Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 522,463 T→C V683A (GTG→GCG)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913522,4630TC100.0% 148.4 / NA 42V683A (GTG→GCG) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

GTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACC  >  NC_000913/522328‑522593
                                                                                                                                       |                                                                                                                                  
gTTTCCGCTGGTAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTAGAAGCGAt                                                                                                                                 <  2:888749/139‑1 (MQ=255)
  ttCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                               <  2:1171929/139‑1 (MQ=255)
  ttCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                               >  2:364975/1‑139 (MQ=255)
         gggAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACt                                                                                                                        >  2:772519/1‑139 (MQ=255)
               aTGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCAc                                                                                                                  <  1:364975/139‑1 (MQ=255)
                     aCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTg                                                                                                            <  2:255860/139‑1 (MQ=255)
                                 cacaACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                <  2:1369394/139‑1 (MQ=255)
                                      cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                           >  2:79269/1‑139 (MQ=255)
                                      cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                           >  1:1175933/1‑139 (MQ=255)
                                      cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                           >  2:1336443/1‑139 (MQ=255)
                                           ccGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGca                                                                                      <  2:1175933/139‑1 (MQ=255)
                                              ccAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAt                                                                                                                                 <  2:481076/93‑1 (MQ=255)
                                              ccAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAt                                                                                                                                 >  1:481076/1‑93 (MQ=255)
                                                 gTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAt                                                                                                      <  1:1333930/117‑1 (MQ=255)
                                                 gTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAt                                                                                                      >  2:1333930/1‑117 (MQ=255)
                                                 gTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                >  2:24435/1‑139 (MQ=255)
                                                                  tGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtca                                                               <  1:688821/139‑1 (MQ=255)
                                                                    ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagc                                                             >  2:105787/1‑139 (MQ=255)
                                                                          atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTa                                                                                                                          >  1:657925/1‑72 (MQ=255)
                                                                          atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTa                                                                                                                          <  2:657925/72‑1 (MQ=255)
                                                                              gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCg                                                                                                          >  1:1231293/1‑84 (MQ=255)
                                                                              gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCg                                                                                                          <  2:1231293/84‑1 (MQ=255)
                                                                              gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCg                                                                                                          >  2:397445/1‑84 (MQ=255)
                                                                              gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCg                                                                                                          <  1:397445/84‑1 (MQ=255)
                                                                              gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                   >  1:716575/1‑139 (MQ=255)
                                                                                        aaaaCAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGgtggtg                                         <  2:716575/139‑1 (MQ=255)
                                                                                              ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCG‑TGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                            >  2:1010441/1‑113 (MQ=255)
                                                                                              ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCG‑TGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                            <  1:1010441/113‑1 (MQ=255)
                                                                                               gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAt                                                                                                                                 <  2:482947/44‑1 (MQ=255)
                                                                                               gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAt                                                                                                                                 >  1:482947/1‑44 (MQ=255)
                                                                                                   gtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                              <  1:401908/139‑1 (MQ=255)
                                                                                                       ggTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGt                          <  1:772519/139‑1 (MQ=255)
                                                                                                         tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCAcc                                                                                                                 >  2:633404/1‑50 (MQ=255)
                                                                                                         tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCAcc                                                                                                                 <  1:633404/50‑1 (MQ=255)
                                                                                                         tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCgg                                                                                                         >  1:1280488/1‑58 (MQ=255)
                                                                                                         tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCgg                                                                                                         <  2:1280488/58‑1 (MQ=255)
                                                                                                         tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                       >  2:1003142/1‑76 (MQ=255)
                                                                                                         tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                       <  1:1003142/76‑1 (MQ=255)
                                                                                                             ggAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCgggg                    >  1:323270/1‑139 (MQ=255)
                                                                                                              gAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGa                   >  1:737169/1‑139 (MQ=255)
                                                                                                                         tCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgctg        >  2:247251/1‑139 (MQ=255)
                                                                                                                               gcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc  <  1:247251/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACC  >  NC_000913/522328‑522593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑