Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,961	A→G	A632A (GCA→GCG)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,961	0	A	G	100.0%	179.4 / NA	51	A632A (GCA→GCG)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

TTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGA  >  NC_000913/1252827‑1253095                                                                                                                                       |                                                                                                                                       tttGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGc                                                                                                                                    >  2:459994/1‑139 (MQ=255) tttGCGGAAGTTGAAAAAGAGGCAATTACTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGc                                                                                                                                    <  2:820638/139‑1 (MQ=255)   tGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAg                                                                                                                                  >  1:102437/1‑139 (MQ=255)       gAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTa                                                                                                                              <  2:1181526/139‑1 (MQ=255)                                    aGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGAtt                                                                                                 >  2:442245/1‑139 (MQ=255)                                     gCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGAttt                                                                                                >  1:1007625/1‑139 (MQ=255)                                      ccccGGTCACAGGCGGTCGCATTAAGGAAATGTCGGCTTTCCTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               >  1:1303669/1‑139 (MQ=255)                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               >  1:94893/1‑139 (MQ=255)                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               >  2:113139/1‑139 (MQ=255)                                        cAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTg                                                                                             <  2:1109286/139‑1 (MQ=255)                                        cAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTg                                                                                             <  2:1007625/139‑1 (MQ=255)                                         aGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGa                                                                                            <  2:94893/139‑1 (MQ=255)                                           gTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          <  1:459994/139‑1 (MQ=255)                                                aGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGcc                                                                                                                        >  1:262838/1‑104 (MQ=255)                                                aGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGcc                                                                                                                        <  2:262838/104‑1 (MQ=255)                                                  gCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   <  1:442245/139‑1 (MQ=255)                                                    ggTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCGGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGt                                                                                 <  2:1303669/139‑1 (MQ=255)                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          >  2:1349089/1‑129 (MQ=255)                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          <  1:1349089/129‑1 (MQ=255)                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                >  2:654429/1‑139 (MQ=255)                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                <  2:102437/139‑1 (MQ=255)                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                <  1:113139/139‑1 (MQ=255)                                                        gCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATc                                                                             <  1:654429/139‑1 (MQ=255)                                                                      tCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGt                                                                                                                    <  2:595801/86‑1 (MQ=255)                                                                      tCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGt                                                                                                                    >  1:595801/1‑86 (MQ=255)                                                                              aCTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATc                                                                                      >  1:841727/1‑108 (MQ=255)                                                                              aCTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATc                                                                                      <  2:841727/108‑1 (MQ=255)                                                                                   ggATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGaaa                                                                                                      >  1:1343893/1‑87 (MQ=255)                                                                                   ggATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGaaa                                                                                                      <  2:1343893/87‑1 (MQ=255)                                                                                   ggATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACa                                                  >  1:1261037/1‑139 (MQ=255)                                                                                   ggATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACa                                                  <  2:1261037/139‑1 (MQ=255)                                                                                    gATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   >  1:622150/1‑105 (MQ=255)                                                                                    gATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   <  2:622150/105‑1 (MQ=255)                                                                                        ggCCGCACTACGCTGTGGCGG‑AAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTc                                            >  1:1309005/1‑139 (MQ=255)                                                                                         gCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   <  2:22330/100‑1 (MQ=255)                                                                                         gCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   >  1:22330/1‑100 (MQ=255)                                                                                             cACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGTAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        >  1:381786/1‑139 (MQ=255)                                                                                             cACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGTAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        <  2:381786/139‑1 (MQ=255)                                                                                                       tgGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATg                                                                                                                 >  1:541204/1‑56 (MQ=255)                                                                                                       tgGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATg                                                                                                                 <  2:541204/56‑1 (MQ=255)                                                                                                          cGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCt                           >  2:44090/1‑139 (MQ=255)                                                                                                                 tGAAGCAACATAGCATTGATGCGGGGCAGTTTAAGCGcc                                                                                                                        <  1:522233/39‑1 (MQ=255)                                                                                                                 tGAAGCAACATAGCATTGATGCGGGGCAGTTTAAGCGcc                                                                                                                        >  2:522233/1‑39 (MQ=255)                                                                                                                         cATGGCATTGATGCGGGGCATTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        <  1:977346/111‑1 (MQ=255)                                                                                                                         cATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        >  2:977346/1‑111 (MQ=255)                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACa                                                  <  2:934836/91‑1 (MQ=255)                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACa                                                  >  1:934836/1‑91 (MQ=255)                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCg                <  2:937180/125‑1 (MQ=255)                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCg                >  1:937180/1‑125 (MQ=255)                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGa  >  2:142158/1‑139 (MQ=255)                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGa  >  1:628264/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGA  >  NC_000913/1252827‑1253095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑