Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,709,561 (C)6→7 coding (915/1692 nt) eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,709,5551.C95.2% 209.3 / 2.4 62coding (921/1692 nt)eptBKDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (30/29);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATA  >  NC_000913/3709417‑3709684
                                                                                                                                           |                                                                                                                                  
aGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                     <  2:419209/139‑1 (MQ=255)
aGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                     <  2:1217845/139‑1 (MQ=255)
       ttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccc                                                                                                                            >  2:806370/1‑138 (MQ=255)
        tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccct                                                                                                                           <  1:251039/139‑3 (MQ=255)
         attcacaaTCGTGTTGCTGTATAACCTCATTTCGCTCTGCATAGGCTAGAGGTCAAAACTGAATCCTAACTGCTTCCGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCAGCGGATTATCTTCCGCGccccccctg                                                                                                                          <  1:982296/132‑4 (MQ=255)
            cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACg                                                                                                                        <  2:1097772/139‑1 (MQ=255)
            cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACg                                                                                                                        <  1:206852/139‑1 (MQ=255)
              ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTAc                                                                                                                     <  2:1149381/139‑1 (MQ=255)
               cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTAc                                                                                                                     >  2:56250/1‑139 (MQ=255)
               cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTAc                                                                                                                     >  1:1279019/1‑139 (MQ=255)
                 tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                   <  1:806370/139‑1 (MQ=255)
                           gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACg                                                                                                         <  2:271187/139‑1 (MQ=255)
                            tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGc                                                                                                        <  1:376150/139‑1 (MQ=255)
                              gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCTGCGCCCCCCCTGACGTACAAACATGCAACGCAg                                                                                                      >  1:620637/1‑139 (MQ=255)
                              gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAg                                                                                                      >  2:308089/1‑139 (MQ=255)
                              gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAg                                                                                                      >  2:507558/1‑139 (MQ=255)
                               aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                     <  2:1199583/139‑1 (MQ=255)
                               aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                     <  1:945289/139‑1 (MQ=255)
                                    caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGagag                                                                                                >  2:1357383/1‑139 (MQ=255)
                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                     <  2:129456/101‑1 (MQ=255)
                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                     >  1:129456/1‑101 (MQ=255)
                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAAc                                                                                                          >  2:1265684/1‑127 (MQ=255)
                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAAc                                                                                                          <  1:1265684/127‑1 (MQ=255)
                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcc                                                                                                                                 <  2:1079555/103‑1 (MQ=255)
                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcc                                                                                                                                 >  1:1079555/1‑103 (MQ=255)
                                             ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                       <  2:794229/139‑1 (MQ=255)
                                               gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          >  2:1063739/1‑98 (MQ=255)
                                               gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          <  1:1063739/101‑4 (MQ=255)
                                               gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccccc                                                                                                                             >  1:568694/1‑98 (MQ=255)
                                               gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccccc                                                                                                                             <  2:568694/98‑1 (MQ=255)
                                                cATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGt                                                                                    >  1:332723/1‑139 (MQ=255)
                                                    gCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCTCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                <  1:83947/139‑1 (MQ=255)
                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                       <  1:36679/126‑1 (MQ=255)
                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                       >  2:36679/1‑126 (MQ=255)
                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                          >  1:377597/1‑139 (MQ=255)
                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          <  2:1154652/90‑4 (MQ=255)
                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          >  1:1154652/1‑87 (MQ=255)
                                                          agGCCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTACCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                          >  1:879615/1‑139 (MQ=255)
                                                              cAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                >  1:443335/1‑129 (MQ=255)
                                                              cAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                <  2:443335/129‑1 (MQ=255)
                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccccc                                                                                                                             >  2:765448/1‑82 (MQ=255)
                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccccc                                                                                                                             <  1:765448/82‑1 (MQ=255)
                                                                    tGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa                                                                >  2:826383/1‑139 (MQ=255)
                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                                                              >  2:1141041/1‑139 (MQ=255)
                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                                                              >  1:917832/1‑139 (MQ=255)
                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                                                              >  1:828648/1‑139 (MQ=255)
                                                                             aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa                                                       >  2:286957/1‑139 (MQ=255)
                                                                                gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                   <  2:219621/76‑1 (MQ=255)
                                                                                gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                   >  1:219621/1‑76 (MQ=255)
                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt                                                  >  2:675221/1‑139 (MQ=255)
                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          <  1:1160880/66‑4 (MQ=255)
                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          >  2:1160880/1‑63 (MQ=255)
                                                                                   tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtttt                                                 >  2:190689/1‑139 (MQ=255)
                                                                                    cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                   <  2:1136234/72‑1 (MQ=255)
                                                                                    cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                   >  1:1136234/1‑72 (MQ=255)
                                                                                        aCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc                                            <  2:1279019/139‑1 (MQ=255)
                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt                                           <  2:879615/139‑1 (MQ=255)
                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          <  2:1205763/59‑4 (MQ=255)
                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          >  1:1205763/1‑56 (MQ=255)
                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccccc                                                                                                                             >  1:648849/1‑53 (MQ=255)
                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccccc                                                                                                                             <  2:648849/53‑1 (MQ=255)
                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTCCCGTGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                        >  1:507850/1‑139 (MQ=255)
                                                                                             gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                       <  1:675221/139‑1 (MQ=255)
                                                                                                        gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                            <  2:377597/139‑1 (MQ=255)
                                                                                                        gttctTTTAATGTGCGCTGCGGATTATCTCCCGTGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                            <  2:507850/139‑1 (MQ=255)
                                                                                                         ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACaa                                                                                                                   >  1:1061625/1‑50 (MQ=255)
                                                                                                         ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACaa                                                                                                                   <  2:1061625/50‑1 (MQ=255)
                                                                                                              ttAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          <  2:1243054/38‑4 (MQ=255)
                                                                                                              ttAATGTGCGCTGCGGATTATCTTCCGCGccccccctg                                                                                                                          >  1:1243054/1‑35 (MQ=255)
                                                                                                                 aTGTGTGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGGGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTTTCCTGGGCCAGTTTCGGCGTGGTATTAc                   <  2:848162/139‑1 (MQ=255)
                                                                                                                   gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa                                                                                                           <  1:614704/49‑1 (MQ=255)
                                                                                                                   gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa                                                                                                           >  2:614704/1‑49 (MQ=255)
                                                                                                                   gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                 <  1:363005/123‑1 (MQ=255)
                                                                                                                   gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                 >  2:363005/1‑123 (MQ=255)
                                                                                                                    tgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGtt                                                                                         >  1:796659/1‑66 (MQ=255)
                                                                                                                    tgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGtt                                                                                         <  2:796659/66‑1 (MQ=255)
                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACg                                                                                                         <  1:1198582/48‑1 (MQ=255)
                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACg                                                                                                         >  2:1198582/1‑48 (MQ=255)
                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt                                                  >  2:651966/1‑103 (MQ=255)
                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt                                                  <  1:651966/103‑1 (MQ=255)
                                                                                                                      cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa              >  1:341952/1‑139 (MQ=255)
                                                                                                                         tGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAg           <  1:1357383/139‑1 (MQ=255)
                                                                                                                               ttATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaa     <  1:826383/139‑1 (MQ=255)
                                                                                                                                tATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa    >  1:174805/1‑139 (MQ=255)
                                                                                                                                  tCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCa                                                                                                       <  1:1348826/38‑1 (MQ=255)
                                                                                                                                  tCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCa                                                                                                       >  2:1348826/1‑38 (MQ=255)
                                                                                                                                  tCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATa  <  2:828648/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                  
AGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATA  >  NC_000913/3709417‑3709684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: