Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,769,478 (G)7→8 coding (405/1113 nt) ydiK → UPF0118 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,769,4711.G100.0% 103.8 / NA 30M133R (ATG→AGG) ydiKUPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (14/16);  total (14/16)

TATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTT  >  NC_000913/1769337‑1769586
                                                                                                                                       |                                                                                                                   
tATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCAAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATgggg                                                                                                                 >  1:176420/1‑139 (MQ=255)
  tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATggggggg                                                                                                              >  2:368224/1‑139 (MQ=255)
                   ccGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCg                                                                                               <  1:444051/139‑1 (MQ=255)
                                   tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                         >  1:1162202/1‑129 (MQ=255)
                                   tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                         <  2:1162202/129‑1 (MQ=255)
                                      ccAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTg                                                                            <  2:1067717/139‑1 (MQ=255)
                                               aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                       <  1:1098189/119‑1 (MQ=255)
                                               aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                       >  2:1098189/1‑119 (MQ=255)
                                                       ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                             >  2:209775/1‑121 (MQ=255)
                                                       ttACCCGATCTGGCGTGGCTTAATACCATTCCGGGGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                             <  1:209775/121‑1 (MQ=255)
                                                               tCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                         >  2:218136/1‑101 (MQ=255)
                                                               tCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                         <  1:218136/101‑1 (MQ=255)
                                                                        gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGACTGGCACAACTTGCTGACTATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTAGTTGGGCAggcggc                                          >  1:1356916/1‑139 (MQ=255)
                                                                                  aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATCTGGGGGGGGACGGCGATCATGGCTAAAGTCCGCCCTTATGTTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCggg                                <  2:869375/139‑1 (MQ=255)
                                                                                   ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                               >  1:510699/1‑139 (MQ=255)
                                                                                        gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                         >  2:396216/1‑76 (MQ=255)
                                                                                        gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                         <  1:396216/76‑1 (MQ=255)
                                                                                        gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCttt                          <  2:176420/139‑1 (MQ=255)
                                                                                             tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATg                                                                                                                    >  1:1061355/1‑43 (MQ=255)
                                                                                             tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATg                                                                                                                    <  2:1061355/43‑1 (MQ=255)
                                                                                              ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATggg                                                                                                                  <  1:698657/44‑1 (MQ=255)
                                                                                              ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATggg                                                                                                                  >  2:698657/1‑44 (MQ=255)
                                                                                                     aGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCt                                                                                   <  2:1296896/69‑1 (MQ=255)
                                                                                                     aGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCt                                                                                   >  1:1296896/1‑69 (MQ=255)
                                                                                                             gCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATg     >  2:1252283/1‑139 (MQ=255)
                                                                                                               aGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGccc                                                                                    >  1:778517/1‑58 (MQ=255)
                                                                                                               aGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGccc                                                                                    <  2:778517/58‑1 (MQ=255)
                                                                                                                ggCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCtt  <  1:70292/139‑1 (MQ=255)
                                                                                                                 gCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGt                     <  1:709092/119‑1 (MQ=255)
                                                                                                                 gCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGt                     >  2:709092/1‑119 (MQ=255)
                                                                                                                 gCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTg                    <  2:645867/120‑1 (MQ=255)
                                                                                                                 gCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTg                    >  1:645867/1‑120 (MQ=255)
                                                                                                                               cTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                        >  1:367610/1‑38 (MQ=255)
                                                                                                                               cTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                        <  2:367610/38‑1 (MQ=255)
                                                                                                                               cTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                    >  1:1135128/1‑58 (MQ=255)
                                                                                                                               cTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                    <  2:1135128/58‑1 (MQ=255)
                                                                                                                                       |                                                                                                                   
TATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTT  >  NC_000913/1769337‑1769586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: