Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,528	A→G	I92I (ATT→ATC)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,528	0	A	G	100.0%	186.4 / NA	52	I92I (ATT→ATC)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

CGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCA  >  NC_000913/1260401‑1260655                                                                                                                                |                                                                                                                                cGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACaaa                                                                                                                      >  2:363787/1‑139 (MQ=255)                     aTCAGGCGACCAAAGCGTGCCAGACCCGTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaaa                                                                                                  >  1:816785/1‑139 (MQ=255)                      tCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAAc                                                                                                 >  2:73021/1‑139 (MQ=255)                         ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                              >  1:692889/1‑139 (MQ=255)                          gCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa                                                                                             <  2:113491/139‑1 (MQ=255)                               cAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg                                                                                        <  2:154639/139‑1 (MQ=255)                               cAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg                                                                                        <  1:731965/139‑1 (MQ=255)                                   gCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGTTTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGac                                                                                    <  1:971227/139‑1 (MQ=255)                                      tGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                                 <  1:578261/139‑1 (MQ=255)                                                aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       >  1:71427/1‑139 (MQ=255)                                                aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  2:39663/139‑1 (MQ=255)                                                 tCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       >  2:744368/1‑138 (MQ=255)                                                 tCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  1:744368/138‑1 (MQ=255)                                                  cAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCa                                                                     <  2:1191168/139‑1 (MQ=255)                                                     aaTCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATg                                                                  >  1:443873/1‑139 (MQ=255)                                                               aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                                               >  2:72214/1‑132 (MQ=255)                                                               aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                                               <  1:72214/132‑1 (MQ=255)                                                                tCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGc                                                       <  1:827277/139‑1 (MQ=255)                                                                 ccccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCa                                                      >  2:991717/1‑139 (MQ=255)                                                                   ccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAAc                                                    >  1:1068033/1‑139 (MQ=255)                                                                     gACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAg                                                  >  2:542771/1‑139 (MQ=255)                                                                     gACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGAACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAg                                                  <  1:810693/139‑1 (MQ=255)                                                                         gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCg                                                                             >  2:266366/1‑108 (MQ=255)                                                                         gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCg                                                                             <  1:266366/108‑1 (MQ=255)                                                                         gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  2:226712/114‑1 (MQ=255)                                                                         gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       >  1:226712/1‑114 (MQ=255)                                                                         gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACCGTTGCGACACGGGATAGAGGACTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                                              <  1:1168944/139‑1 (MQ=255)                                                                          cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGa                                                                                                         >  2:177380/1‑79 (MQ=255)                                                                          cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGa                                                                                                         <  1:177380/79‑1 (MQ=255)                                                                              gTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATACAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGtat                                         <  2:816785/139‑1 (MQ=255)                                                                                 gCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGtatata                                      <  2:71427/139‑1 (MQ=255)                                                                                   aaCCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaaa                                    <  1:542771/139‑1 (MQ=255)                                                                                   aaCCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaaa                                    <  2:692889/139‑1 (MQ=255)                                                                                    aCCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACcaca                                                                                                                        >  1:294368/1‑54 (MQ=255)                                                                                    aCCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACcaca                                                                                                                        <  2:294368/54‑1 (MQ=255)                                                                                         cagTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTAc                              >  2:1112733/1‑139 (MQ=255)                                                                                             ccTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       >  2:1249797/1‑94 (MQ=255)                                                                                             ccTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  1:1249797/94‑1 (MQ=255)                                                                                              cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCACCGCAACAGCTGCGGTATATAAACCGTACTgggg                         >  1:837275/1‑139 (MQ=255)                                                                                                 ccAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGc                      <  1:363787/139‑1 (MQ=255)                                                                                                    gTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                            >  2:758497/1‑130 (MQ=255)                                                                                                    gTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                            <  1:758497/130‑1 (MQ=255)                                                                                                     tCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca                  <  1:991717/139‑1 (MQ=255)                                                                                                     tCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca                  <  2:443873/139‑1 (MQ=255)                                                                                                            tGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTAc           <  2:837275/139‑1 (MQ=255)                                                                                                              ttGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa                                                                                                   <  2:151361/49‑1 (MQ=255)                                                                                                              ttGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa                                                                                                   >  1:151361/1‑49 (MQ=255)                                                                                                               tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa        >  1:404087/1‑139 (MQ=255)                                                                                                               tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa        <  1:349659/139‑1 (MQ=255)                                                                                                                 cGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTg                                                                                      <  1:1352428/59‑1 (MQ=255)                                                                                                                 cGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTg                                                                                      >  2:1352428/1‑59 (MQ=255)                                                                                                                     acGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCa  <  1:1030676/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                CGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCA  >  NC_000913/1260401‑1260655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑