Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,661	T→C	M22V (ATG→GTG)	hofC ←	assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,661	0	T	C	100.0%	129.7 / NA	37	M22V (ATG→GTG)	hofC	assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/18);  total (19/18)

GCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGC  >  NC_000913/115545‑115787                                                                                                                     |                                                                                                                               gcTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                          <  2:760097/139‑1 (MQ=255)                      cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                     <  1:1097692/123‑1 (MQ=255)                      cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                     >  2:1097692/1‑123 (MQ=255)                      cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgcgc                                                                                     <  2:1306797/139‑1 (MQ=255)                         aGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCag                                                                                  >  1:48667/1‑139 (MQ=255)                                     gATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatg                                                                                                 >  1:1291756/1‑112 (MQ=255)                                     gATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatg                                                                                                 <  2:1291756/112‑1 (MQ=255)                                     gATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTc                                                                      >  1:169875/1‑139 (MQ=255)                                      aTTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACt                                                                       >  1:1175978/1‑137 (MQ=255)                                      aTTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACt                                                                       <  2:1175978/137‑1 (MQ=255)                                               aGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                            <  2:48667/139‑1 (MQ=255)                                                           gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgcgc                                                                                     >  2:213433/1‑102 (MQ=255)                                                           gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgcgc                                                                                     <  1:213433/102‑1 (MQ=255)                                                           gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCg                                                              <  2:39930/125‑1 (MQ=255)                                                           gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCg                                                              >  1:39930/1‑125 (MQ=255)                                                             aaCCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                                >  2:783248/1‑137 (MQ=255)                                                             aaCCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                                <  1:783248/137‑1 (MQ=255)                                                               ccATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATGGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGa                                            >  1:126624/1‑139 (MQ=255)                                                                   cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGt                                                                               <  2:885287/100‑1 (MQ=255)                                                                   cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGt                                                                               >  1:885287/1‑100 (MQ=255)                                                                             aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                              <  2:169875/139‑1 (MQ=255)                                                                                 ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                 <  1:464206/100‑1 (MQ=255)                                                                                 ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                 >  2:464206/1‑100 (MQ=255)                                                                                    tAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatg                                                                                                 >  2:787698/1‑65 (MQ=255)                                                                                    tAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatg                                                                                                 <  1:787698/65‑1 (MQ=255)                                                                                         aGTAAAGTACGGCTCTCTGCCCATGGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCt                  <  2:126624/139‑1 (MQ=255)                                                                                         aGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                          <  1:840520/51‑1 (MQ=255)                                                                                         aGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                          >  2:840520/1‑51 (MQ=255)                                                                                         aGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCt                  >  2:318385/1‑139 (MQ=255)                                                                                                tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                     <  2:647994/49‑1 (MQ=255)                                                                                                tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                     >  1:647994/1‑49 (MQ=255)                                                                                                  cGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGcc                                                                                    >  2:483850/1‑64 (MQ=255)                                                                                                  cGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGcc                                                                                    <  1:483850/64‑1 (MQ=255)                                                                                                   ggCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGCGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                  >  1:564961/1‑97 (MQ=255)                                                                                                   ggCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                  <  2:564961/97‑1 (MQ=255)                                                                                                   ggCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACgg        >  1:894134/1‑139 (MQ=255)                                                                                                         ctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGc  >  2:53213/1‑139 (MQ=255)                                                                                                                     |                                                                                                                               GCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGC  >  NC_000913/115545‑115787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑