Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 104,352 A→G E124G (GAG→GGG)  ftsA → ATP‑binding cell division FtsK recruitment protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913104,3520AG100.0% 172.4 / NA 48E124G (GAG→GGG) ftsAATP‑binding cell division FtsK recruitment protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

TATATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATG  >  NC_000913/104217‑104482
                                                                                                                                       |                                                                                                                                  
tataTCTGGCGCTTTCTGGTAAGCACAACAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCa                                                                                                                                 >  1:12187/1‑139 (MQ=255)
     cTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                            >  1:1178862/1‑139 (MQ=255)
         cgcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgct                                                                                                                        >  1:796955/1‑139 (MQ=255)
          gcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgctg                                                                                                                       >  2:741135/1‑139 (MQ=255)
          gcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgctg                                                                                                                       >  1:601687/1‑139 (MQ=255)
                 ggTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                <  2:983367/139‑1 (MQ=255)
                 ggTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                <  1:33871/139‑1 (MQ=255)
                       cacaTCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGc                                                                                                          <  2:796955/139‑1 (MQ=255)
                            cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                            <  2:1237068/116‑1 (MQ=255)
                            cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                            >  1:1237068/1‑116 (MQ=255)
                            cAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAagag                                                                                                     >  1:473397/1‑139 (MQ=255)
                             aggctccAGAATGAAATTGGTATGGTACCTTTTTCCGAAGAAGGAGTGACGCAAAAAGATTTGGAAAAACTCGTCCCTACCCGGAAATCGGTGGCTGGGTGCGATGGGGCTCCGGGGGCCCAAGTGGTCCCGCAAGAGt                                                                                                    <  1:489655/134‑1 (MQ=255)
                             aGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGt                                                                                                    <  2:36861/139‑1 (MQ=255)
                                               ggTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                  <  2:1058152/139‑1 (MQ=255)
                                                gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa                                                                                 >  1:1065343/1‑139 (MQ=255)
                                                gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa                                                                                 >  2:1165471/1‑139 (MQ=255)
                                                gTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa                                                                                 >  1:318496/1‑139 (MQ=255)
                                                  aTGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                           <  1:214406/111‑1 (MQ=255)
                                                  aTGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCg                                                                                                           >  2:214406/1‑111 (MQ=255)
                                                        ccTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCa                                                                         >  2:256538/1‑139 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                  >  1:1196986/1‑72 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                  <  1:367499/88‑1 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                  >  2:367499/1‑88 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCaa                                                                                                        >  1:553644/1‑98 (MQ=255)
                                                                  aagaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCaa                                                                                                        <  2:553644/98‑1 (MQ=255)
                                                                   agaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                              <  2:473397/139‑1 (MQ=255)
                                                                   agaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                              <  1:741135/139‑1 (MQ=255)
                                                                             aCGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          >  1:629580/1‑69 (MQ=255)
                                                                             aCGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                          <  2:629580/69‑1 (MQ=255)
                                                                             aCGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGCGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCgg                                                    <  1:1165471/139‑1 (MQ=255)
                                                                               gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                >  1:114438/1‑109 (MQ=255)
                                                                               gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                <  2:114438/109‑1 (MQ=255)
                                                                                     agaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCgg                                            >  2:737170/1‑139 (MQ=255)
                                                                                          tgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATc                                                                                                                               >  1:28400/1‑51 (MQ=255)
                                                                                          tgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATc                                                                                                                               <  2:28400/51‑1 (MQ=255)
                                                                                             aaaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                >  1:1202830/1‑63 (MQ=255)
                                                                                             aaaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                <  2:1202830/63‑1 (MQ=255)
                                                                                             aaaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGc                                    >  2:261562/1‑139 (MQ=255)
                                                                                               aaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa                                  <  1:256538/139‑1 (MQ=255)
                                                                                                  gtcgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg                               <  1:737170/139‑1 (MQ=255)
                                                                                                    cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTg                             >  2:1220163/1‑139 (MQ=255)
                                                                                                     gtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGc                            <  2:12187/139‑1 (MQ=255)
                                                                                                       ccATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAc                          <  1:261562/139‑1 (MQ=255)
                                                                                                            ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGAt                     >  1:243327/1‑139 (MQ=255)
                                                                                                               cgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcac                  >  2:1231539/1‑139 (MQ=255)
                                                                                                                 aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACAt                <  2:154456/139‑1 (MQ=255)
                                                                                                                 aaaTCGGTGCGTGAGCGCGATGGGCACCGTCTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGAACTTTCGGGCGTGCGGATGCAGTCAAAAGTGCACCTGATCACAt                <  1:969221/139‑1 (MQ=255)
                                                                                                                     cGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGa                                                                                          <  1:786725/61‑1 (MQ=255)
                                                                                                                     cGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGa                                                                                          >  2:786725/1‑61 (MQ=255)
                                                                                                                               gcgcGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATg  <  2:1178862/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
TATATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATG  >  NC_000913/104217‑104482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑