Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,275,815 T→C E5E (GAA→GAG)  narL ← response regulator in two‑component regulatory system with NarX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,275,8150TC100.0% 193.3 / NA 55E5E (GAA→GAG) narLresponse regulator in two‑component regulatory system with NarX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/31);  total (24/31)

CTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCG  >  NC_000913/1275683‑1275948
                                                                                                                                    |                                                                                                                                     
cTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGa                                                                                                                                 <  2:1322166/139‑1 (MQ=255)
   cGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTa                                                                                                                              <  2:1129087/139‑1 (MQ=255)
         ttCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATg                                                                                                                        >  2:199530/1‑139 (MQ=255)
            aaTACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                     <  1:993022/139‑1 (MQ=255)
             aTACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTa                                                                                                                    <  1:199530/139‑1 (MQ=255)
              tACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   <  2:105943/139‑1 (MQ=255)
                cccTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATAGCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTGCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAtct                                                                                                                 <  1:105761/139‑1 (MQ=255)
                    gTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCtt                                                                                                             <  2:287707/139‑1 (MQ=255)
                               cTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                     >  2:101053/1‑120 (MQ=255)
                               cTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                     <  1:101053/120‑1 (MQ=255)
                                    ttCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGt                                                                                             >  1:433253/1‑139 (MQ=255)
                                    ttCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGt                                                                                             >  2:962349/1‑139 (MQ=255)
                                          aaCCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                       <  1:415152/139‑1 (MQ=255)
                                              aCGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTc                                                                                                                                     >  1:1089124/1‑89 (MQ=255)
                                              aCGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTc                                                                                                                                     <  2:1089124/89‑1 (MQ=255)
                                                       tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTg                                                                          >  2:384757/1‑139 (MQ=255)
                                                       tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTg                                                                          <  1:1149727/139‑1 (MQ=255)
                                                                 tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   <  2:663093/88‑1 (MQ=255)
                                                                 tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   >  1:663093/1‑88 (MQ=255)
                                                                 tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACtt                                                                >  2:991899/1‑139 (MQ=255)
                                                                 tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACtt                                                                <  2:1258428/139‑1 (MQ=255)
                                                                      aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                <  2:527500/70‑1 (MQ=255)
                                                                      aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                >  1:527500/1‑70 (MQ=255)
                                                                      aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTAc                                                                                                                             >  1:203463/1‑73 (MQ=255)
                                                                      aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTAc                                                                                                                             <  2:203463/73‑1 (MQ=255)
                                                                      aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTg                                                           >  2:609304/1‑139 (MQ=255)
                                                                           cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   >  1:1267106/1‑78 (MQ=255)
                                                                           cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   <  2:1267106/78‑1 (MQ=255)
                                                                           cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGcc                                                         <  1:1244448/136‑1 (MQ=255)
                                                                           cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGcc                                                         >  2:1244448/1‑136 (MQ=255)
                                                                           cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCAcc                                                      >  1:663464/1‑139 (MQ=255)
                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCg                                                              >  2:1130978/1‑128 (MQ=255)
                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCg                                                              <  1:1130978/128‑1 (MQ=255)
                                                                                ttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGAtt                                                 >  2:709856/1‑139 (MQ=255)
                                                                                     ccAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                           <  1:234339/108‑1 (MQ=255)
                                                                                     ccAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                           >  2:234339/1‑108 (MQ=255)
                                                                                          tCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGAcggcg                                       <  1:709856/139‑1 (MQ=255)
                                                                                                   cGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTc                                                                                                                           >  1:651829/1‑46 (MQ=255)
                                                                                                   cGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTc                                                                                                                           <  2:651829/46‑1 (MQ=255)
                                                                                                      gTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                           <  1:962349/139‑1 (MQ=255)
                                                                                                       tGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCg                          <  1:991899/139‑1 (MQ=255)
                                                                                                               aaTCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   <  2:510956/42‑1 (MQ=255)
                                                                                                               aaTCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                   >  1:510956/1‑42 (MQ=255)
                                                                                                               aaTCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATaa                                                                               >  2:587553/1‑78 (MQ=255)
                                                                                                               aaTCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATaa                                                                               <  1:587553/78‑1 (MQ=255)
                                                                                                                  cagcagGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACt               <  2:663464/139‑1 (MQ=255)
                                                                                                                            gtagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCAc                                                       <  1:942957/89‑1 (MQ=255)
                                                                                                                            gtagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCAc                                                       >  2:942957/1‑89 (MQ=255)
                                                                                                                            gtagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGa     <  1:951744/139‑1 (MQ=255)
                                                                                                                             tagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                           <  1:58552/68‑1 (MQ=255)
                                                                                                                             tagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                           >  2:58552/1‑68 (MQ=255)
                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGAcgg                                         <  2:513896/100‑1 (MQ=255)
                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGAcgg                                         >  1:513896/1‑100 (MQ=255)
                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcg  >  1:223540/1‑139 (MQ=255)
                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcg  <  2:1052884/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCG  >  NC_000913/1275683‑1275948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑