Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,600,609	(C)5→6	coding (132/471 nt)	bcp →	peroxiredoxin, thiol peroxidase, thioredoxin‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,600,604	1	.	C	100.0%	195.1 / NA	54	T43P (ACC→CCC)	bcp	peroxiredoxin, thiol peroxidase, thioredoxin‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (28/26);  total (28/26)

AGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAG  >  NC_000913/2600469‑2600730                                                                                                                                         |                                                                                                                               aGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                             >  1:800186/1‑139 (MQ=255)    tAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑cccccc                                                                                                                          <  2:800186/139‑2 (MQ=255)      aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                             <  2:121960/134‑1 (MQ=255)      aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                             >  1:121960/1‑134 (MQ=255)           tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    >  2:1199653/1‑139 (MQ=255)           tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  2:331392/139‑1 (MQ=255)           tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    >  2:824173/1‑139 (MQ=255)                ccACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                               <  1:1021262/139‑1 (MQ=255)                 cACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTAc                                                                                                              >  1:1088553/1‑139 (MQ=255)                  aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑cccc                                                                                                                            >  2:1307393/1‑123 (MQ=255)                  aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑cccc                                                                                                                            <  1:1307393/123‑1 (MQ=255)                           cGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCgg                                                                                                    >  1:1176841/1‑139 (MQ=255)                             gTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCt                                                                                                  >  1:1180901/1‑139 (MQ=255)                              tGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCtt                                                                                                 >  2:1014466/1‑139 (MQ=255)                               gATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTa                                                                                                <  2:1176841/139‑1 (MQ=255)                               gATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTa                                                                                                <  1:1014466/139‑1 (MQ=255)                                        ccGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAAc                                                                                       >  2:539231/1‑139 (MQ=255)                                           aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                             >  2:712505/1‑114 (MQ=255)                                           aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                             <  1:712505/114‑1 (MQ=255)                                              tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                 <  1:824173/139‑1 (MQ=255)                                                 aGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTg                                                                                                                      >  1:1298502/1‑99 (MQ=255)                                                 aGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTg                                                                                                                      <  2:1298502/99‑1 (MQ=255)                                                 aGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAg                                                                              >  1:856649/1‑139 (MQ=255)                                                        cGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATATCATGGATGAGTTGaaaa                                                                       <  2:856649/139‑1 (MQ=255)                                                            tCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGc                                                                   <  2:385329/139‑1 (MQ=255)                                                                     agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGa                                                          >  2:353705/1‑139 (MQ=255)                                                                         cAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                      <  2:1180901/139‑1 (MQ=255)                                                                            gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                   <  2:51034/139‑1 (MQ=255)                                                                                 tttGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                              >  2:1052096/1‑139 (MQ=255)                                                                                    gACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGc                                                     <  2:40604/129‑1 (MQ=255)                                                                                    gACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGc                                                     >  1:40604/1‑129 (MQ=255)                                                                                     aCCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc                                          <  2:583309/139‑1 (MQ=255)                                                                                       cGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCAc                                        >  2:1289683/1‑139 (MQ=255)                                                                                          cTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg                                                           <  1:778821/117‑1 (MQ=255)                                                                                          cTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg                                                           >  2:778821/1‑117 (MQ=255)                                                                                           ttCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                        <  2:652621/54‑4 (MQ=255)                                                                                           ttCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                        >  1:652621/1‑51 (MQ=255)                                                                                             ccAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATaa                                  <  1:1199653/139‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    >  2:693066/1‑47 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                    <  1:693066/47‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                               <  1:884578/52‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                               >  2:884578/1‑52 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTa                                                                                                <  1:757356/67‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTa                                                                                                >  2:757356/1‑67 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAc                                                                                               <  2:1265655/68‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAc                                                                                               >  1:1265655/1‑68 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                 >  2:215526/1‑82 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                 <  1:215526/82‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGTGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                        >  2:167598/1‑139 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                              <  2:793104/117‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                              >  1:793104/1‑117 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                        <  1:539231/139‑1 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                        >  2:905556/1‑39 (MQ=255)                                                                                                       cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                        <  1:905556/42‑4 (MQ=255)                                                                                                               ggTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCt                                                                                                  >  2:157124/1‑57 (MQ=255)                                                                                                               ggTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCt                                                                                                  <  1:157124/57‑1 (MQ=255)                                                                                                               ggTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCg                >  1:1197406/1‑139 (MQ=255)                                                                                                                  ttATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGttt             <  2:1197406/139‑1 (MQ=255)                                                                                                                     tttCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGc          >  2:85855/1‑139 (MQ=255)                                                                                                                          aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                           >  2:459767/1‑53 (MQ=255)                                                                                                                          aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                           <  1:459767/53‑1 (MQ=255)                                                                                                                          aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGaaa     >  2:46579/1‑139 (MQ=255)                                                                                                                          aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGaaa     >  1:294930/1‑139 (MQ=255)                                                                                                                             cGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACACGATAACACGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAag  <  2:847234/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                               AGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAG  >  NC_000913/2600469‑2600730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑