Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,721	C→A	M143I (ATG→ATT)	ybbO ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,721	0	C	A	100.0%	54.4 / NA	18	M143I (ATG→ATT)	ybbO	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/10);  total (8/10)

TTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGG  >  NC_000913/518586‑518847                                                                                                                                        |                                                                                                                               ttCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtaatc                                                                                                                             <  1:145260/139‑1 (MQ=255)                tttAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCa                                                                                                              <  2:231152/139‑1 (MQ=255)                 ttAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCAc                                                                                                             <  1:1233278/139‑1 (MQ=255)                   aaTTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                           <  2:1280445/139‑1 (MQ=255)                          cTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTa                                                                                                    >  1:542134/1‑139 (MQ=255)                                               aGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCa                                                                               >  2:157824/1‑139 (MQ=255)                                                                          tatTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGt                                                    >  1:390007/1‑139 (MQ=255)                                                                                  ggCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa                                                                                     >  2:598849/1‑98 (MQ=255)                                                                                  ggCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa                                                                                     <  1:598849/98‑1 (MQ=255)                                                                                     cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATa                                                                                                                       >  1:273872/1‑61 (MQ=255)                                                                                     cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATa                                                                                                                       <  2:273872/61‑1 (MQ=255)                                                                                           aGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGt                                   <  2:390007/139‑1 (MQ=255)                                                                                             cgcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGtgtg                                                                >  2:827161/1‑108 (MQ=255)                                                                                             cgcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGtgtg                                                                <  1:827161/108‑1 (MQ=255)                                                                                                                            cACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAggggg  <  2:1275424/139‑1 (MQ=255)                                                                                                                                 atgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                >  2:332288/1‑120 (MQ=255)                                                                                                                                 atgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                <  1:332288/120‑1 (MQ=255)                                                                                                                                  tgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTg                                                  >  2:572840/1‑85 (MQ=255)                                                                                                                                  tgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTg                                                  <  1:572840/85‑1 (MQ=255)                                                                                                                                        |                                                                                                                               TTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGG  >  NC_000913/518586‑518847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑