAGCGGTATGCACAGACCAACATCAAGGGAAAGGCATAGTTACAGGCGTTCTGCTCCTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                              <  1:1022365/133‑1 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                                                            <  2:1307149/139‑1 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                                                            <  1:371251/139‑1 (MQ=255)
        aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccct                                                                                                                           >  1:542307/1‑138 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctgg                                                                                                                         >  2:476531/1‑136 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                >  1:1364474/1‑139 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                              <  2:1364474/139‑1 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                          <  1:267320/139‑1 (MQ=255)
                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATa                                                                                                        <  2:1068979/139‑1 (MQ=255)
                             tgcAAATCAATAAGCGCTATCCACAGACAAACCGGAAGGGAAAGGCCTACTTTCAGCCTTTCTGCTCGTCCCTCTCCTAGAGTTGACTTTTCTGCATTTCCAGGATATTCCCCCCCCTGGCTATTGTGAGCTCATacac                                                                                                     >  1:27755/3‑139 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAATGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                                                                   <  1:786969/139‑1 (MQ=255)
                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCaaa                                                                                                <  1:563320/139‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                        <  2:1161992/139‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                        <  1:12782/139‑1 (MQ=255)
                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                >  1:572249/1‑111 (MQ=255)
                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                <  2:572249/111‑1 (MQ=255)
                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                                                    >  1:850780/1‑139 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                   <  2:259238/139‑1 (MQ=255)
                                                      gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                >  2:591894/1‑103 (MQ=255)
                                                      gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                <  1:591894/103‑1 (MQ=255)
                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                                                            >  2:254954/1‑88 (MQ=255)
                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                                                            <  1:254954/88‑1 (MQ=255)
                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                        <  1:723202/116‑1 (MQ=255)
                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                        >  2:723202/1‑116 (MQ=255)
                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAAAGGTTCTAAATAAATGAGCGttt                                                                 >  1:742300/1‑139 (MQ=255)
                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATAAACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                                 >  2:306832/1‑139 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                            <  2:542307/139‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                            <  2:822733/139‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                            <  2:1011628/139‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                            <  1:306832/139‑1 (MQ=255)
                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                          <  2:207448/139‑1 (MQ=255)
                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                <  2:850780/139‑1 (MQ=255)
                                                                                  cAGGCGTTCTACTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATATTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATTGTCTATTTCa                                                <  2:742300/139‑1 (MQ=255)
                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                        >  1:241564/1‑98 (MQ=255)
                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                        <  2:241564/98‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                              <  2:669204/139‑1 (MQ=255)
                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                          >  1:779293/1‑139 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                >  1:103533/1‑132 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                <  2:103533/132‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                         <  2:692818/139‑1 (MQ=255)
                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                           >  2:975375/1‑136 (MQ=255)
                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                           <  1:975375/136‑1 (MQ=255)
                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa                                        >  1:699575/1‑139 (MQ=255)
                                                                                             cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                     <  1:1155383/139‑1 (MQ=255)
                                                                                                       tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                           <  2:1297396/123‑1 (MQ=255)
                                                                                                       tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                           >  1:1297396/1‑123 (MQ=255)
                                                                                                         aaaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATa                                                                                                        <  1:787246/60‑1 (MQ=255)
                                                                                                         aaaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATa                                                                                                        >  2:787246/1‑60 (MQ=255)
                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                              >  1:1273078/1‑38 (MQ=255)
                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                              <  2:1273078/38‑1 (MQ=255)
                                                                                                               tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                >  2:351672/1‑46 (MQ=255)
                                                                                                               tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                                <  1:351672/46‑1 (MQ=255)
                                                                                                                 tACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaatc                 >  2:109246/1‑139 (MQ=255)
                                                                                                                       tCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATaca                                                                                                      <  2:425919/48‑1 (MQ=255)
                                                                                                                       tCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATaca                                                                                                      >  1:425919/1‑48 (MQ=255)
                                                                                                                         tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAtt                                <  1:1368861/116‑1 (MQ=255)
                                                                                                                         tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAtt                                >  2:1368861/1‑116 (MQ=255)
                                                                                                                              ttCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                   >  1:639664/1‑60 (MQ=255)
                                                                                                                              ttCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                   <  2:639664/60‑1 (MQ=255)
                                                                                                                                ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAAt  >  2:864633/1‑139 (MQ=255)
                                                                                                                                   ggATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                          <  2:499732/80‑1 (MQ=255)
                                                                                                                                   ggATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                          >  1:499732/1‑80 (MQ=255)
                                                                                                                                          |                                                                                                                                 
TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAAT  >  NC_000913/2462743‑2463010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: