Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,764,018 C→T *123* (TAG→TAA sufA ← Fe‑S cluster assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,0180CT100.0% 82.2 / NA 26*123* (TAG→TAAsufAFe‑S cluster assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/11);  total (15/11)

TGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCA  >  NC_000913/1763905‑1764145
                                                                                                                 |                                                                                                                               
tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATCGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagcc                                                                                                       >  2:726868/1‑139 (MQ=255)
  ccAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagccac                                                                                                     >  2:1054366/1‑139 (MQ=255)
        cATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                                            >  1:163277/1‑126 (MQ=255)
        cATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                                            <  2:163277/126‑1 (MQ=255)
           cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                            <  1:1054366/139‑1 (MQ=255)
                        ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTta                                                                                                                               >  1:47164/1‑91 (MQ=255)
                        ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTta                                                                                                                               <  2:47164/91‑1 (MQ=255)
                                   cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                    >  2:632116/1‑139 (MQ=255)
                                       tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                           <  1:336806/112‑1 (MQ=255)
                                       tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                           >  2:336806/1‑112 (MQ=255)
                                        ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                                                               <  1:726868/139‑1 (MQ=255)
                                        ttATAATTCACCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                                              >  1:1107949/1‑92 (MQ=255)
                                        ttATAATTCACCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                                              <  2:1107949/92‑1 (MQ=255)
                                                         gccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacg                                              >  2:879359/1‑139 (MQ=255)
                                                                              cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa                                                                                    <  2:782103/76‑1 (MQ=255)
                                                                              cgtcattGCTTCAGTATAACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTGTGGGCTTTa                                                                                    >  1:782103/6‑81 (MQ=255)
                                                                                         cAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAAc               >  1:500480/1‑139 (MQ=255)
                                                                                          aGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACg              >  1:1309414/1‑139 (MQ=255)
                                                                                            tATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc            <  2:1309414/139‑1 (MQ=255)
                                                                                                aCGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                            <  1:510878/55‑1 (MQ=255)
                                                                                                aCGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                            >  2:510878/1‑55 (MQ=255)
                                                                                                 cGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                   <  1:88761/47‑1 (MQ=255)
                                                                                                 cGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                   >  2:88761/1‑47 (MQ=255)
                                                                                                      cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                           <  1:543511/50‑1 (MQ=255)
                                                                                                      cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                           >  2:543511/1‑50 (MQ=255)
                                                                                                      cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCa  >  2:465973/1‑139 (MQ=255)
                                                                                                                 |                                                                                                                               
TGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCA  >  NC_000913/1763905‑1764145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: