| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,629 | (C)5→6 | coding (210/1071 nt) | dhaK ← | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,624 | 1 | . | C | 97.9% | 159.1 / ‑3.4 | 47 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (23/23); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCA > NC_000913/1250495‑1250756 | gTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTTAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccc‑ccgaa > 1:549875/1‑135 (MQ=255) ttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAg < 2:411796/139‑1 (MQ=255) ttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAg < 1:220286/139‑1 (MQ=255) aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCTGGACAGGCCCCC‑CGAAAGCATCCCCTGa < 2:847033/139‑1 (MQ=255) gAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATGAACTTGCATGGCGCATTCAAAGACTTTATCGGGCGTCGGTGAGGTGAAAACTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAc < 2:871382/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc < 1:562974/139‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATGAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGatat > 2:959117/1‑139 (MQ=255) gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGc > 1:882100/1‑118 (MQ=255) gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGc < 2:882100/118‑1 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc > 2:365540/1‑104 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc < 1:365540/104‑1 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCAT‑‑CCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCt < 2:59836/135‑1 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCAT‑‑CCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCt > 1:59836/1‑135 (MQ=255) acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCg < 2:637750/134‑1 (MQ=255) acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCg > 1:637750/1‑134 (MQ=255) tCGCCGCCATCAACTTGCATGGTGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 1:801388/1‑139 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATccc > 2:995097/1‑93 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATccc < 1:995097/93‑1 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:185399/137‑1 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc > 2:185399/1‑137 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 1:566652/1‑139 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGatat < 1:634709/103‑1 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGatat > 2:634709/1‑103 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 1:1220118/1‑133 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc < 2:1220118/133‑1 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa > 1:1268016/1‑94 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa < 2:1268016/94‑1 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACca > 1:846957/1‑101 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcag > 1:144681/1‑139 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCACGAAAGCATCCCCTGACCGATATAACca < 2:846957/101‑1 (MQ=255) aCTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAgg > 2:1200063/1‑139 (MQ=255) cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc > 2:885689/1‑77 (MQ=255) cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc < 1:885689/77‑1 (MQ=255) cAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCAt < 1:1016595/89‑1 (MQ=255) cAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCAt > 2:1016595/1‑89 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc < 2:1010622/62‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc > 1:1010622/1‑62 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 1:678755/1‑139 (MQ=255) gggCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc < 2:868642/139‑1 (MQ=255) ggCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCt > 2:917356/1‑139 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGCAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGAAAAAGGGGCATCAGCTc < 1:959117/139‑1 (MQ=255) cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCg > 2:168927/1‑139 (MQ=255) tgaggtgaAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGAc < 1:548415/139‑1 (MQ=255) gaggtgaAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACa < 2:144681/139‑1 (MQ=255) gCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAgg < 2:29271/86‑1 (MQ=255) gCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAgg > 1:29271/1‑86 (MQ=255) ggACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGc < 1:1200063/139‑1 (MQ=255) gACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGCTGCa > 2:902251/1‑139 (MQ=255) | GTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCA > NC_000913/1250495‑1250756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |