Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,788,278	G→A	*278* (TAG→TAA)	ppsR →	PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,788,278	0	G	A	100.0%	110.7 / NA	35	*278* (TAG→TAA)	ppsR	PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

CAGGATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCC  >  NC_000913/1788143‑1788401                                                                                                                                        |                                                                                                                            cAGGATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTGGCCGAATGTACTAaaga                                                                                                                          <  2:854983/139‑1 (MQ=255)                             ttGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                              <  1:654596/139‑1 (MQ=255)                                     tAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTa                                                                                      >  1:415196/1‑139 (MQ=255)                                     tAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTa                                                                                      >  1:600308/1‑139 (MQ=255)                                                      ggATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTg                                                                                                 >  2:1326429/1‑111 (MQ=255)                                                      ggATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTg                                                                                                 <  1:1326429/111‑1 (MQ=255)                                                                 aCCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTa                                                                                                         >  2:43080/1‑92 (MQ=255)                                                                 aCCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTa                                                                                                         <  1:43080/92‑1 (MQ=255)                                                                  ccAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGa                                                                   <  2:136139/129‑1 (MQ=255)                                                                  ccAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGa                                                                   >  1:136139/1‑129 (MQ=255)                                                                     attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAAc                                                                                                                        <  1:1037234/73‑1 (MQ=255)                                                                     attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAAc                                                                                                                        >  2:1037234/1‑73 (MQ=255)                                                                         ttCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGaa                                                  <  2:415196/139‑1 (MQ=255)                                                                          tCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGaaa                                                 >  1:499012/1‑139 (MQ=255)                                                                                  agagATTGCCACCCAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTATTAAAGAACTAGTGCATTAGCTTACTTTTTTGTTATCATGGTAACCACCCGTCGAGGTGTGACACACCTCGCACTTGAAATCagcagc                                         <  2:869807/139‑1 (MQ=255)                                                                                    agaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGa                                       <  2:600308/139‑1 (MQ=255)                                                                                      aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAaccac                                                                                 <  1:832508/95‑1 (MQ=255)                                                                                      aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCg                                                                           >  2:1137363/1‑101 (MQ=255)                                                                                      aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCg                                                                           <  1:1137363/101‑1 (MQ=255)                                                                                      aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCCAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAaccac                                                                                 >  2:832508/1‑95 (MQ=255)                                                                                       ttGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGacaca                                                               <  2:1221057/112‑1 (MQ=255)                                                                                       ttGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGacaca                                                               >  1:1221057/1‑112 (MQ=255)                                                                                                aGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGc                                                                                                                  >  2:1305914/1‑52 (MQ=255)                                                                                                aGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGc                                                                                                                  <  1:1305914/52‑1 (MQ=255)                                                                                                aGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGt                           >  2:1106331/1‑139 (MQ=255)                                                                                                         atatCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCg                                                                           >  1:102477/1‑82 (MQ=255)                                                                                                         atatCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCg                                                                           <  2:102477/82‑1 (MQ=255)                                                                                                         atatCATGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCAt                  <  2:499012/139‑1 (MQ=255)                                                                                                               tGGGCCTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTc            >  1:1246814/1‑139 (MQ=255)                                                                                                                    cTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                            >  1:629810/1‑38 (MQ=255)                                                                                                                    cTTAGTCGCCGAATGTACTAAAGAACTAGTGCATTAGc                                                                                                            <  2:629810/38‑1 (MQ=255)                                                                                                                      tAGTCGCCGAATGTACTAAAGAACTAGTGCATTAACTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTg                          <  2:432386/118‑1 (MQ=255)                                                                                                                      tAGTCGCCGAATGTACTAAAGAACTAGTGCATTAACTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTg                          >  1:432386/1‑118 (MQ=255)                                                                                                                       aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                              <  2:587957/49‑1 (MQ=255)                                                                                                                       aGTCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTa                                                                                              >  1:587957/1‑49 (MQ=255)                                                                                                                         tCGCCGAATGTACTAAAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTcc  <  1:1106331/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                            CAGGATGGAAGTCGCGGAAGTGGAAGCCTTGTACCGTAAAAATCAGATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCC  >  NC_000913/1788143‑1788401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑