Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,925	+A	coding (35/1431 nt)	yaaJ ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,922	1	.	A	100.0%	181.7 / NA	56	W13L (TGG→TTG)	yaaJ	putative transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (27/29);  total (27/29)

AACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTG  >  NC_000913/7788‑8056                                                                                                                                        |                                                                                                                                       aaCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCG‑‑GCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagg                                                                                                                                  >  2:1353107/1‑136 (MQ=255)  aCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaag                                                                                                                                   >  2:896013/1‑137 (MQ=255)    cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  1:588150/139‑5 (MQ=255)    cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  1:896013/139‑5 (MQ=255)    cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  2:587675/139‑5 (MQ=255)    cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  1:346923/139‑5 (MQ=255)    cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  2:443345/139‑5 (MQ=255)     gCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaaggac                                                                                                                                >  2:922945/1‑134 (MQ=255)     gCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaaggac                                                                                                                                <  2:148234/139‑6 (MQ=255)      ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                                <  2:291078/139‑1 (MQ=255)       cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                               <  2:471745/139‑1 (MQ=255)       cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                               <  1:1241805/139‑1 (MQ=255)       cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 >  2:511098/1‑132 (MQ=255)       cTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  1:511098/136‑5 (MQ=255)                gATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGa                                                                                                                      >  1:281573/1‑139 (MQ=255)                        cTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaag                                                                                                                                   >  1:17340/1‑115 (MQ=255)                        cTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaag                                                                                                                                   <  2:17340/117‑3 (MQ=255)                                       ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                                >  1:1297620/1‑106 (MQ=255)                                       ttGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                                <  2:1297620/106‑1 (MQ=255)                                                         tAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGt                                                                             <  2:501040/139‑1 (MQ=255)                                                            cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                                >  2:1059956/1‑85 (MQ=255)                                                            cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACg                                                                                                                                <  1:1059956/85‑1 (MQ=255)                                                                    cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAg                                                                                                                    <  1:359338/89‑1 (MQ=255)                                                                    cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAg                                                                                                                    >  2:359338/1‑89 (MQ=255)                                                                    cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACtt                                                                  >  1:87499/1‑139 (MQ=255)                                                                      caAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                                <  2:1203018/139‑1 (MQ=255)                                                                         aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc                                                                                                     >  1:1259844/1‑99 (MQ=255)                                                                         aTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc                                                                                                     <  2:1259844/99‑1 (MQ=255)                                                                              gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc                                                                                                     >  2:761333/1‑94 (MQ=255)                                                                              gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc                                                                                                     <  1:761333/94‑1 (MQ=255)                                                                              gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTaa                                                        >  1:1303075/1‑139 (MQ=255)                                                                              gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTaa                                                        >  2:470281/1‑139 (MQ=255)                                                                                           aCCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGGTACCAATTg                                           >  2:1019119/1‑139 (MQ=255)                                                                                            ccAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                                >  2:590053/1‑117 (MQ=255)                                                                                            ccAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                                <  1:590053/117‑1 (MQ=255)                                                                                            ccAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAAcaattggtaacgt                                          <  2:281573/139‑13 (MQ=255)                                                                                             caacaaCCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGtt                                         <  2:87499/139‑1 (MQ=255)                                                                                                caacCTGCGCTGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                               >  2:114439/1‑50 (MQ=255)                                                                                                caacCTGCGCTGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGc                                                                                                                               <  1:114439/50‑1 (MQ=255)                                                                                                caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTaa                                                                                                                         <  2:453853/56‑1 (MQ=255)                                                                                                caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTaa                                                                                                                         >  1:453853/1‑56 (MQ=255)                                                                                                caacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTaa                                      <  1:1353107/139‑1 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                                                                   >  2:600788/1‑71 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                                                                   >  1:149245/1‑71 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                                                                   <  2:149245/71‑1 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat                                                                                                   <  1:600788/71‑1 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCatat                                                                                             <  2:2922/77‑1 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCatat                                                                                             >  1:2922/1‑77 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgcg                                                                         <  1:54618/97‑1 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTcgcg                                                                         >  2:54618/1‑97 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 <  2:826926/40‑5 (MQ=255)                                                                                                       cgcCGAAGAGCAGGTAAATCATTACCGATCCCC‑aaaagga                                                                                                                                 >  1:826926/1‑36 (MQ=255)                                                                                                                 cAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                                <  1:368764/64‑1 (MQ=255)                                                                                                                 cAGGTAAATCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcatgca                                                                                                >  2:368764/1‑64 (MQ=255)                                                                                                                 cAGGTAAATCATTACCCATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                     <  1:470281/139‑1 (MQ=255)                                                                                                                       aaTCATTACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTAc               <  1:922945/139‑1 (MQ=255)                                                                                                                            ttACCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt          >  2:664263/1‑139 (MQ=255)                                                                                                                              aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCt                        >  2:378634/1‑123 (MQ=255)                                                                                                                              aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCt                        <  1:378634/123‑1 (MQ=255)                                                                                                                              aCCGATCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACCCTACGAGGTACTTGATaa        >  2:447448/1‑139 (MQ=255)                                                                                                                                 atcccca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                                >  2:558974/4‑79 (MQ=255)                                                                                                                                 atcccca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAACCGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc    >  2:286355/4‑139 (MQ=255)                                                                                                                                  tcccca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCctc                                                                                        <  1:1331062/52‑1 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                      <  1:749373/41‑1 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg                                                                                                      >  2:749373/1‑41 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGa                                                                     >  1:103639/1‑74 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGa                                                                     <  2:103639/74‑1 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTc                                                                <  1:558974/79‑1 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtatat                             >  1:223171/1‑114 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGtatat                             <  2:223171/114‑1 (MQ=255)                                                                                                                                  aTCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc    >  1:539925/1‑139 (MQ=255)                                                                                                                                   tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCctc                                                                                        >  2:1331062/1‑54 (MQ=255)                                                                                                                                   tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCa                                               >  2:749005/1‑95 (MQ=255)                                                                                                                                   tCCCCAAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCa                                               <  1:749005/95‑1 (MQ=255)                                                                                                                                   cccca‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTg  >  2:500450/2‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCC‑AAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTG  >  NC_000913/7788‑8056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑