Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 911,132 A→G intergenic (‑83/+50) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913911,1320AG100.0% 107.4 / NA 31intergenic (‑83/+50)poxB/hcrpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

GATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATT  >  NC_000913/911006‑911266
                                                                                                                              |                                                                                                                                      
gATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa                                                                                                                            <  1:1289660/139‑1 (MQ=255)
                           tcAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAAAAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg                                                                                                 <  1:557442/138‑1 (MQ=255)
                             aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                                                               >  2:201822/1‑139 (MQ=255)
                                 gtttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                           <  2:1188648/139‑1 (MQ=255)
                                  tttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAgg                                                                                          >  2:416132/1‑139 (MQ=255)
                                            ggTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCg                                                                                <  1:295355/139‑1 (MQ=255)
                                               tctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                                                               <  2:20315/121‑1 (MQ=255)
                                               tctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                                                               >  1:20315/1‑121 (MQ=255)
                                                      ctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg                                                                                                                                  >  1:305930/1‑79 (MQ=255)
                                                      ctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg                                                                                                                                  <  2:305930/79‑1 (MQ=255)
                                                        ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATcc                                                                    >  2:970703/1‑139 (MQ=255)
                                                                 gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                           >  2:158532/1‑107 (MQ=255)
                                                                 gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                           <  1:158532/107‑1 (MQ=255)
                                                                  aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa                                                                                                                                    >  1:1056701/1‑65 (MQ=255)
                                                                  aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa                                                                                                                                    <  2:1056701/65‑1 (MQ=255)
                                                                              aGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa                                                <  1:686736/137‑1 (MQ=255)
                                                                              aGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa                                                >  2:686736/1‑137 (MQ=255)
                                                                                 ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa                                           >  1:440926/1‑139 (MQ=255)
                                                                                  tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa                                                                                                                                    <  1:657347/49‑1 (MQ=255)
                                                                                  tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa                                                                                                                                    >  2:657347/1‑49 (MQ=255)
                                                                                           tGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGTACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAcc                                 <  1:970703/139‑1 (MQ=255)
                                                                                                 aGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGc                           <  1:201822/139‑1 (MQ=255)
                                                                                                  gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg                                                                                                 >  2:1177540/1‑68 (MQ=255)
                                                                                                  gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg                                                                                                 <  1:1177540/68‑1 (MQ=255)
                                                                                                   ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc                                                                                                                       >  1:836161/1‑45 (MQ=255)
                                                                                                   ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc                                                                                                                       <  2:836161/45‑1 (MQ=255)
                                                                                                                   agaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAg         <  1:416132/139‑1 (MQ=255)
                                                                                                                    gaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGc        >  2:785325/1‑139 (MQ=255)
                                                                                                                     aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                           <  2:199332/55‑1 (MQ=255)
                                                                                                                     aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                           >  1:199332/1‑55 (MQ=255)
                                                                                                                          aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCAtt  >  2:1349267/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                      
GATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATT  >  NC_000913/911006‑911266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: