Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,721	(G)7→8	intergenic (‑265/+44)	ydaG ← / ← racR	Rac prophage, uncharacterized protein/Rac prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,714	1	.	G	100.0%	168.8 / NA	48	intergenic (‑258/+51)	ydaG/racR	Rac prophage, uncharacterized protein/Rac prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (25/23);  total (25/23)

GAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCAT  >  NC_000913/1419579‑1419845                                                                                                                                         |                                                                                                                                    gAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑ggg                                                                                                                                  <  2:344886/139‑1 (MQ=255) gAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTAGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑ggg                                                                                                                                  <  1:837757/139‑1 (MQ=255)      aTTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑gggggggg                                                                                                                             <  2:1059458/139‑2 (MQ=255)           ccGGCGTTCTGTGGGTCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTTATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTaaa                                                                                                                         <  2:467336/139‑1 (MQ=255)                              ggtgggtgGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGttt                                                                                                      >  2:825614/1‑139 (MQ=255)                                       tATGCTGGAACTATAGGTAATCCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGa                                                                                             <  2:803416/139‑1 (MQ=255)                                            tGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCata                                                                                        >  2:1058168/1‑139 (MQ=255)                                            tGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCata                                                                                        <  1:825614/139‑1 (MQ=255)                                                   ataGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagttt                                                                                 >  2:1199589/1‑139 (MQ=255)                                                           tGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGGATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCa                                                                         >  1:1367522/1‑139 (MQ=255)                                                           tGCCTAATTGATTGTCAATAGGCTATGCCGAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAGTCCAGGAGATTTCCGTCAGACCATTTAAGTTTATGTTCCa                                                                         >  2:914769/1‑139 (MQ=255)                                                            gCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAg                                                                        >  2:949122/1‑139 (MQ=255)                                                                       tgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGa                                                                                             >  2:932270/1‑107 (MQ=255)                                                                       tgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAAGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGa                                                                                             <  1:932270/107‑1 (MQ=255)                                                                        gTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCata                                                                                        <  2:709053/111‑1 (MQ=255)                                                                        gTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCata                                                                                        >  1:709053/1‑111 (MQ=255)                                                                         tCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGtt                                                           <  2:35536/139‑1 (MQ=255)                                                                                   aTGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCata                                                                                        <  1:1121716/100‑1 (MQ=255)                                                                                   aTGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCata                                                                                        >  2:1121716/1‑100 (MQ=255)                                                                                   aTGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTc                                                 >  2:1316548/1‑139 (MQ=255)                                                                                    tGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCa                                                                                               >  1:866893/1‑92 (MQ=255)                                                                                    tGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCa                                                                                               <  2:866893/92‑1 (MQ=255)                                                                                    tGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑gggggggg                                                                                                                             >  2:1342867/1‑60 (MQ=255)                                                                                    tGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑gggggggg                                                                                                                             <  1:1342867/61‑2 (MQ=255)                                                                                          aTGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAg                                          >  2:442532/1‑139 (MQ=255)                                                                                               ttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGAc                                                                                            <  1:353539/84‑1 (MQ=255)                                                                                               ttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGAc                                                                                            >  2:353539/1‑84 (MQ=255)                                                                                                tGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCaa                                    <  1:1058168/139‑1 (MQ=255)                                                                                                   gCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                 <  1:442532/139‑1 (MQ=255)                                                                                                   gCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                 <  1:949122/139‑1 (MQ=255)                                                                                                       aaCCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑ggg                                                                                                                                  <  1:815894/37‑1 (MQ=255)                                                                                                       aaCCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑ggg                                                                                                                                  >  2:815894/1‑37 (MQ=255)                                                                                                          cTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAg                          <  1:1316548/139‑1 (MQ=255)                                                                                                                 gTGATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTa                   >  1:1223353/1‑139 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCa                                                                                               <  2:834786/61‑1 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCa                                                                                               >  1:834786/1‑61 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAg                                                                                              >  2:574849/1‑62 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAg                                                                                              <  1:574849/62‑1 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGAc                                                                                            >  2:773821/1‑64 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGAc                                                                                            <  1:773821/64‑1 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTAcc                 <  1:1008996/139‑1 (MQ=255)                                                                                                                   gATGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTAcc                 >  2:1008996/1‑139 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAg                                                                                                            >  2:1357563/1‑47 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAg                                                                                                            <  1:1357563/47‑1 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTcc                                                                          <  2:670466/81‑1 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTcc                                                                          >  1:670466/1‑81 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                 >  2:388234/1‑122 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                 <  2:7589/122‑1 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                 <  1:388234/122‑1 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                 >  1:7589/1‑122 (MQ=255)                                                                                                                    aTGGCGACAGCAGAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTAccc                >  1:1182433/1‑139 (MQ=255)                                                                                                                           cagcagAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCa         >  2:354510/1‑139 (MQ=255)                                                                                                                            agcagAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGtt                                                                                                       >  1:1283468/1‑44 (MQ=255)                                                                                                                            agcagAAAGTGATGGGGGGGGTTAAATAACGGAATCCAGGAGtt                                                                                                       <  2:1283468/44‑1 (MQ=255)                                                                                                                                  aaGTGATGGGGGGGGTTAAATAACGGGATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCAt  <  2:1367522/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCAT  >  NC_000913/1419579‑1419845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑