Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,466,996	T→C	pseudogene (1605/2513 nt)	ydbA →	pseudogene, autotransporter homolog, interrupted by IS2 and IS30

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,996	0	T	C	100.0%	177.5 / NA	50	pseudogene (1605/2513 nt)	ydbA	pseudogene, autotransporter homolog, interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

GCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACT  >  NC_000913/1466871‑1467131                                                                                                                              |                                                                                                                                        gCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                            <  2:970672/139‑1 (MQ=255)  ccATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCggg                                                                                                                           <  1:1251108/139‑1 (MQ=255)                  aGGCGAGAGCACTATCACCAATGGTGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTa                                                                                                           >  2:478977/1‑139 (MQ=255)                  aGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTa                                                                                                           >  2:503720/1‑139 (MQ=255)                         agCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTgg                                                                                                    >  2:104991/1‑139 (MQ=255)                                 cACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTaac                                                                                            <  1:820705/139‑3 (MQ=255)                                      aTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg                                                                                                                  <  1:607610/112‑1 (MQ=255)                                      aTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg                                                                                                                  >  2:607610/1‑112 (MQ=255)                                       tGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAatat                                                                                                                     <  1:719051/108‑1 (MQ=255)                                       tGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAatat                                                                                                                     >  2:719051/1‑108 (MQ=255)                                        ggcggcACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTg                                                                                     >  1:690412/1‑139 (MQ=255)                                          cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt                                                                                   >  2:483209/1‑139 (MQ=255)                                          cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt                                                                                   >  2:341570/1‑139 (MQ=255)                                                        aGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     <  1:341570/139‑1 (MQ=255)                                                          aTCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     >  2:534132/1‑137 (MQ=255)                                                          aTCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     <  1:534132/137‑1 (MQ=255)                                                               cGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGtt                                                              <  1:478977/139‑1 (MQ=255)                                                               cGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGtt                                                              <  1:503720/139‑1 (MQ=255)                                                                  tAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTgag                                                           <  2:49008/139‑1 (MQ=255)                                                                         gCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTg                                                    >  1:408094/1‑139 (MQ=255)                                                                            aCCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTg                                                 >  2:467552/1‑139 (MQ=255)                                                                              cgcgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCgg                                                                           >  2:1017731/1‑111 (MQ=255)                                                                              cgcgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCgg                                                                           <  1:1017731/111‑1 (MQ=255)                                                                                cgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCt                                                                                          <  1:366634/94‑1 (MQ=255)                                                                                cgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCt                                                                                          >  2:366634/1‑94 (MQ=255)                                                                                cgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACaa                                             >  2:988203/1‑139 (MQ=255)                                                                                          tGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATcgcg                                                                                                                             <  1:292244/49‑1 (MQ=255)                                                                                          tGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATcgcg                                                                                                                             >  2:292244/1‑49 (MQ=255)                                                                                            gAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATcg                                                                                                                               <  1:25418/45‑1 (MQ=255)                                                                                            gAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATcg                                                                                                                               >  2:25418/1‑45 (MQ=255)                                                                                            gAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGtt                                                              <  2:99200/110‑1 (MQ=255)                                                                                            gAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGtt                                                              >  1:99200/1‑110 (MQ=255)                                                                                                 aCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAacaaca                            >  1:399533/1‑139 (MQ=255)                                                                                                  ccACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAAcaacaa                           <  2:690412/139‑1 (MQ=255)                                                                                                               aaaaGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATAttgtt              <  2:155384/139‑1 (MQ=255)                                                                                                               aaaaGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATAttgtt              <  1:104991/139‑1 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCAt                                                                                                               <  2:1113281/39‑1 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCAt                                                                                                               >  1:1113281/1‑39 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     <  1:1001395/81‑1 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     >  2:1001395/1‑81 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACg                                           <  1:792869/107‑1 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACg                                           >  2:792869/1‑107 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTgttgtt           >  2:852124/1‑139 (MQ=255)                                                                                                                  aGATTCCACGGACACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     >  2:934875/1‑81 (MQ=255)                                                                                                                  aGATTCCACGGACACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     <  1:934875/81‑1 (MQ=255)                                                                                                                    aTTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTc         <  1:467552/139‑1 (MQ=255)                                                                                                                      tCCACGGGCACCAAAATCGCAGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACa                                                       >  1:492297/1‑91 (MQ=255)                                                                                                                      tCCACGGGCACCAAAATCGCAGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACa                                                       <  2:492297/91‑1 (MQ=255)                                                                                                                       ccACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGa      >  1:424882/1‑139 (MQ=255)                                                                                                                           gggCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACt  <  1:852124/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                        GCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACT  >  NC_000913/1466871‑1467131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑