Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,746	(A)8→9	coding (599/906 nt)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,738	1	.	A	100.0%	179.1 / NA	55	G203C (GGC→TGC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (28/27);  total (28/27)

CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511601‑3511869                                                                                                                                           |                                                                                                                                     cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a                                                                                                                                    >  1:1029947/1‑139 (MQ=255)  ggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aa                                                                                                                                   <  1:859774/139‑1 (MQ=255)                     gCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGa                                                                                                                 >  2:519486/1‑139 (MQ=255)                          gTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTc                                                                                                            >  1:729591/1‑139 (MQ=255)                           tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCt                                                                                                           >  2:1034967/1‑139 (MQ=255)                                   ttttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgcc                                                                                                   >  1:419482/1‑139 (MQ=255)                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:495280/139‑1 (MQ=255)                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  2:265693/139‑1 (MQ=255)                                     ttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGa                                                                                                 >  1:422107/1‑139 (MQ=255)                                      tCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                <  2:419482/139‑1 (MQ=255)                                         gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  1:120879/1‑139 (MQ=255)                                                        aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  1:954920/139‑1 (MQ=255)                                                        aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a                                                                                                                                    <  2:879753/84‑1 (MQ=255)                                                        aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a                                                                                                                                    >  1:879753/1‑84 (MQ=255)                                                            ccGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCt                                                                          <  2:120879/139‑1 (MQ=255)                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a                                                                                                                                    <  2:858564/79‑1 (MQ=255)                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a                                                                                                                                    >  1:858564/1‑79 (MQ=255)                                                               cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCATGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:491559/112‑1 (MQ=255)                                                               cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCATGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:491559/1‑112 (MQ=255)                                                                       tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                               >  1:1020781/1‑139 (MQ=255)                                                                         gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                             <  1:519486/139‑1 (MQ=255)                                                                              aaaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTAGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGCAATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTACACGTTTGCGGACTCGc                                                        >  2:841495/2‑139 (MQ=255)                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCTCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                  <  1:844075/139‑1 (MQ=255)                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aa                                                                                                                                   <  1:286328/57‑1 (MQ=255)                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aa                                                                                                                                   >  2:286328/1‑57 (MQ=255)                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                 >  2:338006/1‑139 (MQ=255)                                                                                                    gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  <  1:1034967/139‑1 (MQ=255)                                                                                                    gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  <  1:338006/139‑1 (MQ=255)                                                                                                     tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:1066695/74‑1 (MQ=255)                                                                                                     tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:1076549/74‑1 (MQ=255)                                                                                                     tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:1076549/1‑74 (MQ=255)                                                                                                     tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:1066695/1‑74 (MQ=255)                                                                                                     tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                                 >  1:571458/1‑139 (MQ=255)                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              >  1:230739/1‑92 (MQ=255)                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                               <  2:571458/139‑1 (MQ=255)                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCCCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                               >  1:260830/1‑139 (MQ=255)                                                                                                       aTAAGAGAAATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  2:230739/92‑1 (MQ=255)                                                                                                         aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  2:1020781/139‑1 (MQ=255)                                                                                                         aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  2:729591/139‑1 (MQ=255)                                                                                                              agaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  2:1236519/134‑1 (MQ=255)                                                                                                              agaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             >  1:1236519/1‑134 (MQ=255)                                                                                                               gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                               >  1:28416/1‑68 (MQ=255)                                                                                                               gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                               <  2:28416/68‑1 (MQ=255)                                                                                                                aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                  <  2:206787/95‑1 (MQ=255)                                                                                                                aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                  >  1:206787/1‑95 (MQ=255)                                                                                                                aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                      >  1:295700/1‑139 (MQ=255)                                                                                                                      tCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                               <  1:548484/92‑1 (MQ=255)                                                                                                                      tCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                               >  2:548484/1‑92 (MQ=255)                                                                                                                             acaTCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcccc         <  2:1029947/139‑1 (MQ=255)                                                                                                                                   cTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  2:633846/49‑1 (MQ=39)                                                                                                                                   cTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  1:633846/1‑49 (MQ=39)                                                                                                                                   cTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                             <  1:1250918/81‑1 (MQ=255)                                                                                                                                   cTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                             >  2:1250918/1‑81 (MQ=255)                                                                                                                                   cTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCtt                                                     >  2:46752/1‑89 (MQ=255)                                                                                                                                   cTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCtt                                                     <  1:46752/89‑1 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  2:231496/1‑48 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  1:231496/48‑1 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                               >  1:1156593/1‑78 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                               <  2:1156593/78‑1 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               <  2:1030165/94‑1 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               >  1:1030165/1‑94 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  1:1193764/1‑139 (MQ=255)                                                                                                                                    tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  2:901338/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                     CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511601‑3511869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑