Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,095	G→A	A126T (GCA→ACA)	tyrR →	aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,095	0	G	A	100.0%	83.8 / NA	28	A126T (GCA→ACA)	tyrR	aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/17);  total (11/17)

TGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCGCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGA  >  NC_000913/1386985‑1387225                                                                                                               |                                                                                                                                   tGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCacac                                                                                                                                 >  2:733488/1‑114 (MQ=255) tGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCacac                                                                                                                                 <  1:733488/114‑1 (MQ=255)  gCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTa                                                                                                       >  2:673828/1‑139 (MQ=255)   ccTGAACCTGTGGTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTAc                                                                                                      <  1:892011/139‑1 (MQ=255)    cTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACg                                                                                                     <  1:563496/139‑1 (MQ=255)        aCCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTgg                                                                                                 <  1:182569/139‑1 (MQ=255)             tgCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                    <  2:676793/131‑1 (MQ=255)             tgCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                    >  1:676793/1‑131 (MQ=255)             tgCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGa                                                                                            >  1:1292201/1‑139 (MQ=255)                  tctGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCg                                                                                       <  1:673828/139‑1 (MQ=255)                          tatGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCaa                                                                               >  1:536140/1‑139 (MQ=255)                                aaGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCg                                                                         >  1:327609/1‑139 (MQ=255)                                        tGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                    >  1:487814/1‑104 (MQ=255)                                        tGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                    <  2:487814/104‑1 (MQ=255)                                            tatGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCAt                                                             <  2:1292201/139‑1 (MQ=255)                                             atGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATg                                                            <  2:327609/139‑1 (MQ=255)                                                 cGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGt                                                        >  1:1327476/1‑139 (MQ=255)                                                  gAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                    >  1:246126/1‑94 (MQ=255)                                                  gAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                    <  2:246126/94‑1 (MQ=255)                                                                gTCAGCTTTTTGGGCAAAAATTGGCTCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGaa                                         >  1:928190/1‑139 (MQ=255)                                                                    gCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTc                                     <  2:21470/139‑1 (MQ=255)                                                                         ttGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGC‑TTAATTTTTTACGTTGACTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATg                               <  1:1215369/139‑1 (MQ=255)                                                                          tGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATg                               <  2:536140/139‑1 (MQ=255)                                                                                          cGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGttt               <  1:168918/139‑1 (MQ=255)                                                                                            ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTc                                     >  2:1355832/1‑115 (MQ=255)                                                                                            ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTc                                     <  1:1355832/115‑1 (MQ=255)                                                                                            ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCCGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGttt               <  1:990293/137‑1 (MQ=255)                                                                                            ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCCGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGttt               >  2:990293/1‑137 (MQ=255)                                                                                               gcgcAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCtt          <  2:1327476/139‑1 (MQ=255)                                                                                                       aTACCGCCACACAATTAATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACTCCTGTTTATCTTCAGGATGa  <  2:928190/139‑1 (MQ=255)                                                                                                               |                                                                                                                                   TGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCGCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGA  >  NC_000913/1386985‑1387225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑