Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,360	C→T	D320D (GAC→GAT)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,360	0	C	T	100.0%	111.2 / NA	35	D320D (GAC→GAT)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/18);  total (17/18)

TACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCT  >  NC_000913/1548234‑1548475                                                                                                                               |                                                                                                                    tACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGc                                                                                                         >  2:1142382/1‑139 (MQ=255)                 cTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                         <  1:728547/139‑1 (MQ=255)                    tCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                      <  1:1042511/139‑1 (MQ=255)                    tCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                      >  2:74129/1‑139 (MQ=255)                                   tACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                       <  2:268205/139‑1 (MQ=255)                                               aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                  >  1:323825/1‑116 (MQ=255)                                               aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                  <  2:323825/116‑1 (MQ=255)                                                             cGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaa                                             >  1:1220013/1‑139 (MQ=255)                                                              gCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaa                                            >  1:392017/1‑139 (MQ=255)                                                               ccGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaaa                                           <  1:299104/139‑1 (MQ=255)                                                                          atgcttaacacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGa                                >  2:841340/8‑139 (MQ=255)                                                                            gCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGaaa                              >  1:714896/1‑139 (MQ=255)                                                                                 acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCaa                                                                                 >  2:1127471/1‑83 (MQ=255)                                                                                 acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCaa                                                                                 <  1:1127471/83‑1 (MQ=255)                                                                                 acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                   <  1:1194143/97‑1 (MQ=255)                                                                                 acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                   >  2:1194143/1‑97 (MQ=255)                                                                                            aGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGa              <  2:1220013/139‑1 (MQ=255)                                                                                             gCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                      >  2:979349/1‑66 (MQ=255)                                                                                             gCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                      <  1:979349/66‑1 (MQ=255)                                                                                               ctgctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaa                                             <  2:430268/105‑1 (MQ=255)                                                                                               ctgctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaa                                             >  1:430268/1‑105 (MQ=255)                                                                                                 gctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgacg                                                                                               <  1:567496/53‑1 (MQ=255)                                                                                                 gctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgacg                                                                                               >  2:567496/1‑53 (MQ=255)                                                                                                  ctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGa                                                              >  1:735866/1‑85 (MQ=255)                                                                                                  ctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGa                                                              <  2:735866/85‑1 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg    <  1:841340/139‑1 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg    <  1:74129/139‑1 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg    <  2:714896/139‑1 (MQ=255)                                                                                                      tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg    <  2:787693/139‑1 (MQ=255)                                                                                                       gCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgac                                                                                                <  1:566944/46‑1 (MQ=255)                                                                                                       gCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgac                                                                                                >  2:566944/1‑46 (MQ=255)                                                                                                        cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCCGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCATCTCGATGAACACGGCTATGCGCAACGCCATGAAACACAGACTCATCCGCGCt  >  1:1353306/1‑139 (MQ=255)                                                                                                        cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt  >  1:548270/1‑139 (MQ=255)                                                                                                        cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt  <  2:548270/139‑1 (MQ=255)                                                                                                        cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt  >  2:297362/1‑139 (MQ=255)                                                                                                                               |                                                                                                                    TACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCT  >  NC_000913/1548234‑1548475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑