Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,841,897 G→A *136* (TAG→TAA
T80I (ACT→ATT) 
nudG →
ynjH ←
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,8970GA100.0% 167.8 / NA 53*136* (TAG→TAA
T80I (ACT→ATT) 
nudG
ynjH
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/26);  total (27/26)

ACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGC  >  NC_000913/1841774‑1842023
                                                                                                                           |                                                                                                                              
acaTGAACATCAGGCGCTGGTCTGGTGATCACCTGAAGAGGCGCTGCAATATCAGCTGCCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGt                                                                                                                 >  2:549275/1‑139 (MQ=255)
 caTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtc                                                                                                                >  1:88920/1‑139 (MQ=255)
  aTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTa                                                                                                                           <  2:344444/127‑1 (MQ=255)
  aTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTa                                                                                                                           >  1:344444/1‑127 (MQ=255)
                             cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgca                                                                                               <  2:460443/128‑1 (MQ=255)
                             cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgca                                                                                               >  1:460443/1‑128 (MQ=255)
                             cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCt                                                                                    >  1:280810/1‑139 (MQ=255)
                               ccTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATTCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTc                                                                                  <  1:965555/139‑1 (MQ=255)
                                         cgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAAtt                                                                        <  2:88920/139‑1 (MQ=255)
                                              cAATATCCGCTGGCCCCTTCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACGAGCGGATTAATGCTAAGGATTTTGTCATCATGCTGGCAGTGCAGCAGTATTTCTGCGGCTTTAATTACCGc                                                                   <  1:549275/139‑1 (MQ=255)
                                                      gCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCg                                                                                 >  2:1328060/1‑117 (MQ=255)
                                                      gCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCg                                                                                 <  1:1328060/117‑1 (MQ=255)
                                                        tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      <  2:1190833/94‑1 (MQ=255)
                                                        tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      >  1:1190833/1‑94 (MQ=255)
                                                        tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                         >  1:930715/1‑139 (MQ=255)
                                                                                taGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                 >  1:278089/1‑139 (MQ=255)
                                                                                  gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                              <  1:923532/60‑1 (MQ=255)
                                                                                  gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                              >  2:923532/1‑60 (MQ=255)
                                                                                    ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACt                             <  2:278089/139‑1 (MQ=255)
                                                                                      cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      >  2:1136954/1‑64 (MQ=255)
                                                                                      cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      <  1:1136954/64‑1 (MQ=255)
                                                                                       gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                          <  2:1345663/43‑1 (MQ=255)
                                                                                       gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                          >  1:1345663/1‑43 (MQ=255)
                                                                                       gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGc                                                                               <  2:703549/86‑1 (MQ=255)
                                                                                       gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGc                                                                               >  1:703549/1‑86 (MQ=255)
                                                                                       gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGc                                                                   <  1:833151/98‑1 (MQ=255)
                                                                                       gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCGAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGc                                                                   >  2:833151/1‑98 (MQ=255)
                                                                                               cTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGc                  >  1:47464/1‑139 (MQ=255)
                                                                                                 ttACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                 >  2:1230659/1‑58 (MQ=255)
                                                                                                 ttACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                 <  1:1230659/58‑1 (MQ=255)
                                                                                                 ttACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGTGTACAgtgttg                <  1:794067/139‑6 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      >  2:801358/1‑52 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      <  1:801358/52‑1 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      <  1:1304002/52‑1 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                      >  2:1304002/1‑52 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                   <  1:1069629/71‑1 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                   >  2:1069629/1‑71 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                              <  2:1227600/92‑1 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                              >  1:1227600/1‑92 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTa                        >  2:629201/1‑130 (MQ=255)
                                                                                                  tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTa                        <  1:629201/130‑1 (MQ=255)
                                                                                                     gcgcCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCt            <  2:47464/139‑1 (MQ=255)
                                                                                                     gcgcCGCCAGACCAGCCGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGTGCt            <  2:930715/139‑1 (MQ=255)
                                                                                                       gccgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa          >  2:245510/1‑139 (MQ=255)
                                                                                                         cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCAt                                                                                                   <  2:18608/48‑1 (MQ=255)
                                                                                                         cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCAt                                                                                                   >  1:18608/1‑48 (MQ=255)
                                                                                                               aCCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                              <  2:1102887/47‑1 (MQ=255)
                                                                                                               aCCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                              >  1:1102887/1‑47 (MQ=255)
                                                                                                               aCCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGc  >  1:623931/1‑139 (MQ=255)
                                                                                                                   gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcagc                                                                                             >  2:1116017/1‑44 (MQ=255)
                                                                                                                   gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcagc                                                                                             <  1:1116017/44‑1 (MQ=255)
                                                                                                                   gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                   >  2:9447/1‑54 (MQ=255)
                                                                                                                   gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                   <  1:9447/54‑1 (MQ=255)
                                                                                                                           |                                                                                                                              
ACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGC  >  NC_000913/1841774‑1842023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: