Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	167,743	C→T	T87I (ACC→ATC)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	167,743	0	C	T	100.0%	151.5 / NA	47	T87I (ACC→ATC)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

ACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAG  >  NC_000913/167610‑167877                                                                                                                                      |                                                                                                                                       aCCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGAtcgccg                                                                                                                                   <  1:666110/139‑1 (MQ=255)     ttACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAaga                                                                                                                               >  2:586901/1‑139 (MQ=255)     ttACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAaga                                                                                                                               >  1:489960/1‑139 (MQ=255)       aCCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                             >  1:784092/1‑139 (MQ=255)       aCCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                             <  2:784092/139‑1 (MQ=255)       aCCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                             <  1:644573/139‑1 (MQ=255)                                      gggggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaaga                                                                                              <  1:464445/139‑1 (MQ=255)                                            ctgctgCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                        <  2:489960/139‑1 (MQ=255)                                                  cAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTa                                                                                  <  2:1141676/139‑1 (MQ=255)                                                         tGCGGCGCGATAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                            <  2:1329251/138‑1 (MQ=255)                                                         tGCGGCGCGATAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                            >  1:1329251/1‑138 (MQ=255)                                                         tGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGa                                                                                                                                  >  2:466129/1‑84 (MQ=255)                                                         tGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGa                                                                                                                                  <  1:466129/84‑1 (MQ=255)                                                          gcggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGGCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCg                                                                                                            >  2:1311641/1‑105 (MQ=255)                                                          gcggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGGCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCg                                                                                                            <  1:1311641/105‑1 (MQ=255)                                                            ggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        <  1:586901/139‑1 (MQ=255)                                                            ggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        <  1:898525/139‑1 (MQ=255)                                                                               ggCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTg                                                     >  2:637557/1‑139 (MQ=255)                                                                                gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      >  2:73563/1‑121 (MQ=255)                                                                                gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      <  1:73563/121‑1 (MQ=255)                                                                                gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTgg                                                    >  1:1007998/1‑139 (MQ=255)                                                                                gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTgg                                                    <  1:637557/139‑1 (MQ=255)                                                                                gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTgg                                                    >  1:527667/1‑139 (MQ=255)                                                                                  aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACg                                                                              <  1:6868/111‑1 (MQ=255)                                                                                  aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACg                                                                              >  2:6868/1‑111 (MQ=255)                                                                                    tAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCa                                                <  2:477656/139‑1 (MQ=255)                                                                                        aCCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            <  2:1007998/139‑1 (MQ=255)                                                                                            aTACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                        >  2:366757/1‑59 (MQ=255)                                                                                            aTACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                        <  1:366757/59‑1 (MQ=255)                                                                                                  cGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGt                                                                                                    >  2:764159/1‑73 (MQ=255)                                                                                                  cGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGt                                                                                                    <  1:764159/73‑1 (MQ=255)                                                                                                              tGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCAt                      >  1:1028842/1‑139 (MQ=255)                                                                                                                        tATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAacac                                        <  1:699609/111‑1 (MQ=255)                                                                                                                        tATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAacac                                        >  2:699609/1‑111 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                            <  2:85289/67‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                            >  1:85289/1‑67 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCg                                                                           >  2:25710/1‑68 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCg                                                                           <  1:25710/68‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        >  2:962199/1‑71 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        <  1:962199/71‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            <  2:421428/99‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            >  2:1328222/1‑99 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            >  1:421428/1‑99 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            <  1:1328222/99‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCgg        <  2:67056/135‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCgg        >  1:67056/1‑135 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGa    >  2:22626/1‑139 (MQ=255)                                                                                                                                  gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAg  >  2:61529/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       ACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAG  >  NC_000913/167610‑167877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑