Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,909,123	C→T	P67S (CCG→TCG)	yobH →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,909,123	0	C	T	100.0%	199.0 / NA	62	P67S (CCG→TCG)	yobH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/33);  total (29/33)

GTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTA  >  NC_000913/1908997‑1909224                                                                                                                               |                                                                                                      gTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCa                                                                                           >  2:704336/1‑139 (MQ=255)  ttttGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCaa                                                                                          <  1:1347869/139‑1 (MQ=255)          ggAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAata                                                                                  >  1:306896/1‑139 (MQ=255)             aGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCg                                                                               <  1:1043820/139‑1 (MQ=255)               cAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTg                                                                             <  2:1287704/139‑1 (MQ=255)                 aaGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGt                                                                           <  2:1044362/139‑1 (MQ=255)                 aaGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGt                                                                           <  2:511398/139‑1 (MQ=255)                     gaATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGAt                                                                       <  2:631293/139‑1 (MQ=255)                       aTGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     >  2:503417/1‑139 (MQ=255)                        tGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAAt                                                                    >  2:759313/1‑139 (MQ=255)                            gCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGtt                                                                <  2:1018209/139‑1 (MQ=255)                                gATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGt                                                                                                 >  2:401603/1‑102 (MQ=255)                                gATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGt                                                                                                 <  1:401603/102‑1 (MQ=255)                                  ttAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGc                                                          <  1:503417/139‑1 (MQ=255)                                   tAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCa                                                         <  2:672474/139‑1 (MQ=255)                                          gCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATc                                                                                >  2:311519/1‑109 (MQ=255)                                          gCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATc                                                                                <  1:311519/109‑1 (MQ=255)                                                tACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGt                                                                           >  2:255712/1‑108 (MQ=255)                                                tACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGt                                                                           <  1:255712/108‑1 (MQ=255)                                                    tATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATtct                                        <  1:759313/139‑1 (MQ=255)                                                     aTCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATc                                                                                >  2:296313/1‑98 (MQ=255)                                                     aTCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATc                                                                                <  1:296313/98‑1 (MQ=255)                                                         gACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTc                                   <  1:1188262/139‑1 (MQ=255)                                                          aCTGCCAGCGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTcc                                  >  2:890097/1‑139 (MQ=255)                                                          aCTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     <  1:458415/104‑1 (MQ=255)                                                          aCTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     >  1:107994/1‑104 (MQ=255)                                                          aCTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     <  2:107994/104‑1 (MQ=255)                                                          aCTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     >  2:458415/1‑104 (MQ=255)                                                             gCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATa                                                                      <  2:1102507/100‑1 (MQ=255)                                                             gCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATa                                                                      >  1:1102507/1‑100 (MQ=255)                                                             gCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTATCACTCCTGa                               >  1:424184/1‑139 (MQ=255)                                                                agagGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGTGCAATAAATCACCAGATTCTCACTCCTGAgaa                            >  2:9002/1‑137 (MQ=255)                                                                    gCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATa                                                                      >  1:1181451/1‑93 (MQ=255)                                                                    gCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATa                                                                      <  2:1181451/93‑1 (MQ=255)                                                                        cAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCg                                                                               >  2:328192/1‑80 (MQ=255)                                                                        cAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCg                                                                               <  1:328192/80‑1 (MQ=255)                                                                        cAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGc                                                          >  2:1130839/1‑101 (MQ=255)                                                                        cAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGc                                                          <  1:1130839/101‑1 (MQ=255)                                                                              ggTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAg              <  1:704336/139‑1 (MQ=255)                                                                                 aCAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTT‑AGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAg          <  1:574578/139‑1 (MQ=255)                                                                                        ttGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGcgcgct    <  1:819852/139‑3 (MQ=255)                                                                                        ttGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGtt    >  2:819852/1‑139 (MQ=255)                                                                                         tGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCt                                 >  1:1062157/1‑109 (MQ=255)                                                                                         tGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCt                                 <  2:1062157/109‑1 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     <  2:1310992/72‑1 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATaa                                                                     >  1:1310992/1‑72 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATtct                                        <  1:448776/101‑1 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATtct                                        >  2:448776/1‑101 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAAt                        <  2:934515/117‑1 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAgc         >  2:1277875/1‑132 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAgc         <  1:1277875/132‑1 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTa  >  2:242581/1‑139 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCCTATAGCAAGCGCGTTTa  >  1:873006/1‑139 (MQ=255)                                                                                          gCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGAGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAAt                        >  1:934515/1‑117 (MQ=255)                                                                                                              tGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCt                                 >  1:723836/1‑88 (MQ=255)                                                                                                              tGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCt                                 <  2:723836/88‑1 (MQ=255)                                                                                                              tGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTaaaa                         <  2:673951/96‑1 (MQ=255)                                                                                                              tGGGGATAACAGATTGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTaaaa                         >  1:673951/1‑96 (MQ=255)                                                                                                                           ttGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCt                                 <  2:1204207/75‑1 (MQ=255)                                                                                                                           ttGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCt                                 >  1:1204207/1‑75 (MQ=255)                                                                                                                            tGCTCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATa                       >  2:73147/1‑84 (MQ=255)                                                                                                                            tGCTCGCTGTTACGCAAAAGCAAGATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATa                       <  1:73147/84‑1 (MQ=255)                                                                                                                               |                                                                                                      GTTTTGATTGGAAGCAAAGAGAATGCCGCAGGATTAGGTTTGCAATGTACCTATCTGACTGCCAGAGGCACCAGCACGGTACAATACTTGCACACTAAAAGTGGTTTTTTGGGGATAACAGATTGCCCGCTGTTACGCAAAAGCAATATCGTGGTCGATAATGGTTAAGGGCAATAAATAACCAGATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTA  >  NC_000913/1908997‑1909224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑