| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 1,265,001 | 1086 bp→GAGG | [prfA] | [prfA] | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 1265001 | 1266086 | 1086 | 32 [0] | [0] 32 | [prfA] | [prfA] |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1265000 | 0 (0.000) | 32 (0.620) +GAGG |
26/200 | 0.8 | 100% | intergenic (+30/‑12) | hemA/prfA | glutamyl tRNA reductase/peptide chain release factor RF‑1 |
| ? | NC_000913 | 1266087 = | 0 (0.000) | coding (1076/1083 nt) | prfA | peptide chain release factor RF‑1 | |||||
GCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTT > NC_000913/1265950‑1266218 | taaccgcttgatccatgcgccaacgaaatcacttcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggag < 2:473214‑M2/2‑1 (MQ=255)taaccgcttgatccatgcgccaacgaaatcacttcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggag < 1:310348‑M2/2‑1 (MQ=255) tgcgccaacgaaatcacttcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATc > 1:929954‑M2/123‑139 (MQ=255) tgcgccaacgaaatcacttcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATc > 2:554215‑M2/123‑139 (MQ=255) aacgaaatcacttcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATgg < 2:1266274‑M2/11‑1 (MQ=255) aacgaaatcacttcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATgg > 1:1266274‑M2/117‑127 (MQ=255) tcaacaggccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAg > 1:703317‑M2/105‑139 (MQ=255) ccgcccgtgatggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGc < 2:929954‑M2/43‑1 (MQ=255) cgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGccc > 1:633989‑M2/74‑139 (MQ=255) cctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcgc > 2:558463‑M2/72‑139 (MQ=255) aatattctgcgcgacagcctcgggctggagtaacagtacttcattttctttttttacagggtggagaAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGTGAAAGCCCgcggcg < 2:831124‑M2/72‑1 (MQ=255) aatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATgg > 1:479124‑M2/68‑78 (MQ=255) aatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATgg < 2:479124‑M2/11‑1 (MQ=255) aatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg < 1:554215‑M2/72‑1 (MQ=255) attctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGa < 1:558463‑M2/75‑1 (MQ=255) gcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATc < 1:1273267‑M2/17‑1 (MQ=255) gcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATc > 2:1273267‑M2/58‑74 (MQ=255) agtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGtt > 2:822126‑M2/40‑139 (MQ=255) cagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCgg > 2:1010616‑M2/35‑139 (MQ=255) agtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGc < 1:1010616‑M2/106‑1 (MQ=255) acatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg < 2:105527‑M2/72‑1 (MQ=255) acatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg > 1:105527‑M2/31‑102 (MQ=255) catcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCagag > 2:1228238‑M2/30‑139 (MQ=255) catcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCagag > 2:1050177‑M2/30‑139 (MQ=255) tttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGcc > 2:121594‑M2/24‑67 (MQ=255) tttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGcc < 1:121594‑M2/44‑1 (MQ=255) tacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGa < 2:1046543‑M2/33‑1 (MQ=255) tacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGa > 1:1046543‑M2/14‑46 (MQ=255) gggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGaaa < 1:690233‑M2/83‑1 (MQ=255) gggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGaaa > 2:690233‑M2/10‑92 (MQ=255) gggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGcc < 1:822126‑M2/130‑1 (MQ=255) gtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCtt < 1:1228238‑M2/132‑1 (MQ=255) | GCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTT > NC_000913/1265950‑1266218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |