Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,354,505 C→T S5N (AGC→AAC) 
*322* (TAG→TAA
sapC ←
sapB ←
antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,354,5050CT100.0% 113.8 / NA 36S5N (AGC→AAC) 
*322* (TAG→TAA
sapC
sapB
antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

AAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATC  >  NC_000913/1354372‑1354633
                                                                                                                                     |                                                                                                                                
aaaCCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                             >  1:764789/1‑139 (MQ=255)
 aaCCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGt                                                                                                                            <  1:560532/139‑1 (MQ=255)
  aCCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTa                                                                                                                           <  2:764789/139‑1 (MQ=255)
                      aGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGt                                                                                                       >  1:13244/1‑139 (MQ=255)
                       gTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAgg                                                                                                                        >  1:1024909/1‑121 (MQ=255)
                       gTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAgg                                                                                                                        <  2:1024909/121‑1 (MQ=255)
                        tACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGttt                                                                                                     >  2:827477/1‑139 (MQ=255)
                        tACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGttt                                                                                                     >  2:1208430/1‑139 (MQ=255)
                                gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                                   >  1:378973/1‑133 (MQ=255)
                                gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                                   <  2:378973/133‑1 (MQ=255)
                                gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGt                                                                                             >  1:1309048/1‑139 (MQ=255)
                                  cccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTa                                                                                           >  2:1025253/1‑139 (MQ=255)
                                      cgcAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                             <  2:581616/101‑1 (MQ=255)
                                      cgcAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                             >  1:581616/1‑101 (MQ=255)
                                       gcAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCAt                                                                                      <  2:183100/139‑1 (MQ=255)
                                                     cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCa                                                                                                                      <  1:463472/93‑1 (MQ=255)
                                                     cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCa                                                                                                                      >  2:463472/1‑93 (MQ=255)
                                                                    cGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTaa                                                         >  1:1036632/1‑139 (MQ=255)
                                                                    cGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTaa                                                         <  2:1309048/139‑1 (MQ=255)
                                                                           ataAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaataat                                                  <  2:1287480/139‑1 (MQ=255)
                                                                                   tGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTg                                          <  1:1025253/139‑1 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGt                                                                                                                            <  1:710211/56‑1 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGt                                                                                                                            >  2:710211/1‑56 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAc                                                                                                                   <  1:631472/65‑1 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAc                                                                                                                   >  2:631472/1‑65 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                             <  2:166823/71‑1 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                             >  1:166823/1‑71 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCagag                                                                                                <  1:1357034/84‑1 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCagag                                                                                                >  2:1357034/1‑84 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTaa                                                         >  2:260295/1‑123 (MQ=255)
                                                                                    gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTaa                                                         <  1:260295/123‑1 (MQ=255)
                                                                                                         gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcggc                    >  2:4324/1‑139 (MQ=255)
                                                                                                                   cgcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCt          <  1:4324/139‑1 (MQ=255)
                                                                                                                    gcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg            <  1:1133766/136‑1 (MQ=255)
                                                                                                                    gcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg            >  2:1133766/1‑136 (MQ=255)
                                                                                                                           gacagataCGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAACATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATc  <  1:158466/134‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
AAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATC  >  NC_000913/1354372‑1354633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: