Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,614	T→A	intergenic (‑64/+221)	araD ← / ← araA	L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,614	0	T	A	100.0%	50.5 / NA	20	intergenic (‑64/+221)	araD/araA	L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/9);  total (11/9)

GCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTAT  >  NC_000913/66481‑66669                                                                                                                                       |                                                        gCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATaa                                                    >  2:489955/1‑139 (MQ=255)  cGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAg                                                   >  2:811188/1‑139 (MQ=255)    tGACCAGGTTGTGTTTTGGCAGCGCCAGGATGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGCCTCCTTCGTGCCGGATGCGCTTTTCTTATCCAGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGAc                                                 >  1:915861/1‑139 (MQ=37)         cggTTTTGTTTTTGGAGGGCCAAGTTGGCTTCTAATACCCTGGCTTTTGGGAT‑TTCTAACATGTTTACTTCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGAcgcgtc                                            <  1:595604/138‑3 (MQ=17)              gtgtTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGt                                       <  1:1342506/139‑1 (MQ=255)                  tttGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCa                                   <  2:519777/139‑1 (MQ=255)                   ttGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCAGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCAt                                  <  2:582706/139‑1 (MQ=255)                                     cTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGttacataccgga                <  2:737968/139‑11 (MQ=17)                                         tAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCa                                   <  1:808854/116‑1 (MQ=255)                                         tAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCa                                   >  2:808854/1‑116 (MQ=255)                                          aaTA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATgcgg           >  1:359728/1‑138 (MQ=17)                                               ccTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATgcggctac       >  2:496269/1‑134 (MQ=14)                                                cTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATgcggctact      >  1:208828/1‑133 (MQ=14)                                                  ggCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCAt                                  <  1:293570/109‑1 (MQ=255)                                                  ggCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCAt                                  >  2:293570/1‑109 (MQ=255)                                                  ggCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATc                                 >  1:1333348/1‑110 (MQ=255)                                                  ggCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATc                                 <  2:1333348/110‑1 (MQ=255)                                                   gCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATgcggctacttag   <  1:1179442/139‑10 (MQ=11)                                                    cGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATgcggctacttagc  >  2:521143/1‑129 (MQ=11)                                                    cGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATgcggctacttagc  >  2:896532/1‑129 (MQ=11)                                                                                                                                       |                                                        GCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTAT  >  NC_000913/66481‑66669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑