Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,006	G→A	A437T (GCT→ACT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,006	0	G	A	100.0%	183.3 / NA	58	A437T (GCT→ACT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/35);  total (23/35)

CCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAAT  >  NC_000913/1756878‑1757130                                                                                                                                 |                                                                                                                             ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                    <  2:1245073/139‑1 (MQ=255) ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                    <  2:234543/139‑1 (MQ=255)   accaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCg                                                                                                                  <  1:131520/139‑1 (MQ=255)     caTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGt                                                                                                                <  1:452769/139‑1 (MQ=255)                tGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACaa                                                                                                     <  2:125753/139‑1 (MQ=255)                  accaccAACGAAAAAACGGCTCATCAGTTGGTTCTGAGCAAATGCGGTGTGCGGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                   >  1:140310/1‑139 (MQ=255)                  accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                        <  1:189722/134‑1 (MQ=255)                  accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                        >  2:189722/1‑134 (MQ=255)                  accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                   >  1:388551/1‑139 (MQ=255)                  accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                   >  2:389630/1‑139 (MQ=255)                     accaACGAAAAAACGGCTCATCAGTTGGTACTGAGCGAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTg                                                                                                <  2:405802/139‑1 (MQ=255)                          cGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGtt                                                                                           <  1:389630/139‑1 (MQ=255)                           gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                                                                                          <  2:727478/139‑1 (MQ=255)                            aaaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGt                                                                                         <  1:264707/139‑1 (MQ=255)                                                     gAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTa                                                                >  2:674956/1‑139 (MQ=255)                                                           aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGtt                                                                                           >  1:908104/1‑106 (MQ=255)                                                           aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGtt                                                                                           <  2:908104/106‑1 (MQ=255)                                                           aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGc                                                          >  2:28710/1‑139 (MQ=255)                                                                ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                          <  1:529567/118‑1 (MQ=255)                                                                ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                          >  2:529567/1‑118 (MQ=255)                                                                ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCac                                                     >  2:661364/1‑139 (MQ=255)                                                                    gCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                 <  1:504745/139‑1 (MQ=255)                                                                     ccGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCActacta                                                >  2:1362964/1‑139 (MQ=255)                                                                     ccGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCActacta                                                >  2:1038031/1‑139 (MQ=255)                                                                        cAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGt                                                                                                                <  2:1255303/72‑1 (MQ=255)                                                                        cAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGt                                                                                                                >  1:1255303/1‑72 (MQ=255)                                                                              gTTAAAGAGATCAATACTTCTGAAGATTTCTACCGTCTGGGTAAATAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTGTGGTTTCTGGTGCACTGGTACCGAACGGCACTACTAACACCGCac                                       <  1:434150/139‑2 (MQ=255)                                                                                       aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTgg                                                                                                            >  1:212214/1‑61 (MQ=255)                                                                                       aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTgg                                                                                                            <  2:212214/61‑1 (MQ=255)                                                                                       aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                              <  1:1038031/139‑1 (MQ=255)                                                                                       aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                              <  1:674956/139‑1 (MQ=255)                                                                                       aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                              <  1:1362964/139‑1 (MQ=255)                                                                                         cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGCAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                       >  1:709601/1‑112 (MQ=255)                                                                                         cACCTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                       <  2:709601/112‑1 (MQ=255)                                                                                          aCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                            <  1:343957/138‑1 (MQ=255)                                                                                          aCTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                            >  2:343957/1‑138 (MQ=255)                                                                                                 cTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAata                    <  2:245994/139‑1 (MQ=255)                                                                                                     tgatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGc                >  2:931168/1‑139 (MQ=255)                                                                                                      gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                                                                                          <  1:59644/64‑1 (MQ=255)                                                                                                      gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                                                                                          >  2:59644/1‑64 (MQ=255)                                                                                                         ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                               <  1:626227/56‑1 (MQ=255)                                                                                                         ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                               >  2:626227/1‑56 (MQ=255)                                                                                                         ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCtttt            <  2:388551/139‑1 (MQ=255)                                                                                                           cTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTtgt          <  2:140310/139‑1 (MQ=255)                                                                                                              ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCa                                                      <  1:1312446/92‑1 (MQ=255)                                                                                                              ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCa                                                      >  2:1312446/1‑92 (MQ=255)                                                                                                                gTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                 >  2:1351795/1‑95 (MQ=255)                                                                                                                gTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                 <  1:1351795/95‑1 (MQ=255)                                                                                                                gTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaatt     <  1:627775/139‑1 (MQ=255)                                                                                                                 tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                       <  2:423927/88‑1 (MQ=255)                                                                                                                 tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                       >  1:423927/1‑88 (MQ=255)                                                                                                                 tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCa                                <  2:412866/111‑1 (MQ=255)                                                                                                                 tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCa                                >  1:412866/1‑111 (MQ=255)                                                                                                                 tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaatta    <  1:931168/139‑1 (MQ=255)                                                                                                                  cTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaa   <  2:440555/139‑1 (MQ=255)                                                                                                                   tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat  <  1:594394/139‑1 (MQ=255)                                                                                                                     ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaa       >  2:975737/1‑132 (MQ=255)                                                                                                                     ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaa       <  1:975737/132‑1 (MQ=255)                                                                                                                                 |                                                                                                                             CCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAAT  >  NC_000913/1756878‑1757130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑