Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	860,638	A→G	Y657H (TAT→CAT)	ybiW ←	putative pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	860,638	0	A	G	100.0%	198.1 / NA	55	Y657H (TAT→CAT)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

CAAGATGGTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCG  >  NC_000913/860506‑860768                                                                                                                                     |                                                                                                                                   cAAGATGGTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTgcc                                                                                                                              <  1:1281191/139‑1 (MQ=255)     aTGGTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCa                                                                                                                          >  2:122004/1‑139 (MQ=255)             tACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGAcc                                                                                                                  >  1:485645/1‑139 (MQ=255)               ccGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCAc                                                                                                                <  1:303980/139‑1 (MQ=255)                  gAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGAc                                                                                                             >  1:588297/1‑139 (MQ=255)                  gAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGAc                                                                                                             >  1:47632/1‑139 (MQ=255)                       cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGAc                                                                                                                   >  1:7537/1‑128 (MQ=255)                       cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGAc                                                                                                                   <  2:7537/128‑1 (MQ=255)                       cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAg                                                                                                        >  1:1037443/1‑139 (MQ=255)                       cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAg                                                                                                        >  2:181707/1‑139 (MQ=255)                       cGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAg                                                                                                        >  1:1197651/1‑139 (MQ=255)                         ggCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgcg                                                                                                      <  2:380622/139‑1 (MQ=255)                         ggCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgcg                                                                                                      <  1:122004/139‑1 (MQ=255)                           cTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg                                                                                                    <  2:588297/139‑1 (MQ=255)                           cTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg                                                                                                    <  1:1314061/139‑1 (MQ=255)                           cTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg                                                                                                    <  1:307574/139‑1 (MQ=255)                                                   tgGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                            <  1:145078/139‑1 (MQ=255)                                                   tgGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt                                                                            <  2:1197651/139‑1 (MQ=255)                                                        tttACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                       <  2:1037443/139‑1 (MQ=255)                                                          tACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGAtata                                                                     <  2:47632/139‑1 (MQ=255)                                                                       tgtTGCCGTAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcg                                                        <  2:407721/139‑1 (MQ=255)                                                                            ccATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg                                                                                                    >  2:359726/1‑90 (MQ=255)                                                                            ccATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg                                                                                                    <  1:359726/90‑1 (MQ=255)                                                                                aGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagc                                               <  2:921868/139‑1 (MQ=255)                                                                                    tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCg                                                                                                                      >  2:385109/1‑64 (MQ=255)                                                                                    tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCg                                                                                                                      <  1:385109/64‑1 (MQ=255)                                                                                    tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                       >  2:903694/1‑111 (MQ=255)                                                                                    tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                       <  1:903694/111‑1 (MQ=255)                                                                                      cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCAc                                                                                                                <  1:1063013/68‑1 (MQ=255)                                                                                      cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCAc                                                                                                                >  2:1063013/1‑68 (MQ=255)                                                                                      cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                         >  1:1216021/1‑139 (MQ=255)                                                                                      cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                         >  1:470597/1‑139 (MQ=255)                                                                                      cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa                                         >  2:1309472/1‑139 (MQ=255)                                                                                       gcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTAt                                        >  1:891853/1‑139 (MQ=255)                                                                                          ccAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa                                     <  2:470597/139‑1 (MQ=255)                                                                                                        aGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt                                                             >  1:104718/1‑101 (MQ=255)                                                                                                        aGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt                                                             <  2:104718/101‑1 (MQ=255)                                                                                                              gaTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg                                                                             >  2:770442/1‑79 (MQ=255)                                                                                                              gaTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg                                                                             <  1:770442/79‑1 (MQ=255)                                                                                                              gaTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg                 >  1:309227/1‑139 (MQ=255)                                                                                                                   atgaCGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACtt            >  2:842127/1‑139 (MQ=255)                                                                                                                   atgaCGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACtt            >  1:134830/1‑139 (MQ=255)                                                                                                                   atgaCGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACtt            <  1:181707/139‑1 (MQ=255)                                                                                                                        gTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg       <  2:485645/139‑1 (MQ=255)                                                                                                                         tACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                       >  2:1335858/1‑74 (MQ=255)                                                                                                                         tACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata                                                                       <  1:1335858/74‑1 (MQ=255)                                                                                                                          aCCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc                                       <  1:994135/105‑1 (MQ=255)                                                                                                                          aCCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc                                       >  2:994135/1‑105 (MQ=255)                                                                                                                             cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGAt                                                                                                 >  1:783392/1‑44 (MQ=255)                                                                                                                             cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGAt                                                                                                 <  2:783392/44‑1 (MQ=255)                                                                                                                             cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGAt                                                                                                 <  2:1105036/44‑1 (MQ=255)                                                                                                                             cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGAt                                                                                                 >  1:1105036/1‑44 (MQ=255)                                                                                                                             cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCg  >  1:401271/1‑139 (MQ=255)                                                                                                                               cgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgt                         >  2:713060/1‑114 (MQ=255)                                                                                                                               cgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgt                         <  1:713060/114‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CAAGATGGTCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCG  >  NC_000913/860506‑860768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑