Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,709,163	T→C	S8P (TCC→CCC)	rsxD →	SoxR iron‑sulfur cluster reduction factor component, putative membrane protein of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,709,163	0	T	C	100.0%	51.9 / NA	16	S8P (TCC→CCC)	rsxD	SoxR iron‑sulfur cluster reduction factor component, putative membrane protein of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/9);  total (7/9)

AGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGTTA  >  NC_000913/1709042‑1709298                                                                                                                           |                                                                                                                                        agaagaACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc                                                                                                                        <  2:676592/139‑1 (MQ=255)              gATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta                                                                                                                                  >  1:381335/1‑116 (MQ=255)              gATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta                                                                                                                                  <  2:381335/116‑1 (MQ=255)              gATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACAt                                                                                                           <  2:61289/139‑1 (MQ=255)                    cgcAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCgcgc                                                                                                      >  1:239440/1‑139 (MQ=255)                    cgcAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa                                                                                                     <  2:1274601/139‑1 (MQ=255)                         aGCGGCAGTTGCCGCGGCTCTTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCATCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc                                                                                                                        <  1:889133/115‑1 (MQ=255)                         aGCGGCAGATGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc                                                                                                                        >  2:889133/1‑115 (MQ=255)                                                                 aaaaGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcg                                                         >  1:397937/1‑139 (MQ=255)                                                                                        tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc                                                                                                                        >  1:895203/1‑53 (MQ=255)                                                                                        tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc                                                                                                                        <  2:895203/53‑1 (MQ=255)                                                                                        tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg                                  <  2:397937/139‑1 (MQ=255)                                                                                            cGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCATCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTAct                              <  1:152409/139‑1 (MQ=255)                                                                                            cGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTAct                              <  2:239440/139‑1 (MQ=255)                                                                                               ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACtctc                           >  2:980849/1‑139 (MQ=255)                                                                                                     aaTGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAg                     >  2:966118/1‑139 (MQ=255)                                                                                                                      ctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCTCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGt    <  1:980849/139‑1 (MQ=255)                                                                                                                        agccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGTTa  >  2:981829/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                        AGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCC‑AAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCGGTTA  >  NC_000913/1709042‑1709298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑