Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 829,972 C→T M1M (GTG→ATG) †
*224* (TAG→TAA
ybhG ←
ybiH ←
putative membrane fusion protein (MFP) component of efflux pump, membrane anchor
DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913829,9720CT100.0% 112.0 / NA 35M1M (GTG→ATG) †
*224* (TAG→TAA
ybhG
ybiH
putative membrane fusion protein (MFP) component of efflux pump, membrane anchor
DUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

CGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAG  >  NC_000913/829845‑830103
                                                                                                                               |                                                                                                                                   
cGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                          >  1:1069325/1‑139 (MQ=255)
      tCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGa                                                                                                                    <  1:167184/139‑1 (MQ=255)
                 aTACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCCTTACAAACTCCTTTGCGATAATCCTTGCa                                                                                                         <  1:789550/139‑1 (MQ=255)
                       cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                                   >  1:251056/1‑139 (MQ=255)
                        gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                  <  1:377399/139‑1 (MQ=255)
                        gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                  <  2:251056/139‑1 (MQ=255)
                         tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                                 >  2:296987/1‑139 (MQ=255)
                         tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                                 >  1:1129383/1‑139 (MQ=255)
                            ggCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCg                                                                                              >  2:332764/1‑139 (MQ=255)
                                            gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                              <  1:296987/139‑1 (MQ=255)
                                            gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                              >  2:1252799/1‑139 (MQ=255)
                                                          aGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                          >  2:514406/1‑81 (MQ=255)
                                                          aGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                          <  1:514406/81‑1 (MQ=255)
                                                                cTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                   >  1:215786/1‑114 (MQ=255)
                                                                cTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                   <  2:215786/114‑1 (MQ=255)
                                                                 tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCt                                                         <  1:1252799/139‑1 (MQ=255)
                                                                  ccGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                                    <  1:844992/95‑1 (MQ=255)
                                                                  ccGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                                    >  2:844992/1‑95 (MQ=255)
                                                                   cGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                  >  2:340756/1‑96 (MQ=255)
                                                                   cGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                  <  1:340756/96‑1 (MQ=255)
                                                                          cACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                   >  2:400294/1‑104 (MQ=255)
                                                                          cACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                   <  1:400294/104‑1 (MQ=255)
                                                                             ggcggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                                >  2:865095/1‑88 (MQ=255)
                                                                             ggcggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                                <  1:865095/88‑1 (MQ=255)
                                                                             ggcggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGa                                             >  2:270124/1‑139 (MQ=255)
                                                                                    aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACa                                                                                                                                 >  1:40979/1‑48 (MQ=255)
                                                                                    aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACa                                                                                                                                 <  2:40979/48‑1 (MQ=255)
                                                                                         cACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                 <  1:332764/139‑1 (MQ=255)
                                                                                                   aaTCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTat                       >  1:1090787/1‑138 (MQ=255)
                                                                                                         aTCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAt                 >  1:492502/1‑139 (MQ=255)
                                                                                                          tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                 <  2:1113165/122‑1 (MQ=255)
                                                                                                          tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                 >  1:722685/1‑122 (MQ=255)
                                                                                                          tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                 <  2:722685/122‑1 (MQ=255)
                                                                                                          tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                 >  1:1113165/1‑122 (MQ=255)
                                                                                                                        ttCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAg  >  2:788927/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                   
CGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAG  >  NC_000913/829845‑830103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: