Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,513,468 T→C S218P (TCC→CCC)  ydcT → putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,513,4680TC100.0% 85.5 / NA 26S218P (TCC→CCC) ydcTputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

CTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAC  >  NC_000913/1513339‑1513594
                                                                                                                                 |                                                                                                                              
cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                       >  1:216676/1‑139 (MQ=255)
cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                       <  2:1093304/139‑1 (MQ=255)
    aaCTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                                   >  2:377565/1‑139 (MQ=255)
          aaaaaCTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTatat                                                                                                             >  2:944407/1‑139 (MQ=255)
                                      ttttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                         <  1:979494/99‑1 (MQ=255)
                                      ttttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                         >  2:979494/1‑99 (MQ=255)
                                        ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                       >  1:1007375/1‑99 (MQ=255)
                                        ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                       <  2:1007375/99‑1 (MQ=255)
                                        ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                               <  1:377565/139‑1 (MQ=255)
                                                         gATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATg                                                              >  2:1033424/1‑139 (MQ=255)
                                                              gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATcccc                                                                                                                             >  2:717132/1‑71 (MQ=255)
                                                              gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATcccc                                                                                                                             <  1:717132/71‑1 (MQ=255)
                                                                   aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                         <  2:349605/70‑1 (MQ=255)
                                                                   aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                         >  1:349605/1‑70 (MQ=255)
                                                                    aGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                     >  2:723546/1‑73 (MQ=255)
                                                                    aGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                     <  1:723546/73‑1 (MQ=255)
                                                                      cGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACt                                                 >  1:723804/1‑139 (MQ=255)
                                                                         tATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGAt                                              <  1:1033424/139‑1 (MQ=255)
                                                                                         tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                     >  1:782046/1‑52 (MQ=255)
                                                                                         tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                     <  2:782046/52‑1 (MQ=255)
                                                                                         tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                 <  2:627256/88‑1 (MQ=255)
                                                                                         tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                 >  1:627256/1‑88 (MQ=255)
                                                                                                 ttttCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGAc                      >  1:605338/1‑139 (MQ=255)
                                                                                                                gCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCt       >  1:299400/1‑139 (MQ=255)
                                                                                                                 cATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTg      <  2:216676/139‑1 (MQ=255)
                                                                                                                     gAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAc  <  1:463212/139‑1 (MQ=255)
                                                                                                                           gTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAc  >  2:1365834/1‑133 (MQ=255)
                                                                                                                           gTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAc  <  1:1365834/133‑1 (MQ=255)
                                                                                                                                 |                                                                                                                              
CTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAC  >  NC_000913/1513339‑1513594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: